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Mix-n-Stain CF Dye Antibody Labeling Kits

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF™ dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

Mix-n-Stain CF Dye Antibody Labeling Kits
Catalog #

92333, 92285, 92284, 92283, 92282, 92281, 92280, 92279, 92278, 92277, 92276, 92275, 92274, 92273, 92272, 92271, 92270, 92287, 92288, 92289, 92332, 92331, 92324, 92323, 92322, 92321, 92320, 92319, 92318, 92317, 92316, 92305, 92304, 92303, 92291, 92290, 92270, 92268, 92267, 92246, 92245, 92243, 92242, 92241, 92240, 92239, 92238, 92237, 92236, 92235, 92234, 92233, 92232, 92231, 92230, 92247, 92248, 92250, 92265, 92264, 92263, 92262, 92261, 92260, 92259, 92258, 92257, 92256, 92255, 92254, 92253, 92252, 92251, 92250, - 92230View allHide

Size

5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labeling

Conjugation

CF350, CF405L, CF405M, CF405S, CF430, CF440, CF450, CF488A, CF514, CF532, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF660C, CF660R, CF680, CF680R, CF750, CF770, CF790

Conjugation
Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF™ dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label 5-20 ug, 20-50 ug, or 50-100 ug of your antibody. For more information and guidelines for selecting a kit size, please download the Mix-n-Stain™ flyer and the product information sheet for this product. Mix-n-Stain™ CF™ Dye and Biotin Antibody Labeling Kits now have an extended shelf life of at least 1 year from date of receipt when stored as recommended.

The kit is available with the following CF™ dyes: CF™350, CF™405S, CF™405M, CF™405L, CF™430, CF™440, CF™450, CF™488A, CF™514, CF™532, CF™543, CF™555, CF™568, CF™594, CF™633, CF™640R, CF™647, CF™660C, CF™660R, CF™680, CF™680R, CF™750, CF™770, and CF™790.

 

Mix-n-Stain CF Dye Antibody Labeling Kits: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm50-100 μg92230Purchase 92230
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm50-100 μg92230Purchase 92230
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm20-50 μg Labeling92250Purchase 92250
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm20-50 μg Labeling92250Purchase 92250
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm20-50 μg Labeling92270Purchase 92270
CF350 Mix-n-Stain CF Dye Antibody Labeling KitsCF350347/448 nm20-50 μg Labeling92270Purchase 92270
CF405L Mix-n-Stain CF Dye Antibody Labeling KitsCF405L395/545 nm20-50 μg Labeling92303Purchase 92303
CF405L Mix-n-Stain CF Dye Antibody Labeling KitsCF405L395/545 nm20-50 μg Labeling92304Purchase 92304
CF405L Mix-n-Stain CF Dye Antibody Labeling KitsCF405L395/545 nm50-100 μg92305Purchase 92305
CF405M Mix-n-Stain CF Dye Antibody Labeling KitsCF405M408/452 nm50-100 μg92232Purchase 92232
CF405M Mix-n-Stain CF Dye Antibody Labeling KitsCF405M408/452 nm20-50 μg Labeling92252Purchase 92252
CF405M Mix-n-Stain CF Dye Antibody Labeling KitsCF405M408/452 nm20-50 μg Labeling92272Purchase 92272
CF405S Mix-n-Stain CF Dye Antibody Labeling KitsCF405S404/431 nm50-100 μg92231Purchase 92231
CF405S Mix-n-Stain CF Dye Antibody Labeling KitsCF405S404/431 nm20-50 μg Labeling92251Purchase 92251
CF405S Mix-n-Stain CF Dye Antibody Labeling KitsCF405S404/431 nm20-50 μg Labeling92271Purchase 92271
CF430 Mix-n-Stain CF Dye Antibody Labeling KitsCF430426/498 nm20-50 μg Labeling92316Purchase 92316
CF430 Mix-n-Stain CF Dye Antibody Labeling KitsCF430426/498 nm20-50 μg Labeling92317Purchase 92317
CF430 Mix-n-Stain CF Dye Antibody Labeling KitsCF430426/498 nm50-100 μg92318Purchase 92318
CF440 Mix-n-Stain CF Dye Antibody Labeling KitsCF440440/515 nm20-50 μg Labeling92319Purchase 92319
CF440 Mix-n-Stain CF Dye Antibody Labeling KitsCF440440/515 nm20-50 μg Labeling92320Purchase 92320
CF440 Mix-n-Stain CF Dye Antibody Labeling KitsCF440440/515 nm50-100 μg92321Purchase 92321
CF450 Mix-n-Stain CF Dye Antibody Labeling KitsCF450405/460 nm20-50 μg Labeling92322Purchase 92322
CF450 Mix-n-Stain CF Dye Antibody Labeling KitsCF450405/460 nm20-50 μg Labeling92323Purchase 92323
CF450 Mix-n-Stain CF Dye Antibody Labeling KitsCF450405/460 nm50-100 μg92324Purchase 92324
CF488A Mix-n-Stain CF Dye Antibody Labeling KitsCF488A490/515 nm50-100 μg92233Purchase 92233
CF488A Mix-n-Stain CF Dye Antibody Labeling KitsCF488A490/515 nm20-50 μg Labeling92253Purchase 92253
CF488A Mix-n-Stain CF Dye Antibody Labeling KitsCF488A490/515 nm20-50 μg Labeling92273Purchase 92273
CF514 Mix-n-Stain CF Dye Antibody Labeling KitsCF514516/548 nm20-50 μg Labeling92331Purchase 92331
CF514 Mix-n-Stain CF Dye Antibody Labeling KitsCF514516/548 nm20-50 μg Labeling92332Purchase 92332
CF514 Mix-n-Stain CF Dye Antibody Labeling KitsCF514516/548 nm50-100 μg92333Purchase 92333
CF532 Mix-n-Stain CF Dye Antibody Labeling KitsCF532527/558 nm20-50 μg Labeling92289Purchase 92289
CF532 Mix-n-Stain CF Dye Antibody Labeling KitsCF532527/558 nm20-50 μg Labeling92290Purchase 92290
CF532 Mix-n-Stain CF Dye Antibody Labeling KitsCF532527/558 nm50-100 μg92291Purchase 92291
CF543 Mix-n-Stain CF Dye Antibody Labeling KitsCF543541/560 nm50-100 μg92247Purchase 92247
CF543 Mix-n-Stain CF Dye Antibody Labeling KitsCF543541/560 nm20-50 μg Labeling92267Purchase 92267
CF543 Mix-n-Stain CF Dye Antibody Labeling KitsCF543541/560 nm20-50 μg Labeling92287Purchase 92287
CF555 Mix-n-Stain CF Dye Antibody Labeling KitsCF555555/565 nm50-100 μg92234Purchase 92234
CF555 Mix-n-Stain CF Dye Antibody Labeling KitsCF555555/565 nm20-50 μg Labeling92254Purchase 92254
CF555 Mix-n-Stain CF Dye Antibody Labeling KitsCF555555/565 nm20-50 μg Labeling92274Purchase 92274
CF568 Mix-n-Stain CF Dye Antibody Labeling KitsCF568562/583 nm50-100 μg92235Purchase 92235
CF568 Mix-n-Stain CF Dye Antibody Labeling KitsCF568562/583 nm20-50 μg Labeling92255Purchase 92255
CF568 Mix-n-Stain CF Dye Antibody Labeling KitsCF568562/583 nm20-50 μg Labeling92275Purchase 92275
CF594 Mix-n-Stain CF Dye Antibody Labeling KitsCF594593/614 nm50-100 μg92236Purchase 92236
CF594 Mix-n-Stain CF Dye Antibody Labeling KitsCF594593/614 nm20-50 μg Labeling92256Purchase 92256
CF594 Mix-n-Stain CF Dye Antibody Labeling KitsCF594593/614 nm20-50 μg Labeling92276Purchase 92276
CF633 Mix-n-Stain CF Dye Antibody Labeling KitsCF633630/650 nm50-100 μg92237Purchase 92237
CF633 Mix-n-Stain CF Dye Antibody Labeling KitsCF633630/650 nm20-50 μg Labeling92257Purchase 92257
CF633 Mix-n-Stain CF Dye Antibody Labeling KitsCF633630/650 nm20-50 μg Labeling92277Purchase 92277
CF640R Mix-n-Stain CF Dye Antibody Labeling KitsCF640R642/662 nm50-100 μg92245Purchase 92245
CF640R Mix-n-Stain CF Dye Antibody Labeling KitsCF640R642/662 nm20-50 μg Labeling92258Purchase 92258
CF640R Mix-n-Stain CF Dye Antibody Labeling KitsCF640R642/662 nm20-50 μg Labeling92278Purchase 92278
CF647 Mix-n-Stain CF Dye Antibody Labeling KitsCF647650/665 nm50-100 μg92238Purchase 92238
CF647 Mix-n-Stain CF Dye Antibody Labeling KitsCF647650/665 nm20-50 μg Labeling92259Purchase 92259
CF647 Mix-n-Stain CF Dye Antibody Labeling KitsCF647650/665 nm20-50 μg Labeling92279Purchase 92279
CF660C Mix-n-Stain CF Dye Antibody Labeling KitsCF660C667/685 nm50-100 μg92239Purchase 92239
CF660C Mix-n-Stain CF Dye Antibody Labeling KitsCF660C667/685 nm20-50 μg Labeling92260Purchase 92260
CF660C Mix-n-Stain CF Dye Antibody Labeling KitsCF660C667/685 nm20-50 μg Labeling92280Purchase 92280
CF660R Mix-n-Stain CF Dye Antibody Labeling KitsCF660R663/682 nm50-100 μg92243Purchase 92243
CF660R Mix-n-Stain CF Dye Antibody Labeling KitsCF660R663/682 nm20-50 μg Labeling92261Purchase 92261
CF660R Mix-n-Stain CF Dye Antibody Labeling KitsCF660R663/682 nm20-50 μg Labeling92281Purchase 92281
CF680 Mix-n-Stain CF Dye Antibody Labeling KitsCF680681/698 nm50-100 μg92240Purchase 92240
CF680 Mix-n-Stain CF Dye Antibody Labeling KitsCF680681/698 nm20-50 μg Labeling92262Purchase 92262
CF680 Mix-n-Stain CF Dye Antibody Labeling KitsCF680681/698 nm20-50 μg Labeling92282Purchase 92282
CF680R Mix-n-Stain CF Dye Antibody Labeling KitsCF680R680/701 nm50-100 μg92246Purchase 92246
CF680R Mix-n-Stain CF Dye Antibody Labeling KitsCF680R680/701 nm20-50 μg Labeling92263Purchase 92263
CF680R Mix-n-Stain CF Dye Antibody Labeling KitsCF680R680/701 nm20-50 μg Labeling92283Purchase 92283
CF750 Mix-n-Stain CF Dye Antibody Labeling KitsCF750755/777 nm50-100 μg92241Purchase 92241
CF750 Mix-n-Stain CF Dye Antibody Labeling KitsCF750755/777 nm20-50 μg Labeling92264Purchase 92264
CF750 Mix-n-Stain CF Dye Antibody Labeling KitsCF750755/777 nm20-50 μg Labeling92284Purchase 92284
CF770 Mix-n-Stain CF Dye Antibody Labeling KitsCF770770/797 nm50-100 μg92242Purchase 92242
CF770 Mix-n-Stain CF Dye Antibody Labeling KitsCF770770/797 nm20-50 μg Labeling92265Purchase 92265
CF770 Mix-n-Stain CF Dye Antibody Labeling KitsCF770770/797 nm20-50 μg Labeling92285Purchase 92285
CF790 Mix-n-Stain CF Dye Antibody Labeling KitsCF790784/806 nm50-100 μg92248Purchase 92248
CF790 Mix-n-Stain CF Dye Antibody Labeling KitsCF790784/806 nm20-50 μg Labeling92268Purchase 92268
CF790 Mix-n-Stain CF Dye Antibody Labeling KitsCF790784/806 nm20-50 μg Labeling92288Purchase 92288

References

Download a list of CF dye references.

 

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    EverBrite Hardset Mounting Medium

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    Mix-n-Stain Fluorescent Protein & Tandem Dye Antibody Labeling Kits

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Support & Faq

Mix-n-Stain™ Antibody Labeling Kits

CF dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF dyes, the CF Dye Selection Guide, and our CF Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

← FAQs

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines. You can download CF™ dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

See the Mix-n-Stain Kit Selection Guide, below:

Antibody formulationKit and protocol selectionNotes/Examples
Purified IgG containing:

• Sodium azide

• Less than 20 mM Tris

• Less than 10% glycerol
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• More than 20 mM Tris

• More than 10% glycerol

• Glycine

• Less than 0.5 ug/uL IgG
1. Choose the kit size based on the amount of IgG you wish to label

2. Perform ultrafiltration using the spin vial provided in the kit

3. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• Less than 4:1 BSA:IgG by weight

• Less than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS contain­ing 0.1% BSA. The BSA:IgG ratio by weight is 5 ug BSA:5 ug IgG or 1:1. Select a 5-20 ug-sized kit and follow the standard protocol.
Purified IgG containing:

• More than 4:1 BSA:IgG by weight

• More than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the total amount of protein (IgG + BSA/gelatin) in the volume of antibody solution you wish to label

2. Use the modified labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS containing 1% BSA. The BSA:IgG ratio by weight is 50 ug BSA: 5 ug IgG or 10/1. Select a 50-100 ug-sized kit based on 55 ug of total protein in the labeling reaction and follow the modified labeling protocol. If the total protein amount falls between two kit sizes, you may get better results with the larger kit size.
IgG in ascites fluid1. Determine the concentration of protein in the ascites fluid

2. Choose the kit size based on the total amount of protein in the volume of ascites fluid you wish to label

3. Use the modified labeling protocol
For example, if you wish to label 10 uL ascites fluid containing 70 ug total protein. Select a 50-100 ug-sized kit based on 70 ug of total protein, and follow the modified labeling protocol.
Less than 5 ug purified IgG1. Add stabilizer protein such as BSA to the antibody to bring the total amount of protein 5 ug

2. Select a 5-20 ug kit and use the standard labeling protocol
For example, if you wish to label 1 ug IgG sample. Select a 5-20 ug-sized kit. Before labeling, add 4 ug BSA to 1 ug IgG and follow the standard labeling protocol.
IgG in:

• Serum

• Hybridoma cell culture supernatant
Not compatible due to the low concentration of IgG compared to other proteins in these formats; purify IgG before labelingUse protein A/G or a commercially available IgG clean-up kit to purify IgG. Determine the concentration of IgG, then select the appropriate kit/protocol based on the amount of IgG you wish to label and the buffer formulation after purification.

Category: Mix-n-Stain™ Antibody Labeling Kits

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← FAQs

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CF™ Dyes

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines. You can download CF™ dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

CF initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF dyes based on cyanine dye structures. Since then, our CF dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF™ Dyes

← FAQs

The exact chemical structures of CF dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF dye invention that makes CF dyes superior to other commercial dyes.

Category: CF™ Dyes

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.