Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to covalently label as little as ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification. The kits tolerate common antibody buffer formulations. With a slight modification in protocol, antibodies even can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience.
These kits feature a choice of 26 of Biotium’s next-generation CF® dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Because CF® dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because non-antibody proteins readily wash away during IF staining.
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Choice of 26 CF® Dye colors
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification of the product is required, for 100% recovery
- Compatible with common antibody stabilizers
See table below for a quick reference of the maximum excitation and emission of each CF® dye, or see our CF® Dye Brochure for more detailed dye information. For more information and guidelines for selecting a kit size, please download the Mix-n-Stain™ flyer and the product information sheet for this product. We also offer Mix-n-Stain™ kits for labeling antibodies with enzymes, fluorescent proteins, biotin, and DIG. See the product table below.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Mix-n-Stain™ Antibody Labeling Kits
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.