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Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Label your IgG antibody with one of our superior CF® Dyes in as little as 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

Conjugation
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Size
Catalog #
price
Qty
3x(5-20 ug) labelings
5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your IgG antibody in as little as 15 minutes, with minimal hands-on time and no purification.

Note: This kit is for labeling IgG antibodies only. For labeling IgM antibodies, please see our Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Features

  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of over 30 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 15 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol
Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Superior CF® Dyes

These kits feature a choice of 31 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of IgG antibody with CF® Dyes, as well as Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm5-20 ug92270CF®350 Features
20-50 ug92250
50-100 ug92230
CF®405S404/431 nm5-20 ug92271CF®405S Features
20-50 ug92251
50-100 ug92231
CF®405M408/452 nm5-20 ug92272CF®405M Features
20-50 ug92252
50-100 ug92232
CF®405L395/545 nm5-20 ug92303CF®405L Features
20-50 ug92304
50-100 ug92305
3x(5-20 ug)92445
CF®430426/498 nm5-20 ug92316CF®430 Features
20-50 ug92317
50-100 ug92318
CF®440440/515 nm5-20 ug92319CF®440 Features
20-50 ug92320
50-100 ug92321
CF®450450/538 nm5-20 ug92322CF®450 Features
20-50 ug92323
50-100 ug92324
CF®488A490/515 nm5-20 ug92273CF®488A Features
20-50 ug92253
50-100 ug92233
3x(5-20 ug)92446
CF®514516/548 nm5-20 ug92331CF®514 Features
20-50 ug92332
50-100 ug92333
CF®532527/558 nm5-20 ug92289CF®532 Features
20-50 ug92290
50-100 ug92291
CF®543541/560 nm5-20 ug92287CF®543 Features
20-50 ug92267
50-100 ug92247
CF®555555/565 nm5-20 ug92274CF®555 Features
20-50 ug92254
50-100 ug92234
CF®568562/583 nm5-20 ug92275CF®568 Features
20-50 ug92255
50-100 ug92235
3x(5-20 ug)92447
CF®570568/591 nm5-20 ug92334CF®570 Features
20-50 ug92335
50-100 ug92336
CF®583583/606 nm5-20 ug92337CF®583 Features
20-50 ug92338
50-100 ug92339
CF®583R586/609 nm5-20 ug92455CF®583R Features
20-50 ug92456
50-100 ug92457
CF®594593/614 nm5-20 ug92276CF®594 Features
20-50 ug92256
50-100 ug92236
3x(5-20 ug)92448
CF®597R597/619 nm5-20 ug92461CF®597R Features
20-50 ug92462
50-100 ug92463
CF®633630/650 nm5-20 ug92277CF®633 Features
20-50 ug92257
50-100 ug92237
CF®640R642/662 nm5-20 ug92278CF®640R Features
20-50 ug92258
50-100 ug92245
CF®647650/665 nm5-20 ug92279CF®647 Features
20-50 ug92259
50-100 ug92238
3x(5-20 ug)92449
CF®660R663/682 nm5-20 ug92281CF®660R Features
20-50 ug92261
50-100 ug92243
CF®660C667/685 nm5-20 ug92280CF®660C Features
20-50 ug92260
50-100 ug92239
CF®680681/698 nm5-20 ug92282CF®680 Features
20-50 ug92262
50-100 ug92240
CF®680R680/701 nm5-20 ug92283CF®680R Features
20-50 ug92263
50-100 ug92246
CF®700695/720 nm5-20 ug92425CF®700 Features
20-50 ug92426
50-100 ug92427
CF®750755/777 nm5-20 ug92284CF®750 Features
20-50 ug92264
50-100 ug92241
CF®770770/797 nm5-20 ug92285CF®770 Features
20-50 ug92265
50-100 ug92242
CF®790784/806 nm5-20 ug92288CF®790 Features
20-50 ug92268
50-100 ug92248
CF®800797/816 nm5-20 ug92428CF®800 Features
20-50 ug92429
50-100 ug92430
CF®820822/835 nm5-20 ug92431CF®820 Features
20-50 ug92432
50-100 ug92433
* Download the Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of Mix-n-Stain™ references.

 

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