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Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Label your antibody with one of our superior CF® dyes in 30 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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3x(5-20 ug) labelings
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20-50 ug labeling
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Product Description

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.


  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 29 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol
Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Superior CF® Dyes

These kits feature a choice of 29 of Biotium’s next-generation CF® dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

See the product table below for a quick reference of the maximum excitation and emission of each CF® dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

ProductDye Ex/EmLabeling size
(ug antibody)*
Catalog number
CF®350 Mix-n-Stain™ Antibody Labeling Kits347/448 nm5-20 ugPurchase 92270
20-50 ugPurchase 92250
50-100 ugPurchase 92230
CF®405S Mix-n-Stain™ Antibody Labeling Kits404/431 nm5-20 ugPurchase 92271
20-50 ugPurchase 92251
50-100 ugPurchase 92231
CF®405M Mix-n-Stain™ Antibody Labeling Kits408/452 nm5-20 ugPurchase 92272
20-50 ugPurchase 92252
50-100 ugPurchase 92232
CF®405L Mix-n-Stain™ Antibody Labeling Kits395/545 nm5-20 ugPurchase 92303
20-50 ugPurchase 92304
50-100 ugPurchase 92305
3x(5-20 ug)Purchase 92445
CF®430 Mix-n-Stain™ Antibody Labeling Kits426/498 nm5-20 ugPurchase 92316
20-50 ugPurchase 92317
50-100 ugPurchase 92318
CF®440 Mix-n-Stain™ Antibody Labeling Kits440/515 nm5-20 ugPurchase 92319
20-50 ugPurchase 92320
50-100 ugPurchase 92321
CF®450 Mix-n-Stain™ Antibody Labeling Kits450/538 nm5-20 ugPurchase 92322
20-50 ugPurchase 92323
50-100 ugPurchase 92324
CF®488A Mix-n-Stain™ Antibody Labeling Kits490/515 nm5-20 ugPurchase 92273
20-50 ugPurchase 92253
50-100 ugPurchase 92233
3x(5-20 ug)Purchase 92446
CF®514 Mix-n-Stain™ Antibody Labeling Kits516/548 nm5-20 ugPurchase 92331
20-50 ugPurchase 92332
50-100 ugPurchase 92333
CF®532 Mix-n-Stain™ Antibody Labeling Kits527/558 nm5-20 ugPurchase 92289
20-50 ugPurchase 92290
50-100 ugPurchase 92291
CF®543 Mix-n-Stain™ Antibody Labeling Kits541/560 nm5-20 ugPurchase 92287
20-50 ugPurchase 92267
50-100 ugPurchase 92247
CF®555 Mix-n-Stain™ Antibody Labeling Kits555/565 nm5-20 ugPurchase 92274
20-50 ugPurchase 92254
50-100 ugPurchase 92234
CF®568 Mix-n-Stain™ Antibody Labeling Kits562/583 nm5-20 ugPurchase 92275
20-50 ugPurchase 92255
50-100 ugPurchase 92235
3x(5-20 ug)Purchase 92447
CF®570 Mix-n-Stain™ Antibody Labeling Kits568/591 nm5-20 ugPurchase 92334
20-50 ugPurchase 92335
50-100 ugPurchase 92336
CF®583 Mix-n-Stain™ Antibody Labeling Kits583/606 nm5-20 ugPurchase 92337
20-50 ugPurchase 92338
50-100 ugPurchase 92339
CF®594 Mix-n-Stain™ Antibody Labeling Kits593/614 nm5-20 ugPurchase 92276
20-50 ugPurchase 92256
50-100 ugPurchase 92236
3x(5-20 ug)Purchase 92448
CF®633 Mix-n-Stain™ Antibody Labeling Kits630/650 nm5-20 ugPurchase 92277
20-50 ugPurchase 92257
50-100 ugPurchase 92237
CF®640R Mix-n-Stain™ Antibody Labeling Kits642/662 nm5-20 ugPurchase 92278
20-50 ugPurchase 92258
50-100 ugPurchase 92245
CF®647 Mix-n-Stain™ Antibody Labeling Kits650/665 nm5-20 ugPurchase 92279
20-50 ugPurchase 92259
50-100 ugPurchase 92238
3x(5-20 ug)Purchase 92449
CF®660C Mix-n-Stain™ Antibody Labeling Kits667/685 nm5-20 ugPurchase 92280
20-50 ugPurchase 92260
50-100 ugPurchase 92239
CF®660R Mix-n-Stain™ Antibody Labeling Kits663/682 nm5-20 ugPurchase 92281
20-50 ugPurchase 92261
50-100 ugPurchase 92243
CF®680 Mix-n-Stain™ Antibody Labeling Kits681/698 nm5-20 ugPurchase 92282
20-50 ugPurchase 92262
50-100 ugPurchase 92240
CF®680R Mix-n-Stain™ Antibody Labeling Kits680/701 nm5-20 ugPurchase 92283
20-50 ugPurchase 92263
50-100 ugPurchase 92246
CF®700 Mix-n-Stain™ Antibody Labeling Kits695/720 nm5-20 ugPurchase 92425
20-50 ugPurchase 92426
50-100 ugPurchase 92427
CF®750 Mix-n-Stain™ Antibody Labeling Kits755/777 nm5-20 ugPurchase 92284
20-50 ugPurchase 92264
50-100 ugPurchase 92241
CF®770 Mix-n-Stain™ Antibody Labeling Kits770/797 nm5-20 ugPurchase 92285
20-50 ugPurchase 92265
50-100 ugPurchase 92242
CF®790 Mix-n-Stain™ Antibody Labeling Kits784/806 nm5-20 ugPurchase 92288
20-50 ugPurchase 92268
50-100 ugPurchase 92248
CF®800 Mix-n-Stain™ Antibody Labeling Kits797/816 nm5-20 ugPurchase 92428
20-50 ugPurchase 92429
50-100 ugPurchase 92430
CF®820 Mix-n-Stain™ Antibody Labeling Kits822/835 nm5-20 ugPurchase 92431
20-50 ugPurchase 92432
50-100 ugPurchase 92433
* Download the Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.


Download a list of Mix-n-Stain™ references.


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