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Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

Features:

  • 3 kit sizes: 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 24 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification of the product is required
  • Compatible with many common buffer components and stabilizers
Conjugation
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5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to covalently label as little as ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification. The kits tolerate common antibody buffer formulations. With a slight modification in protocol, antibodies even can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience.

These kits feature a choice of 26 of Biotium’s next-generation CF® dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Because CF® dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because non-antibody proteins readily wash away during IF staining.

Features

  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 26 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification of the product is required, for 100% recovery
  • Compatible with common antibody stabilizers

See table below for a quick reference of the maximum excitation and emission of each CF® dye, or see our CF® Dye Brochure for more detailed dye information. For more information and guidelines for selecting a kit size, please download the Mix-n-Stain™ flyer and the product information sheet for this product. We also offer Mix-n-Stain™ kits for labeling antibodies with enzymes, fluorescent proteins, biotin, and DIG. See the product table  below.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

ProductDyeEx/EmLabeling size
(ug antibody)
Catalog number
CF®350 Mix-n-Stain™ Antibody Labeling KitsCF®350347/448 nm5-20 ugPurchase 92270
20-50 ugPurchase 92250
50-100 ugPurchase 92230
CF®405S Mix-n-Stain™ Antibody Labeling KitsCF®405S404/431 nm5-20 ugPurchase 92271
20-50 ugPurchase 92251
50-100 ugPurchase 92231
CF®405M Mix-n-Stain™ Antibody Labeling KitsCF®405M408/452 nm5-20 ugPurchase 92272
20-50 ugPurchase 92252
50-100 ugPurchase 92232
CF®405L Mix-n-Stain™ Antibody Labeling KitsCF®405L395/545 nm5-20 ugPurchase 92303
20-50 ugPurchase 92304
50-100 ugPurchase 92305
CF®430 Mix-n-Stain™ Antibody Labeling KitsCF®430426/498 nm5-20 ugPurchase 92316
20-50 ugPurchase 92317
50-100 ugPurchase 92318
CF®440 Mix-n-Stain™ Antibody Labeling KitsCF®440440/515 nm5-20 ugPurchase 92319
20-50 ugPurchase 92320
50-100 ugPurchase 92321
CF®450 Mix-n-Stain™ Antibody Labeling KitsCF®450405/460 nm5-20 ugPurchase 92322
20-50 ugPurchase 92323
50-100 ugPurchase 92324
CF®488A Mix-n-Stain™ Antibody Labeling KitsCF®488A490/515 nm5-20 ugPurchase 92273
20-50 ugPurchase 92253
50-100 ugPurchase 92233
CF®514 Mix-n-Stain™ Antibody Labeling KitsCF®514516/548 nm5-20 ugPurchase 92331
20-50 ugPurchase 92332
50-100 ugPurchase 92333
CF®532 Mix-n-Stain™ Antibody Labeling KitsCF®532527/558 nm5-20 ugPurchase 92289
20-50 ugPurchase 92290
50-100 ugPurchase 92291
CF®543 Mix-n-Stain™ Antibody Labeling KitsCF®543541/560 nm5-20 ugPurchase 92287
20-50 ugPurchase 92267
50-100 ugPurchase 92247
CF®555 Mix-n-Stain™ Antibody Labeling KitsCF®555555/565 nm5-20 ugPurchase 92274
20-50 ugPurchase 92254
50-100 ugPurchase 92234
CF®568 Mix-n-Stain™ Antibody Labeling KitsCF®568562/583 nm5-20 ugPurchase 92275
20-50 ugPurchase 92255
50-100 ugPurchase 92235
CF®570 Mix-n-Stain™ Antibody Labeling KitsCF®570568/591 nm5-20 ugPurchase 92334
20-50 ugPurchase 92335
50-100 ugPurchase 92336
CF®583 Mix-n-Stain™ Antibody Labeling KitsCF®583583/606 nm5-20 ugPurchase 92237
20-50 ugPurchase 92238
50-100 ugPurchase 92239
CF®594 Mix-n-Stain™ Antibody Labeling KitsCF®594593/614 nm5-20 ugPurchase 92276
20-50 ugPurchase 92256
50-100 ugPurchase 92236
CF®633 Mix-n-Stain™ Antibody Labeling KitsCF®633630/650 nm5-20 ugPurchase 92277
20-50 ugPurchase 92257
50-100 ugPurchase 92237
CF®640R Mix-n-Stain™ Antibody Labeling KitsCF®640R642/662 nm5-20 ugPurchase 92278
20-50 ugPurchase 92258
50-100 ugPurchase 92245
CF®647 Mix-n-Stain™ Antibody Labeling KitsCF®647650/665 nm5-20 ugPurchase 92279
20-50 ugPurchase 92259
50-100 ugPurchase 92238
CF®660C Mix-n-Stain™ Antibody Labeling KitsCF®660C667/685 nm5-20 ugPurchase 92280
20-50 ugPurchase 92260
50-100 ugPurchase 92239
CF®660R Mix-n-Stain™ Antibody Labeling KitsCF®660R663/682 nm5-20 ugPurchase 92281
20-50 ugPurchase 92261
50-100 ugPurchase 92243
CF®680 Mix-n-Stain™ Antibody Labeling KitsCF®680681/698 nm5-20 ugPurchase 92282
20-50 ugPurchase 92262
50-100 ugPurchase 92240
CF®680R Mix-n-Stain™ Antibody Labeling KitsCF®680R680/701 nm5-20 ugPurchase 92283
20-50 ugPurchase 92263
50-100 ugPurchase 92246
CF®750 Mix-n-Stain™ Antibody Labeling KitsCF®750755/777 nm5-20 ugPurchase 92284
20-50 ugPurchase 92264
50-100 ugPurchase 92241
CF®770 Mix-n-Stain™ Antibody Labeling KitsCF®770770/797 nm5-20 ugPurchase 92285
20-50 ugPurchase 92265
50-100 ugPurchase 92242
CF®790 Mix-n-Stain™ Antibody Labeling KitsCF®790784/806 nm5-20 ugPurchase 92288
20-50 ugPurchase 92268
50-100 ugPurchase 92248

 

Mix-n-Stain™ Antibody Labeling Kits

Product NameLabel Options
Mix-n-Stain™ CF® Dye Antibody Labeling KitsChoice of 24 bright and stable CF® Dyes
Mix-n-Stain™ FITC Antibody Labeling KitsFITC
Mix-n-Stain™ Biotin Antibody Labeling KitsBiotin
Mix-n-Stain™ Digoxigenin Antibody Labeling KitsDigoxigenin (DIG)
Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling KitsAPC, R-PE, PerCP, APC-CF®750T, RPE-CF®647T
Mix-n-Stain™ Enzyme Dye Antibody Labeling KitsHRP, AP, GOx
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of Mix-n-Stain™ references.

 

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