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Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

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Features:

  • 3 kit sizes: 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 24 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification of the product is required
  • Compatible with many common buffer components and stabilizers
Conjugation

CF®350, CF®405L, CF®405M, CF®405S, CF®430, CF®440, CF®450, CF®488A, CF®514, CF®532, CF®543, CF®555, CF®568, CF®570, CF®583, CF®594, CF®633, CF®640R, CF®647, CF®660C, CF®660R, CF®680, CF®680R, CF®750, CF®770, CF®790

Conjugation
Size
Catalog #
price
Qty
5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired.

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Product Description

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label 5-20 ug, 20-50 ug, or 50-100 ug of your antibody, and from 24 next-generation fluorescent CF® dyes.

See table below for a quick reference of the maximum excitation and emission of each CF® dye, or see our CF® Dye Brochure for more detailed dye information. For more information and guidelines for selecting a kit size, please download the Mix-n-Stain™ flyer and the product information sheet for this product. We also offer Mix-n-Stain™ kits for labeling antibodies with enzymes, fluorescent proteins, biotin, and DIG. See list below.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

ProductDyeEx/Em Labeling size
(ug antibody)
Catalog number
CF®350 Mix-n-Stain™ Antibody Labeling KitsCF®350347/448 nm5-20 ugPurchase 92270
20-50 ugPurchase 92250
50-100 ugPurchase 92230
CF®405M Mix-n-Stain™ Antibody Labeling KitsCF®405M408/452 nm5-20 ugPurchase 92272
20-50 ugPurchase 92252
50-100 ugPurchase 92232
CF®405S Mix-n-Stain™ Antibody Labeling KitsCF®405S404/431 nm5-20 ugPurchase 92271
20-50 ugPurchase 92251
50-100 ugPurchase 92231
CF®405L Mix-n-Stain™ Antibody Labeling KitsCF®405L395/545 nm5-20 ugPurchase 92303
20-50 ugPurchase 92304
50-100 ugPurchase 92305
CF®430 Mix-n-Stain™ Antibody Labeling KitsCF®430426/498 nm5-20 ugPurchase 92316
20-50 ugPurchase 92317
50-100 ugPurchase 92318
CF®440 Mix-n-Stain™ Antibody Labeling KitsCF®440440/515 nm5-20 ugPurchase 92319
20-50 ugPurchase 92320
50-100 ugPurchase 92321
CF®450 Mix-n-Stain™ Antibody Labeling KitsCF®450405/460 nm5-20 ugPurchase 92322
20-50 ugPurchase 92323
50-100 ugPurchase 92324
CF®488A Mix-n-Stain™ Antibody Labeling KitsCF®488A490/515 nm5-20 ugPurchase 92273
20-50 ugPurchase 92253
50-100 ugPurchase 92233
CF®514 Mix-n-Stain™ Antibody Labeling KitsCF®514516/548 nm5-20 ugPurchase 92331
20-50 ugPurchase 92332
50-100 ugPurchase 92333
CF®532 Mix-n-Stain™ Antibody Labeling KitsCF®532527/558 nm5-20 ugPurchase 92289
20-50 ugPurchase 92290
50-100 ugPurchase 92291
CF®543 Mix-n-Stain™ Antibody Labeling KitsCF®543541/560 nm5-20 ugPurchase 92287
20-50 ugPurchase 92267
50-100 ugPurchase 92247
CF®555 Mix-n-Stain™ Antibody Labeling KitsCF®555555/565 nm5-20 ugPurchase 92274
20-50 ugPurchase 92254
50-100 ugPurchase 92234
CF®568 Mix-n-Stain™ Antibody Labeling KitsCF®568562/583 nm5-20 ugPurchase 92275
20-50 ugPurchase 92255
50-100 ugPurchase 92235
CF®594 Mix-n-Stain™ Antibody Labeling KitsCF®594593/614 nm5-20 ugPurchase 92276
20-50 ugPurchase 92256
50-100 ugPurchase 92236
CF®633 Mix-n-Stain™ Antibody Labeling KitsCF®633630/650 nm5-20 ugPurchase 92277
20-50 ugPurchase 92257
50-100 ugPurchase 92237
CF®640R Mix-n-Stain™ Antibody Labeling KitsCF®640R642/662 nm5-20 ugPurchase 92278
20-50 ugPurchase 92258
50-100 ugPurchase 92245
CF®647 Mix-n-Stain™ Antibody Labeling KitsCF®647650/665 nm5-20 ugPurchase 92279
20-50 ugPurchase 92259
50-100 ugPurchase 92238
CF®660C Mix-n-Stain™ Antibody Labeling KitsCF®660C667/685 nm5-20 ugPurchase 92280
20-50 ugPurchase 92260
50-100 ugPurchase 92239
CF®660R Mix-n-Stain™ Antibody Labeling KitsCF®660R663/682 nm5-20 ugPurchase 92281
20-50 ugPurchase 92261
50-100 ugPurchase 92243
CF®680 Mix-n-Stain™ Antibody Labeling KitsCF®680681/698 nm5-20 ugPurchase 92282
20-50 ugPurchase 92262
50-100 ugPurchase 92240
CF®680R Mix-n-Stain™ Antibody Labeling KitsCF®680R680/701 nm5-20 ugPurchase 92283
20-50 ugPurchase 92263
50-100 ugPurchase 92246
CF®750 Mix-n-Stain™ Antibody Labeling KitsCF®750755/777 nm5-20 ugPurchase 92284
20-50 ugPurchase 92264
50-100 ugPurchase 92241
CF®770 Mix-n-Stain™ Antibody Labeling KitsCF®770770/797 nm5-20 ugPurchase 92285
20-50 ugPurchase 92265
50-100 ugPurchase 92242
CF®790 Mix-n-Stain™ Antibody Labeling KitsCF®790784/806 nm5-20 ugPurchase 92288
20-50 ugPurchase 92268
50-100 ugPurchase 92248

Mix-n-Stain Antibody Labeling Kits

Product NameLabel Options
Mix-n-Stain™ CF® Dye Antibody Labeling KitsChoice of 24 bright and stable CF® Dyes
Mix-n-Stain™ FITC Antibody Labeling KitsFITC
Mix-n-Stain™ Biotin Antibody Labeling KitsBiotin
Mix-n-Stain™ Digoxigenin Antibody Labeling KitsDigoxigenin (DIG)
Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling KitsAPC, R-PE, PerCP, APC-CF®750T, RPE-CF®647T
Mix-n-Stain™ Enzyme Dye Antibody Labeling KitsHRP, AP, GOx

References

Download a list of Mix-n-Stain™ references.

 

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Support & Faq

Mix-n-Stain™ Antibody Labeling Kits

CF® dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF® dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF® dyes, the CF® Dye Selection Guide, and our CF® Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

← FAQs

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs

See the Mix-n-Stain™ Kit Selection Guide, below:

Antibody formulationKit and protocol selectionNotes/Examples
Purified IgG containing:

• Sodium azide

• Less than 20 mM Tris

• Less than 10% glycerol
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• More than 20 mM Tris

• More than 10% glycerol

• Glycine

• Less than 0.5 ug/uL IgG
1. Choose the kit size based on the amount of IgG you wish to label

2. Perform ultrafiltration using the spin vial provided in the kit

3. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• Less than 4:1 BSA:IgG by weight

• Less than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS contain­ing 0.1% BSA. The BSA:IgG ratio by weight is 5 ug BSA:5 ug IgG or 1:1. Select a 5-20 ug-sized kit and follow the standard protocol.
Purified IgG containing:

• More than 4:1 BSA:IgG by weight

• More than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the total amount of protein (IgG + BSA/gelatin) in the volume of antibody solution you wish to label

2. Use the modified labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS containing 1% BSA. The BSA:IgG ratio by weight is 50 ug BSA: 5 ug IgG or 10/1. Select a 50-100 ug-sized kit based on 55 ug of total protein in the labeling reaction and follow the modified labeling protocol. If the total protein amount falls between two kit sizes, you may get better results with the larger kit size.
IgG in ascites fluid1. Determine the concentration of protein in the ascites fluid

2. Choose the kit size based on the total amount of protein in the volume of ascites fluid you wish to label

3. Use the modified labeling protocol
For example, if you wish to label 10 uL ascites fluid containing 70 ug total protein. Select a 50-100 ug-sized kit based on 70 ug of total protein, and follow the modified labeling protocol.
Less than 5 ug purified IgG1. Add stabilizer protein such as BSA to the antibody to bring the total amount of protein 5 ug

2. Select a 5-20 ug kit and use the standard labeling protocol
For example, if you wish to label 1 ug IgG sample. Select a 5-20 ug-sized kit. Before labeling, add 4 ug BSA to 1 ug IgG and follow the standard labeling protocol.
IgG in:

• Serum

• Hybridoma cell culture supernatant
Not compatible due to the low concentration of IgG compared to other proteins in these formats; purify IgG before labelingUse protein A/G or a commercially available IgG clean-up kit to purify IgG. Determine the concentration of IgG, then select the appropriate kit/protocol based on the amount of IgG you wish to label and the buffer formulation after purification.

Category: Mix-n-Stain™ Antibody Labeling Kits

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CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs

CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.