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Thiol-Reactive Dyes

Maleimide reacts with thiol groups to form thioether-coupled products. The reaction can take place at pH 7 in the presence of amines. At neutral pH, the maleimide group does not react with histidine or arginine. Maleimide labeling can be a useful alternative to succinimidyl ester labeling in cases where modification of lysines alters antibody binding affinity or other biological activity of the target protein. We offer more than 20 bright and photostable CF® Dye Maleimides.

Methanethiosulfonate (MTS) is a fast-reacting and highly selective thiol-reactive functional group. We offer CF® Dye MTS with a selection of popular colors. We also offer MTS thiol-modifying and cross-linking agents.

We also offer many classic thiol-reactive dyes and biotin, as well as reducing agents commonly used in thiol conjugation protocols.

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    Product

    CF® Dye MTS

    From: $189 Sizes: 1 mgCatalog #:
    92096
    , 92097, 92098, - 92099View allHide

    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

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    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

     
    Product

    CF® Dye Maleimides

    From: $191 Sizes: 0.5 umol, 0.25 umol, 1 umolCatalog #:
    96069
    , 96079, 96074, 96062, 92020, 92021, 92022, 92023, 92024, 92025, 92026, 92027, 92028, 92029, 92030, 92031, 92032, 92033, 92034, 92044, 92045, 92046, 92118, 92124, 92128, 96012, 96015, - 96017View allHide

    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

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    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

     
    Product

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate

    From: $106 Sizes: 10 mgCatalog #: 91044

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     
    Product

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate

    From: $102 Sizes: 10 mgCatalog #: 91043

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     
    Product

    3,6-Dioxaoctane-1,8-diyl dimethanethiosulfonate

    From: $106 Sizes: 10 mgCatalog #: 91042

    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     
    Product

    ABD-F

    From: $95 Sizes: 10 mgCatalog #: 91046

    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

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    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

     
    Product

    AEAETS

    From: $126 Sizes: 100 mgCatalog #: 91031

    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

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    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

     
    Product

    Biotin TS Ethylenediamine

    From: $193 Sizes: 10 mgCatalog #:
    92010
    , 92011, - 92012View allHide

    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

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    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

     
    Product

    BisEA

    From: $135 Sizes: 50 mgCatalog #: 91045

    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

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    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

     
    Product

    Carboxyrhodamine 110-C6-Maleimide, 5 and 6 isomers

    From: $387 Sizes: 5 mgCatalog #: 91029

    CR110-C6-maleimide (full name: 5(6)-Carboxyrhodamine 110-C6-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110.

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    CR110-C6-maleimide (full name: 5(6)-Carboxyrhodamine 110-C6-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110.

     
    Product

    CFQ520 maleimide

    From: $266 Sizes: 5 mgCatalog #: 80502

    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

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    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

     
    Product

    CPM

    From: $195 Sizes: 25 mgCatalog #: 91010

    CPM

    CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

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    CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

     
    Product

    CPMTS

    From: $91 Sizes: 5 mgCatalog #: 91011

    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

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    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

     
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    DTT

    From: $38 Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     
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    Eosin-5-maleimide

    From: $264 Sizes: 25 mgCatalog #: 92013

    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

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    25 mg
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    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

     
    Product

    Fluorescein-5-maleimide

    From: $227 Sizes: 25 mgCatalog #: 91028

    Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.

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    Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.