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Thiol-Reactive Dyes

Maleimide reacts with thiol groups to form thioether-coupled products. The reaction can take place at pH 7 in the presence of amines. At neutral pH, the maleimide group does not react with histidine or arginine. Maleimide labeling can be a useful alternative to succinimidyl ester labeling in cases where modification of lysines alters antibody binding affinity or other biological activity of the target protein. We offer more than 20 bright and photostable CF® Dye Maleimides.

Methanethiosulfonate (MTS) is a fast-reacting and highly selective thiol-reactive functional group. We offer CF® Dye MTS with a selection of popular colors. We also offer MTS thiol-modifying and cross-linking agents.

We also offer many classic thiol-reactive dyes and biotin, as well as reducing agents commonly used in thiol conjugation protocols.

Showing 1–16 of 44 results

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate

    From: $91 Sizes: 10 mgCatalog #: 91044

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate

    From: $87 Sizes: 10 mgCatalog #: 91043

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    3,6-Dioxaoctane-1,8-diyl dimethanethiosulfonate

    From: $91 Sizes: 10 mgCatalog #: 91042

    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

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    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    ABD-F

    From: $74 Sizes: 10 mgCatalog #: 91046

    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

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    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

     

    AEAETS

    From: $109 Sizes: 100 mgCatalog #: 91031

    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

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    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

     

    Biotin ethylenediamine iodoacetamide

    From: $104 Sizes: 25 mgCatalog #: 90059

    Biotin ethylenediamine iodoacetamide is readily coupled to thiols.

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    Biotin ethylenediamine iodoacetamide is readily coupled to thiols.

     

    Biotin TS Ethylenediamine

    From: $166 Sizes: 10 mgCatalog #: 92010, 92011, - 92012View allHide

    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

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    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

     

    BisEA

    From: $116 Sizes: 50 mgCatalog #: 91045

    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

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    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

     

    Carboxyrhodamine 110-C5-Maleimide, 5 and 6 isomers

    From: $331 Sizes: 5 mgCatalog #: 91029

    CR110-C5-maleimide (full name: 5(6)-Carboxyrhodamine 110-C5-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110. The dye has spectral properties (λ Ex/λ Em (MeOH) = 502/524 nm) similar to those of fluorescein (fluorescein-5-maleimide, for example), but the photostability of the dye is among the best of all green fluorescent dyes.

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    CR110-C5-maleimide (full name: 5(6)-Carboxyrhodamine 110-C5-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110. The dye has spectral properties (λ Ex/λ Em (MeOH) = 502/524 nm) similar to those of fluorescein (fluorescein-5-maleimide, for example), but the photostability of the dye is among the best of all green fluorescent dyes.

     

    CF® Dye Maleimide

    From: $175 Sizes: 0.5 umol, 0.25 umol, 1 umolCatalog #: 96062, 92020, 92021, 92022, 92023, 92024, 92025, 92026, 92027, 92028, 92029, 92030, 92031, 92032, 92033, 92034, 92044, 92045, 92046, 92118, 92124, 92128, 96012, 96015, - 96017View allHide

    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups.

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    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups.

     

    CF® Dye MTS

    From: $168 Sizes: 1 mgCatalog #: 92096, 92097, 92098, - 92099View allHide

    MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.

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    MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.

     

    CFQ520 maleimide

    From: $236 Sizes: 5 mgCatalog #: 80502

    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

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    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

     

    CPM

    From: $168 Sizes: 25 mgCatalog #: 91010

    CPM

    CPM (full name: 7-diethylamino-3-(4′-maleimidylphenyl)-4-methylcoumarin) is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

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    CPM (full name: 7-diethylamino-3-(4′-maleimidylphenyl)-4-methylcoumarin) is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

     

    CPMTS

    From: $79 Sizes: 5 mgCatalog #: 91011

    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

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    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

     

    DTT

    From: $32 Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     

    Eosin-5-maleimide

    From: $226 Sizes: 25 mgCatalog #: 92013

    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

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    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.