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Thiol-Reactive Dyes

Maleimide reacts with thiol groups to form thioether-coupled products. The reaction can take place at pH 7 in the presence of amines. At neutral pH, the maleimide group does not react with histidine or arginine. Maleimide labeling can be a useful alternative to succinimidyl ester labeling in cases where modification of lysines alters antibody binding affinity or other biological activity of the target protein. We offer more than 20 bright and photostable CF® Dye Maleimides.

Methanethiosulfonate (MTS) is a fast-reacting and highly selective thiol-reactive functional group. We offer CF® Dye MTS with a selection of popular colors. We also offer MTS thiol-modifying and cross-linking agents.

We also offer many classic thiol-reactive dyes and biotin, as well as reducing agents commonly used in thiol conjugation protocols.

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    CF® Dye MTS

    From: $178 Sizes: 1 mgCatalog #: 92096, 92097, 92098, - 92099View allHide

    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

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    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

     

    CF® Dye Maleimides

    From: $180 Sizes: 0.5 umol, 0.25 umol, 1 umolCatalog #: 96079, 96074, 96062, 92020, 92021, 92022, 92023, 92024, 92025, 92026, 92027, 92028, 92029, 92030, 92031, 92032, 92033, 92034, 92044, 92045, 92046, 92118, 92124, 92128, 96012, 96015, - 96017View allHide

    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

     

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate

    Sizes: 10 mgCatalog #: 91044

    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate

    Sizes: 10 mgCatalog #: 91043

    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    3,6-Dioxaoctane-1,8-diyl dimethanethiosulfonate

    Sizes: 10 mgCatalog #: 91042

    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.

     

    ABD-F

    From: $84 Sizes: 10 mgCatalog #: 91046

    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

     

    AEAETS

    Sizes: 100 mgCatalog #: 91031

    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.

     

    Biotin TS Ethylenediamine

    Sizes: 10 mgCatalog #: 92010, 92011, - 92012View allHide

    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.

     

    BisEA

    Sizes: 50 mgCatalog #: 91045

    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.

     

    Carboxyrhodamine 110-C5-Maleimide, 5 and 6 isomers

    Sizes: 5 mgCatalog #: 91029

    CR110-C5-maleimide (full name: 5(6)-Carboxyrhodamine 110-C5-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110. The dye has spectral properties (λ ExEm (MeOH) = 502/524 nm) similar to those of fluorescein (fluorescein-5-maleimide, for example), but the photostability of the dye is among the best of all green fluorescent dyes.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CR110-C5-maleimide (full name: 5(6)-Carboxyrhodamine 110-C5-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110. The dye has spectral properties (λ ExEm (MeOH) = 502/524 nm) similar to those of fluorescein (fluorescein-5-maleimide, for example), but the photostability of the dye is among the best of all green fluorescent dyes.

     

    CFQ520 maleimide

    Sizes: 5 mgCatalog #: 80502

    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

     

    CPM

    Sizes: 25 mgCatalog #: 91010

    CPM

    CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

     

    CPMTS

    Sizes: 5 mgCatalog #: 91011

    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

     

    DTT

    Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     

    Eosin-5-maleimide

    Sizes: 25 mgCatalog #: 92013

    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

     

    Fluorescein-5-maleimide

    Sizes: 25 mgCatalog #: 91028

    Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 mg
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    Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.