Dyes for Mitochondria & Other Organelles
Biotium sells a range of dyes to stain organelles such as the mitochondria and lysosomes in cells. Many of the mitochondria dyes are dependent on mitochondrial membrane potential and can therefore be used to monitor membrane potential changes that occur during processes such as apoptosis.
MitoView™ Dyes stain the mitochondria in living cells. They come in 3 colors, including both membrane potential-dependent and -independent options. We also offer a wide selection of classical potential-dependent mitochondrial dyes such as JC-1, TMRE, TMRM.
LysoView™ Dyes stain the lysosomes in living cells. Available in 5 colors, plus a novel UV-inducible version.
LipidSpot™ stains lipid droplets, cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters.
TMRM
TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.
Oxazole Gold (SYBR® Gold), 10,000X in DMSO
Oxazole Gold, also known as SYBR® Gold, is the most sensitive fluorescent gel stain available for DNA and RNA.
LipidSpot™ Lipid Droplet Stains
70065-T, 70065, 70069-T, - 70069View allHide
LipidSpot™ dyes rapidly stain lipid droplets in live cells or fixed cells, with no wash step required. Available with green or red/far-red fluorescence.
Rhodamine 123
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.
Aquaphile™ JC-1
An improved and more soluble formulation of the JC-1 dye used for ratiometric detection of mitochondrial potential changes.
MitoView™ Fix 640
70082, - 70082-50ugView allHide
Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.
MitoView™ Mitochondrial Dyes
70070-T, 70070, 70054-T, 70054, 70055-T, 70055, 70075-50ug, 70075, 70068-T, - 70068View allHide
Fluorogenic mitochondrial stains for live cells that rapidly accumulate in mitochondria and can be imaged without washing.
Light-on LysoView™ 555
70060, - 70060-TView allHide
Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is activated by exposure to UV excitation.
TMRE, 2 mM in DMSO
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is used to quantify changes in mitochondrial membrane potential in live cells.
4-Di-1-ASP
4-Di-1-ASP is a styryl dye that stains mitochondria of live cells. It has also been used to stain glioma cells in living brain tissues.
JC-1 (chloride salt)
A fluorescent mitochondrial dye that can be used for ratiometric detection of mitochondrial potential changes.
JC-1 (iodide salt)
A fluorescent mitochondrial dye that can be used for ratiometric detection of mitochondrial potential changes.
Nonyl Acridine Orange (NAO)
NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.
DiIC1(5) (1,1′-dimethyl-3,3,3′,3′-tetramethylindodicarbocyanine, iodide)
The carbocyanine dye DiIC1(5) has been used to measure mitochondrial membrane potential in apoptotic cells. Loss of membrane potential in apoptotic cells is reflected by a decrease of fluorescence signal in the infrared channel.
TMRE
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.
Tetrabromorhodamine 123, bromide
Tetrabromorhodamine 123 can be used to generate singlet oxygen (1O2) in mitochondrial membranes (1,2). The dye has a quantum yield for singlet oxygen generation of 0.65-0.7 and is particularly toxic to carcinoma cells.