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NUCLEIC ACID GEL STAINS

The most sensitive red fluorescent DNA gel stain, proven safer than EtBr
A safer and more sensitive alternative to SYBR® Green I green fluorescent gel stains
Similar to our classic GelRed™ stain but made to stain DNA in PAGE gels, with high sensitivity
A novel visible blue DNA gel stain, extremely sensitive and great for cutting out bands for cloning
A blue LED light box for the visualization of GelGreen™ stained gels, or the activation of DNAzure® stained gels

 

GELRED™ & GELGREEN™

How Safe is Your Gel Stain?

A number of ethidium bromide (EtBr) alternatives are marketed as being safe. In fact, many so-called “safe” gel stains contain dyes that are well known to bind DNA in living cells, with cytotoxic effects. GelRed™ and GelGreen™ are highly sensitive gel stains designed to be nontoxic and nonmutagenic by virtue of being cell membrane impermeable, so they cannot enter living cells. Download our white paper to learn more.


GelRed™ and GelGreen™

Safer alternatives to EtBr, SYBR® Safe, and others

EtBr has been the predominant dye used for nucleic acid staining for decades because of its low price and generally sufficient sensitivity. However, EtBr is a highly mutagenic material. The safety hazard and costs associated with decontamination and waste disposal can ultimately make the nucleic acid dye expensive to use. The dye experts at Biotium developed the extremely popular GelRed™ and GelGreen™ fluorescent agarose gel stains to not only be the most sensitive DNA gel stains on the market, but also the safest: they are the only DNA gel stains that have been tested and found to be safe and non-toxic by independent laboratories.

To learn more, visit the GelRed™ and GelGreen™ technology page.

GelRed™ & GelGreen™ do not readily penetrate cells. HeLa cells were incubated for 30 minutes at 37°C with 1X SYBR® Safe, GelRed™, or GelGreen™. SYBR® Safe rapidly bound to DNA in live cells resulting in bright green nuclear staining. GelRed™ and GelGreen™ were unable to bind DNA in live cells, shown by the absence of fluorescence.

GelRed™ Features:

  • Visualized with UV transilluminators using ethidium bromide detection settings
  • Most sensitive fluorescent red DNA gel stain
  • Safer and more environmentally friendly than EtBr, as well as other so-called “safe” gel stains
  • Can be used as a post-stain, or precast in agarose
  • Available as a 10,000X stock, ready-to-use 3X solution, and in 6X loading buffer format
Comparison of ethidium bromide (EtBr) and GelRed in precast gel staining using 1% agarose gel in TBE buffer. Two-fold serial dilutions of 1 kb Plus DNA Ladder (Invitrogen) were loaded in the amounts of 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300 nm transilluminator and photographed with an EtBr filter.
Comparison of ethidium bromide (EtBr) and GelRed in precast gel staining using 1% agarose gel in TBE buffer. Two-fold serial dilutions of 1 kb Plus DNA Ladder (Invitrogen) were loaded in the amounts of 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300 nm transilluminator and photographed with an EtBr filter.

GelGreen™ Features:

  • Visualized with blue light boxes or UV light box using SYBR® green detection settings
  • More sensitive than SYBR® Safe
  • Safer for users and the environment than SYBR® Safe and other so-called “safe” gel stains
  • Can be used as a post-stain, or precast in agarose
  • Available in a concentrated 10,000X stock
Comparison of GelGreen and SYBR Safe in post-electrophoresis staining of 1% agarose/TBE gels. Two-fold serial dilutions (200 ng, 100 ng, 50 ng and 25 ng) of 1 kb Plus DNA Ladder (Invitrogen). Gels were imaged using 254-nm UV transilluminator and photographed with a SYBR filter.
Comparison of GelGreen and SYBR Safe in post-electrophoresis staining of 1% agarose/TBE gels. Two-fold serial dilutions (200 ng, 100 ng, 50 ng and 25 ng) of 1 kb Plus DNA Ladder (Invitrogen). Gels were imaged using 254-nm UV transilluminator and photographed with a SYBR filter.

Product NameCatalog NumberSize
GelRed™ 10,000X in water41003-T0.1 mL (Trial Size)
GelRed™ 10,000X in water410030.5 mL
GelRed™ 10,000X in water41003-110 mL
GelRed™ 3X in water410014 L
6X GelRed™ Prestain Loading Buffer, Blue Tracking Dye410091 mL
6X GelRed™ Prestain Loading Buffer, Orange Tracking Dye410101 mL
GelGreen™ 10,000X in Water410050.5 mL
GelGreen™ 10,000X in Water41005-110 mL

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PAGE GELRED™ FOR ACRYLAMIDE GELS


PAGE GelRed™ is a version of our popular GelRed™ dye that was specially formulated for staining DNA in polyacrylamide gels. Like the classic GelRed™, PAGE GelRed™ has also been found to be safe by independent laboratories.

Download the PAGE GelRed™ Safety Report.

PAGE GelRed™ Features:

  • Visualized with UV transilluminators using ethidium bromide detection settings
  • Formulated for efficient penetration and staining of polyacrylamide gels
  • Proven safe and environmentally friendly
  • Compatible with downstream cloning applications

Product NameCatalog NumberSize
PAGE GelRed™ 10,000X in Water41008-T0.1 mL
PAGE GelRed™ 10,000X in Water41008-500uL500 uL
PAGE GelRed™ 1X in Water410144 L

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DNAZURE® VISIBLE DNA GEL STAIN


As Biotium continues to innovate in gel staining, we now offer a truly unique visible DNA gel stain called DNAzure®. DNAzure® dye binds to DNA in an agarose gel, and after exposure to light develops visible blue bands. The blue staining is long-lasting and doesn’t require any further light source, making it ideal for cutting out bands (no worry about UV light damaging your DNA sample or your eyes). Even more amazing is that this visible stain is more sensitive than more fluorescent DNA gel stains.

DNAzure® Features:

  • Deep blue bands visible by the naked eye following 5-30 min light exposure
  • Ultrasensitive detection, as little as ~1 ng DNA
  • Simplified DNA band excision, without the need for DNA damaging UV light
  • Expensive gel documentation systems not required for imaging
  • Compatible with downstream cloning applications

Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel was stained with DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo-Plate™ Blue LED transilluminator. The gel was placed on a white light transilluminator and imaged with a cell phone camera.
Product NameCatalog NumberSize
DNAzure® Blue Nucleic Acid Gel Stain4102010 mL

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LED GEL ILLUMINATORS


Gel-Bright™ LED Gel Illuminator

The Gel-Bright™ LED Gel Illuminator was developed by Biotium as a simple, effective device for the visualization of fluorescent green DNA gel stains such as GelGreen™. It is also able to photoactivate the visible blue DNA gel stain, DNAzure®.

Gel-Bright™ Features:

  • Optimized for green dyes, such as GelGreen™ DNA Gel Stain
  • Compatible with red dyes such as GelRed™, though with reduced sensitivity
  • Can facilitate generation of visible blue DNA bands with DNAzure® visible DNA gel stain
  • Amber filter plate optimized for improved signal to background
  • Safer than a UV transilluminator; UV protective eyewear or clothing are not  required
  • No UV-induced damage of DNA
  • Blue LEDs angled diagonally for even illumination throughout the gel
Gel-Bright™ LED Gel Illuminator

Glo-Plate™ Blue LED Illuminator

The Glo-Plate™ Blue LED Gel Illuminator  is another blue LED device that is able to photoactivate the visible blue DNA gel stain, DNAzure®. It’s flat surface provides even illumination across the gel.

Glo-Plate™ Features:

  • Can facilitate generation of visible blue DNA bands with DNAzure® visible DNA gel stain
  • Safer than a UV transilluminator; UV protective eyewear or clothing are not  required
  • No UV-induced damage of DNA
  • Blue LEDs arranged under a diffuser plate allow even illumination from the bottom
  • 4 timer settings
  • Simple, lightweight design with a small footprint makes it easily portable
  • Also useful for photoactivation of PMA & PMAxx™ viability PCR dyes
Glo-Plate™ Blue LED Illuminator

Product NameCatalog NumberFunction
Gel-Bright™ LED Gel IlluminatorE90003Visualization of gels stained with fluorescent green dyes such as GelGreen™
Activation of the visible blue gel stain DNAzure®
Glo-Plate™ Blue LED IlluminatorE90004Activation of the visible blue gel stain DNAzure®
Activation of PMA & PMAxx™ viability PCR dyes

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GelRed Nucleic Acid Gel Stain

From: $26 Sizes: Trial size (0.1 mL), 0.5 mL, 10 mL, 4 LCatalog #: 41001, 41002, 41003, 41002-1, 41003-1, - 41003-TView allHide

PAGE GelRed Nucleic Acid Gel Stain

From: $22 Sizes: 0.1 mL, 500 uL, 4 LCatalog #: 41014, 41008-500uL, - 41008-TView allHide

GelGreen Nucleic Acid Gel Stain

From: $22 Sizes: Trial size (0.1 mL), 0.5 mL, 10 mLCatalog #: 41004, 41005, 41005-1, - 41005-TView allHide

Gel-Bright LED Gel Illuminator

From: $650 Sizes: EachCatalog #: E90003

DNAzure Blue Nucleic Acid Gel Stain, 100X

From: $120 Sizes: 10 mLCatalog #: 41020

Glo-Plate Blue LED Illuminator

From: $600 Sizes: EachCatalog #: E90004

Faq

GelRed™ and GelGreen™ troubleshooting

Many customers use GelRed™ or GelGreen™ precast gels for convenience. However, because GelRed™ and GelGreen™ are high affinity dyes designed to be larger dyes to improve their safety, they can affect the migration of DNA in precast gels. Some samples, such as restriction digested DNA may migrate abnormally in GelRed™ or GelGreen™ precast gels. Tip #1: Load less DNA Smearing and smiling in GelRed™ or GelGreen™ precast gels most often caused by overloading of DNA. If you see band migration shifts or smearing and smiling, try reducing the amount of DNA loaded. The recommended loading amount for ladders and samples of known concentration is 50-200 ng/lane. For samples of unknown concentration, try loading one half or one third of the usual amount of DNA. This usually solves band migration problems. Tip #2: Try the post-staining protocol To avoid any interference the dye may have on DNA migration, we recommend using the post-staining protocol. If your application requires loading more than the recommended amount of DNA, use the post-staining protocol. While we recommend post-staining gels for 30 minutes, you may be able see bands in as little as five minutes, depending on how much DNA is present. Post-staining solutions can be reused. See the GelRed™ Product Information Sheet or GelGreen™ Product Information Sheet for detailed protocols. Other tips to improve agarose gel resolution:
  • If you see DNA migration issues or smearing after post-staining with GelRed or GelGreen, then the problem is not caused by the nucleic acid dye. Avoid overfilling gel wells to prevent smearing of DNA down the surface of the gel.
  • Pour a lower percentage agarose gel. Higher molecular weight DNA separates better with a lower percentage gel.
  • Change the running buffer. TBE buffer has a higher buffering capacity than TAE buffer.

Category: GelRed™ and GelGreen™ troubleshooting, GelRed™ and GelGreen™ Nucleic Acid Gel Stains

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The dye may have precipitated out of solution.
  1. Heat GelRed™ solution to 45-50°C for two minutes and vortex to dissolve.
  2. Store dye at room temperature to avoid precipitation.

Category: GelRed™ and GelGreen™ troubleshooting, GelRed™ and GelGreen™ Nucleic Acid Gel Stains

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GelRed™ and GelGreen™ Nucleic Acid Gel Stains

The main difference between GelRed™ and GelGreen™ is their fluorescence excitation and emission wavelengths. GelRed™ has red fluorescence, similar to ethidium bromide. GelGreen™ has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen™ is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation. GelRed™ and GelGreen™ have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed™ is more sensitive for staining single stranded nucleic acids than GelGreen™. GelRed™ is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen™ for staining single stranded nucleic acids. For more information about these products, please visit our DNA stains technology page.

View the GelRed™ Product Line

View the GelGreen™ Product Line

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

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The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

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GelRed™ is compatible with a standard UV transilluminator (302 or 312 nm). GelGreen™ has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen™ is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

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DNAzure® visible DNA gel stain

For optimal staining, we recommend diluting the 100X DNAzure® stock to 1X working solution fresh each time. However, if a 1X solution stored at 4°C and protected from light, the gel stain should to be good up to two weeks.

Category: DNAzure® visible DNA gel stain

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Yes, the 1X DNAzure® staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

Category: DNAzure® visible DNA gel stain

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We recommend storing DNAzure® at 4°C. After storage at room temperature, we have seen some loss of dye stability.

Category: DNAzure® visible DNA gel stain

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