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EvaEZ™ Fluorometric Polymerase Activity Assay Kit

An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.

Product Attributes

Storage Conditions

Store at -10 to -35 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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2 x 1 mL
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Supporting Documents

Product Description

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.

  • Ideal for measuring Taq DNA polymerase activity
  • Can also be used for other DNA polymerases such as Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I
  • Kit contains EvaGreen® dye, a primed template, dNTPs, and MgCl2 in a Tris buffer system
  • Available without magnesium by special order

In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen® dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase. The activity assay can be conducted at temperatures from 4°C to 75°C.

EvaEZ™ Polymerase Activity Mix is provided at 2X concentration with 5 mM MgCl2 (final Mg2+ concentration in assay is 2.5 mM). Magnesium-free EvaEZ™ Polymerase Activity Mix (29051MF) is available on request for customers who wish to control the magnesium concentration in the assay. Contact customer service at to inquire about availability.

Phusion and SuperScript are registered trademarks of Thermo Fisher Scientific.


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3. PLoS One (2016) Jan 28;11(1):e0147234 doi: 10.1371/journal.pone.0147234
4. Appl Microbiol Biotechnol (2018) Jan;102(2):713-721 doi: 10.1007/s00253-017-8560-6
5. Electronic J Biotech (2020) 44; pp6-13 doi:10.1016/j.ejbt.2020.01.005


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