MIX-N-STAIN™ CF® DYE ANTIBODY LABELING TECHNOLOGY
Mix-n-Stain™ antibody labeling kits dramatically simplify the process of antibody labeling. The resulting conjugates perform comparably to pre-labeled antibodies from leading suppliers. Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging and multiplex flow cytometry.
Each Mix-n-Stain™ kit comes with everything you need to perform the conjugation. Without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.
One breakthrough feature of Mix-n-Stain™ kits is that antibody labeling can be conducted in the presence of many common buffer components such as BSA, gelatin, azide and low concentrations of Tris. As a result, the kits are compatible with most commercial antibody formulations without an antibody purification step prior to the conjugation process.
See below for our selection of Mix-n-Stain™ kits with a variety of label options.
Rapid Antibody Labeling with Next-Generation Fluorescent CF® Dyes, Biotin, & More
With Mix-n-Stain™ CF® Dye Antibody Labeling Kits, you can covalently label as little as ≤5 ug up to 100 ug of an antibody in just 30 minutes, with minimal hands-on time and 100% recovery of antibody. With slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience. See the Compatibility and Protocol Selection Flowchart below, or download the Product Protocol for a step-by-step worksheet to determine kit compatibility with your antibody.
These kits feature a choice of 29 superior CF® dyes, which have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, or other fluorescent dyes. Due to the high water solubility of CF® dyes, other proteins like BSA or gelatin in the labeling reaction have minimal effect on non-specific background, because non-IgG proteins are washed away during IF staining. We also offer Mix-n-Stain™ kits for biotin, FITC, DIG, and DNP labeling, as well as Mix-n-Stain™ Maxi Antibody Labeling Kits for 1 mg scale labeling reactions.
- 3 kit sizes: 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Choice of 29 CF® Dye colors, biotin, FITC, or haptens
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification of the product is required
- Compatible with common antibody stabilizers
Compatible with antibody stabilizers and ascites
Combine direct & indirect IF for flexible multiplexing
Featuring CF® Dyes with superior brightness
Mix-n-Stain CF® Dye, Biotin, FITC & Hapten Antibody Labeling Kits
|Product description||Label options||Labeling time|
|Mix-n-Stain™ CF® Dye Antibody Labeling Kits||Choice of 29 bright & photostable CF® Dyes||30 minutes (~30 seconds hands-on time)
Download the product protocol
|Mix-n-Stain™ Maxi Antibody Labeling Kits||Choice of 12 bright & photostable CF® Dyes, FITC, Cyanine 555 (Cy®3), and Cyanine 647 (Cy®5)|
|Mix-n-Stain™ Nanobody Labeling Kits||Choice of 7 bright & photostable CF® Dyes and Biotin|
|Mix-n-Stain™ FITC Antibody Labeling Kits||FITC|
|Mix-n-Stain™ Biotin Antibody Labeling Kits||Biotin|
|Mix-n-Stain™ Digoxigenin Antibody Labeling Kits||Digoxigenin (DIG)|
|Mix-n-Stain™ DNP Antibody Labeling Kits||DNP|
MIX-N-STAIN™ NANOBODY LABELING KITS
Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.
The kits come with a selection of our next-generation CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).
- Label 5-20 ug or 20-50 ug of Nanobody®
- Choice of 7 CF® Dye colors or biotin
- Simple 30 minute labeling, no purification
- Compatible with common stabilizers
- Dye options for super-resolution and 2-photon imaging (learn more)
Mix-n-Stain™ Nanobody Labeling Kits
|Label||Ex/Em||Labeling size |
|CF®405S||404/431 nm||5-20 ug||92502|
|CF®488A||490/515 nm||5-20 ug||92504|
|CF®568||562/583 nm||5-20 ug||92506|
|CF®594||593/614 nm||5-20 ug||92508|
|CF®640R||642/662 nm||5-20 ug||92510|
|CF®647||650/665 nm||5-20 ug||92512|
|CF®680R||680/701 nm||5-20 ug||92514|
MIX-N-STAIN™ PE, APC, PERCP, & TANDEM DYE ANTIBODY LABELING KITS
R-PE (commonly called PE), APC and PerCP are fluorescent proteins widely used in flow cytometry. These fluorophores belong to the family of phycobiliproteins, which are derived from cyanobacteria and eukaryotic algae. Because of their high extinction coefficients and quantum yields, they are the brightest flow cytometry dyes in their respective channels. They also can be coupled to small molecule dyes to form long Stokes shift tandem dyes, which expand multiplexing capability. Traditional protein-protein conjugation is complicated and time-consuming, but Mix-n-Stain™ kits greatly simplify the procedure, making it easy for any biologist to label their antibody for flow cytometry.
- Label 25-50 ug, 50-100 ug, or 1 mg Ab
- Labeling procedure of ~ 4 hours or less with no purification
- Choice of 7 bright fluorescent labels
- Novel RPE and APC tandem dyes for the RPE-Texas Red®, PE-Cy®5, and APC-Cy®7 channels
- Greatly simplifies antibody labeling for flow cytometry
Mix-n-Stain™ Fluorescent Protein Antibody Labeling KitsNote: Kits are not recommended for labeling IgM antibodies. Cy Dye is a registered trademark of GE Healthcare.
MIX-N-STAIN™ HRP, ALKALINE PHOSPHATASE, and GOX ANTIBODY LABELING KITS
Mix-n-Stain™ Enzyme Antibody Labeling Kits allow you to prepare antibody conjugates labeled with horseradish peroxidase (HRP), alkaline phosphatase (AP), or glucose oxidase (GOx) in about 2-3 hours. The resulting labeled antibodies are suitable for use in applications such as western blotting, ELISA and immunohistochemistry.
- Label with HRP, alkaline phosphatase (AP), or glucose oxidase (GOx)
- 2-3 hour labeling, with no purification
- Compatible with western blotting, ELISA and immunohistochemistry
Mix-n-Stain™ Enzyme Antibody Labeling Kits
|Product description||Catalog number||Reaction size||Product protocol||Labeling time|
|Mix-n-Stain™ HRP Antibody Labeling Kit||92300, 92451||10-20 ug antibody|
|~3.5 hours (few minutes hands-on time)|
|92301||25-50 ug antibody|
|92302||50-100 ug antibody|
|Mix-n-Stain™ Maxi HRP Antibody Labeling Kit||92437||1 mg antibody||
PI-Mix-n-Stain™ Maxi HRP
|Mix-n-Stain™ Alkaline Phosphatase Antibody Labeling Kit||92314||25-50 ug antibody|
|~2 hours (few minutes hands-on time)|
|92315||50-100 ug antibody|
|Mix-n-Stain™ Glucose Oxidase Antibody Labeling Kit||92312||25-50 ug antibody||
|92313||50-100 ug antibody|
MIX-N-STAIN™ CF® DYE SMALL LIGAND LABELING KITS
Mix-n-Stain™ CF® dye small ligand labeling kits are designed for rapid labeling of small (MW ~ 150 – 5,000) and relatively high affinity biological ligands (or substrates). Labeling takes about 30 minutes, without a final purification step. The ligands to be labeled must contain an aliphatic amine group that is not required for biological activity of the ligand. The amine group will form a covalent linkage with the reactive CF® dye provided in the kit. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands with an aliphatic amine. Many other small ligands are also possible candidates if they meet the criteria described above.
Simply mix your small molecule ligand with the reaction buffer and the optimally formulated dye provided, and incubate for 30 minutes, followed by a brief 5 minute quenching step. No reactive Mix-n-Stain™ dye is available at the end of labeling; therefore the conjugate is ready for cell staining and imaging without further purification. Mix-n-Stain™ labeled ligands perform comparably to commercially-available pre-labeled ligands (Figure 1), at a fraction of the cost.
For live cell imaging, the Mix-n-Stain™ small ligand kits are ideal to generate fluorescent chemical tags that provide smaller, brighter, and more versatile alternatives to fluorescent proteins. Labeling of various chemical tags with Mix-n-Stain™ kits yielded highly efficient and specific labeling of the target proteins both on surface and inside of living cells. The Mix-n-Stain™ small ligand kits have also been proven compatible with fixed cell imaging and gel-based assays.
- Choice of 10 CF® Dye colors for extracellular staining
- Choice of 5 CF® Dye colors for intracellular staining
- For labeling molecules with a MW of 150-5000, containing an inactive aliphatic amine
- Suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands
- 30 minutes total reaction time, no purification necessary
Mix-n-Stain™ Small Ligand Kits
|Product description||Label options||Labeling time|
|Mix-n-Stain™ CF® Dye Small Molecule Labeling Kits||Extracellular Staining: Choice of 10 bright and stable CF® Dyes||~30 minutes (minimal hands-on time)
Download the product protocol
|Intracellular Staining: Choice of 5 bright and stable CF® Dyes|
CF® DYE SE
PROTEIN LABELING KITS
CF® Dye SE Protein Labeling Kits allow you to label your antibody of interest with one of our many bright and photostable CF® Dyes. The difference is that while the Mix-n-Stain™ kits come optimized for consistent labeling of small amounts of antibody, the SE Protein Labeling Kits are designed for 3 labelings of up to 1 mg protein with removal of free dye after labeling. The SE Protein Labeling Kits allow the user to optimize the amount of dye and antibody and determine the degree of protein labeling, for optimization of labeling and antibody performance.
- Choice of 19 CF® Dye colors or biotin
- Reagents for 3 labeling reactions with purification
- Each reaction can label up to 1 mg of antibody or protein
- Includes instructions for determining degree of labeling
VIVOBRITE™ NEAR-IR ANTIBODY LABELING KITS FOR IN VIVO IMAGING
VivoBrite™ Antibody Labeling Kits use the same labeling technology as our SE Protein Labeling Kits. The kits include the same components as the SE Protein Labeling Kits, with the addition of a syringe and syringe filter to sterile filter the labeled protein, allowing it to be used for in vivo small animal studies. The VivoBrite™ kits are offered in 4 near-IR CF® Dye colors, which are useful for small animal in vivo imaging. Biotium’s near-IR fluroescent dyes are best in class for brightness, photostability, and water solubility.
- Choice of 4 near-IR CF® Dye colors
- Kit contains reagents for 3 labeling reactions
- Each reaction can label up to 1 mg of antibody
- Kit can also be used to label other proteins
- The resulting labeled protein is purified and sterile
Protein Labeling Kits
|Product description||Label options||Product protocol|
|CF® Dye SE Protein Labeling Kits||19 CF® Dye colors across the spectrum, plus biotin||PI-CF® Dye SE Protein Labeling Kits|
|VivoBrite™ Antibody Labeling Kits for Small Animal In Vivo Imaging||4 near-IR CF® Dyes||PI-VivoBrite™ Antibody Labeling Kits|
Mix-n-Stain™ Antibody Labeling Kits
CF® Dye Alternatives for Akoya Tyramides
|Akoya Tyramide||Ex/Em (nm)||Biotium Tyramide||Ex/Em (nm)||Biotium Cat. No.||Notes||CF® Dye Features|
|TSA Coumarin||402/443||CF®405S||404/431||92197||Much brighter & more |
|Opal™ Polaris 480||450/500||CF®430||426/498||96053||Recommended |
for abundant targets
|TSA Fluorescein||494/517||Fluorescein||492/514||96018||Direct replacement||See CF®488A for a brighter &
much more photostable alternative
TSA Cyanine 3
|550/570||Cyanine 555||555/565||96020||Direct replacement |
for Cyanine 3
|See CF®555 & CF®568 for brighter
& more photostable alternatives.
|CF®555||555/565||96021||Brighter & more |
|TSA Plus Cyanine 3.5||581/596||CF®583R||586/609||96085||Brighter & more |
|TSA Cyanine 5||648/667||CF®640R||642/662||92175||Brighter & more |
|CF®647||650/665||96022||Brighter alternative||CF®647 Features|
|CF®660R||663/682||92195||Less cross-talk with visible red dyes; |
bright & extremely photostable
|TSA Plus Cyanine 5.5|
|CF®680R||680/701||92196||Bright & extremely|
|Opal™ Polaris 780||750/770||CF®754||745/786||96090||Recommended |
for abundant targets
|Unique NIR dye for TSA|
|TSA Biotin||N/A||Biotin-XX||N/A||92176||Direct replacement|
|TSA Plus DNP||N/A||DNP||N/A||96019||Direct replacement|
|TSA Plus DIG||N/A||N/A||N/A||N/A||N/A|