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ANTIBODY & PROTEIN LABELING KITS

Efficient, easy-to-use kits for labeling antibodies and other proteins with our superior CF® dyes and other labels.
Jump to a section below to choose a kit that’s right for you:

Easy-to-use with minimal hands-on time, Mix-n-Stain™ kits require no purification after labeling
Antibody labeling for flow cytometry made easy
Make your own enzyme-coupled antibodies
Create fluorescent SNAP-tag®, CLIP-tag™, Halotag® & other ligands, no purification required
Larger scale kits, for optimized protein labeling & purification
Kits for labeling antibodies with our superior near-IR CF® Dyes, for in vivo small animal imaging

MIX-N-STAIN™ CF® DYE ANTIBODY LABELING TECHNOLOGY


Mix-n-Stain™ antibody labeling kits dramatically simplify the process of antibody labeling. The resulting conjugates perform comparably to pre-labeled antibodies from leading suppliers. Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging and multiplex flow cytometry.

Each Mix-n-Stain™ kit comes with everything you need to perform the conjugation. Without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.

One breakthrough feature of Mix-n-Stain™ kits is that antibody labeling can be conducted in the presence of many common buffer components such as BSA, gelatin, azide and low concentrations of Tris. As a result, the kits are compatible with most commercial antibody formulations without an antibody purification step prior to the conjugation process.

See below for our selection of Mix-n-Stain™ kits with a variety of label options.

Rapid Antibody Labeling with Next-Generation Fluorescent CF® Dyes, Biotin, & More

With Mix-n-Stain™ CF® Dye Antibody Labeling Kits, you can covalently label as little as ≤5 ug up to 100 ug of an antibody in just 30 minutes, with minimal hands-on time and 100% recovery of antibody. With slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience.

These kits feature a choice of 24 superior CF® dyes, which have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, or other fluorescent dyes. Due to the high water solubility of CF® dyes, other proteins like BSA or gelatin in the labeling reaction have minimal effect on non-specific background, because non-IgG proteins are washed away during IF staining. We also offer Mix-n-Stain™ kits for biotin, FITC, DIG, and DNP labeling.

Features:

  • 3 kit sizes: 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 24 CF® Dye colors, biotin, FITC, or haptens
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification of the product is required
  • Compatible with common antibody stabilizers


Compatible with antibody stabilizers and ascites

Monoclonal mouse anti-tubulin clone DM1A in ascites or purified in PBS with 1% BSA was labeled with CF®488A Mix-n-Stain™ using the modified protocol for total protein labeling. The Mix-n-Stain™-labeled antibodies performed comparably in immunofluorescence staining of HeLa cells as unlabeled antibody (in ascites) detected with secondary antibody.

Combine direct & indirect IF for flexible multiplexing

Combined direct and indirect immunofluorescence staining of rat testis with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat) (microtubules, green), CF®555 Mix-n-Stain™ labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (actin filaments, cyan). See our Tech Tip: Combined Direct & Indirect Immunofluorescence Using Primary Antibodies from the Same Host.

Featuring CF® Dyes with superior brightness

Mouse anti-transferrin receptor antibody from (endosome and plasma membrane marker) was labeled using Lightning-Link® Rapid DyLight® Conjugation Kits from Novus Biologicals (left) or Mix-n-Stain™ CF® Dye Antibody Labeling Kits (right) according to manufacturers’ instructions. The CF® dye conjugates show higher signal and more specific staining compared the DyLight®conjugates when imaged using the same settings, due to the superior brightness of CF® dyes. The insets show the same field of view imaged with a higher gain setting.

Mix-n-Stain™ CF® Dye, Biotin, FITC & Hapten Antibody Labeling Kits

Product descriptionLabel optionsLabeling time
Mix-n-Stain™ CF® Dye Antibody Labeling KitsChoice of 24 bright & photostable CF® Dyes30 minutes (~30 seconds hands-on time)

Download the product protocol
Mix-n-Stain™ FITC Antibody Labeling KitsFITC
Mix-n-Stain™ Biotin Antibody Labeling KitsBiotin
Mix-n-Stain™ Digoxigenin Antibody Labeling KitsDigoxigenin (DIG)
Mix-n-Stain™ DNP Antibody Labeling KitsDNP

 

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MIX-N-STAIN™ PE, APC, PERCP, & TANDEM DYE ANTIBODY LABELING KITS

R-PE (commonly called PE), APC and PerCP are fluorescent proteins widely used in flow cytometry. These fluorophores belong to the family of phycobiliproteins, which are derived from cyanobacteria and eukaryotic algae. Because of their high extinction coefficients and quantum yields, they are the brightest flow cytometry dyes in their respective channels. They also can be coupled to small molecule dyes to form long Stokes shift tandem dyes, which expand multiplexing capability. Traditional protein-protein conjugation is complicated and time-consuming, but Mix-n-Stain™ kits greatly simplify the procedure, making it easy for any biologist to label their antibody for flow cytometry.

Features:

  • Label 25-50 ug, 50-100 ug, or 1 mg Ab
  • Labeling procedure of ~ 4 hours or less with no purification
  • Choice of 5 bright fluorescent labels
  • Novel RPE-CF®647T & APC-CF®750T tandem dyes for PE-Cy®5/APC-Cy®7 channels
  • Greatly simplifies antibody labeling for flow cytometry

 

Anti-mouse secondary antibody was labeled with APC using either the Mix-n-Stain™ APC Labeling Kit, or a competitor’s APC labeling kit. The antibodies were used to detect mouse anti-CD3 staining of Jurkat cells. The Mix-n-Stain™ labeled antibody showed lower background without primary antibody (pink) and higher signal on CD3-labeled cells (blue).

Mix-n-Stain™ Fluorescent Protein Antibody Labeling Kits

Product descriptionExcitation/emissionCatalog numberReaction size Product protocolLabeling time
Mix-n-Stain™ R-PE (PE) Antibody Labeling Kit496, 546, 565/
578 nm
9229825-50 ug antibody
PI-Mix-n-Stain™ R-PE
~4 hours (~15 minutes hands-on time)
9229950-100 ug antibody
Mix-n-Stain™ APC Antibody Labeling Kit650/660 nm9230625-50 ug antibody
PI-Mix-n-Stain™ APC
9230750-100 ug antibody
Mix-n-Stain™ PerCP Antibody Labeling Kit482/677 nm9230825-50 ug antibody
PI-Mix-n-Stain™ PerCP
9230950-100 ug antibody
Mix-n-Stain™ RPE-CF®647T Antibody Labeling Kit496/665
(PE-Cy®5 channel)
9234025-50 ug antibody
PI-Mix-n-Stain™ RPE-CF®647
~2 hours (few minutes hands-on time)
9234150-100 ug antibody
923461 mg antibody
Mix-n-Stain™ APC-CF®750T Antibody Labeling Kit650/780 nm
(APC-Cy®7 channel)
9231025-50 ug antibody
PI-Mix-n-Stain™ APC-CF®750T
9231150-100 ug antibody
Cy Dye is a registered trademark of GE Healthcare.

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MIX-N-STAIN™ HRP, ALKALINE PHOSPHATASE, and GOX ANTIBODY LABELING KITS

Mix-n-Stain™ Enzyme Antibody Labeling Kits allow you to prepare antibody conjugates labeled with horseradish peroxidase (HRP), alkaline phosphatase (AP), or glucose oxidase (GOx) in about 2-3 hours. The resulting labeled antibodies are suitable for use in applications such as western blotting, ELISA and immunohistochemistry.

Features:

  • Reaction sizes: 25-50 ug or 50-100 ug Ab (plus 10-20 ug for HRP)
  • Label with HRP, alkaline phosphatase (AP), or glucose oxidase (GOx)
  • 2-3 hour labeling, with no purification
  • Compatible with western blotting, ELISA and immunohistochemistry

Mix-n-Stain™ Enzyme Antibody Labeling Kits

Product descriptionCatalog numberReaction size Product protocolLabeling time
Mix-n-Stain™ HRP Antibody Labeling Kit9230010-20 ug antibody
PI-Mix-n-Stain™ HRP
~3.5 hours (few minutes hands-on time)
9230125-50 ug antibody
9230250-100 ug antibody
Mix-n-Stain™ Alkaline Phosphatase Antibody Labeling Kit9231425-50 ug antibody
PI-Mix-n-Stain™ AP
~2 hours (few minutes hands-on time)
9231550-100 ug antibody
Mix-n-Stain™ Glucose Oxidase Antibody Labeling Kit9231225-50 ug antibody
PI-Mix-n-Stain™ GOx
9231350-100 ug antibody

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MIX-N-STAIN™ CF® DYE SMALL LIGAND LABELING KITS


Mix-n-Stain™ CF® dye small ligand labeling kits are designed for rapid labeling of small (MW ~ 150 – 5,000) and relatively high affinity biological ligands (or substrates). Labeling takes about 30 minutes, without a final purification step. The ligands to be labeled must contain an aliphatic amine group that is not required for biological activity of the ligand. The amine group will form a covalent linkage with the reactive CF® dye provided in the kit. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands with an aliphatic amine. Many other small ligands are also possible candidates if they meet the criteria described above.

Simply mix your small molecule ligand with the reaction buffer and the optimally formulated dye provided, and incubate for 30 minutes, followed by a brief 5 minute quenching step. No reactive Mix-n-Stain™ dye is available at the end of labeling; therefore the conjugate is ready for cell staining and imaging without further purification. Mix-n-Stain™ labeled ligands perform comparably to commercially-available pre-labeled ligands (Figure 1), at a fraction of the cost.

For live cell imaging, the Mix-n-Stain™ small ligand kits are ideal to generate fluorescent chemical tags that provide smaller, brighter, and more versatile alternatives to fluorescent proteins. Labeling of various chemical tags with Mix-n-Stain™ kits yielded highly efficient and specific labeling of the target proteins both on surface and inside of living cells. The Mix-n-Stain™ small ligand kits have also been proven compatible with fixed cell imaging and gel-based assays.

Features:

  • Choice of 10 CF® Dye colors for extracellular staining
  • Choice of 6 CF® Dye colors for intracellular staining
  • For labeling molecules with a MW of 150-5000, containing an inactive aliphatic amine
  • Suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands
  • 30 minutes total reaction time, no purification necessary
Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIPamine conjugated to green fluorescent CF®488A using Mix-n-Stain™ compared to (B) CLIP-surface 488 from New England BioLabs. Cell nuclei were stained blue with Hoechst 33342.
Figure 2. Three color imaging in live HeLa cells. Cell surface protein ADRβ2 was labeled using the CF®488A Mix-n-Stain™ kit via the SNAP-tag® (green). Mitochondrial protein Cox8A was labeled using the CF®540 Mix-n-Stain™ kit via the CLIP-tag™ (red). Cell nuclei were stained blue with Hoechst 33342.

Mix-n-Stain™ Small Ligand Kits

Product descriptionLabel optionsLabeling time
Mix-n-Stain™ CF® Dye Small Molecule Labeling KitsExtracellular Staining: Choice of 10 bright and stable CF® Dyes~30 minutes (minimal hands-on time)

Download the product protocol
Intracellular Staining: Choice of 6 bright and stable CF® Dyes

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CF® DYE SE
PROTEIN LABELING KITS

CF® Dye SE Protein Labeling Kits allow you to label your antibody of interest with one of our many bright and photostable CF® Dyes. The difference is that while the Mix-n-Stain™ kits come optimized for consistent labeling of small amounts of antibody, the SE Protein Labeling Kits are designed for 3 labelings of up to 1 mg protein with removal of free dye after labeling. The SE Protein Labeling Kits allow the user to optimize the amount of dye and antibody and determine the degree of protein labeling, for optimization of labeling and antibody performance.

Features:

  • Choice of 19 CF® Dye colors or biotin
  • Reagents for 3 labeling reactions with purification
  • Each reaction can label up to 1 mg of antibody or protein
  • Includes instructions for determining degree of labeling

VIVOBRITE™ NEAR-IR ANTIBODY LABELING KITS FOR IN VIVO IMAGING

VivoBrite™ Antibody Labeling Kits use the same labeling technology as our SE Protein Labeling Kits. The kits include the same components as the SE Protein Labeling Kits, with the addition of a syringe and syringe filter to sterile filter the labeled protein, allowing it to be used for in vivo small animal studies. The VivoBrite™ kits are offered in 4 near-IR CF® Dye colors, which are useful for small animal in vivo imaging. Biotium’s near-IR fluroescent dyes are best in class for brightness, photostability, and water solubility.

Features:

  • Choice of 4 near-IR CF® Dye colors
  • Kit contains reagents for 3 labeling reactions
  • Each reaction can label up to 1 mg of antibody
  • Kit can also be used to label other proteins
  • The resulting labeled protein is purified and sterile

Protein Labeling Kits

Product descriptionLabel optionsProduct protocol
CF® Dye SE Protein Labeling Kits19 CF® Dye colors across the spectrum, plus biotinPI-CF® Dye SE Protein Labeling Kits
VivoBrite™ Antibody Labeling Kits for Small Animal In Vivo Imaging4 near-IR CF® DyesPI-VivoBrite™ Antibody Labeling Kits

 

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Alexa Fluor is a registered trademark of Thermo Fisher Scientific. Cy is a registered trademark of GE Healthcare. SNAP-tag is a registered trademark of New England Biolabs. CLIP-tag is a trademark of New England Biolabs. HaloTag is a registered trademark of Promega.

Mix-n-Stain™ Biotin Antibody Labeling Kit

Sizes: 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labelingCatalog #: 92244, 92266, - 92286View allHide

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

From: $95 Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92230, 92230, 92231, 92232, 92233, 92234, 92235, 92236, 92237, 92238, 92239, 92240, 92241, 92242, 92243, 92245, 92246, 92247, 92248, 92250, 92250, 92251, 92252, 92253, 92254, 92255, 92256, 92257, 92258, 92259, 92260, 92261, 92262, 92263, 92264, 92265, 92267, 92268, 92270, 92270, 92271, 92272, 92273, 92274, 92275, 92276, 92277, 92278, 92279, 92280, 92281, 92282, 92283, 92284, 92285, 92287, 92288, 92289, 92290, 92291, 92303, 92304, 92305, 92316, 92317, 92318, 92319, 92320, 92321, 92322, 92323, 92324, 92331, 92332, 92333, 92334, 92335, 92336, 92337, 92338, - 92339View allHide

Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits

Sizes: 1 labelingCatalog #: 92350, 92351, 92352, 92353, 92354, 92355, 92356, 92357, 92358, 92359, 92360, 92361, 92362, 92363, - 92364View allHide

Mix-n-Stain™ Digoxigenin Antibody Labeling Kit

Sizes: 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labelingCatalog #: 92328, 92329, - 92330View allHide

Mix-n-Stain™ Enzyme Antibody Labeling Kits

Sizes: 25-50 ug labeling, 50-100 ug labelingCatalog #: 92300, 92301, 92302, 92312, 92313, 92314, - 92315View allHide

Mix-n-Stain™ FITC Antibody Labeling Kit

Sizes: 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labelingCatalog #: 92294, 92295, - 92296View allHide

Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits

From: $115 Sizes: 1 mg labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92298, 92299, 92306, 92307, 92308, 92309, 92310, 92311, 92340, 92341, - 92346View allHide

CF® Dye & Biotin SE Protein Labeling Kits

From: $383 Sizes: 3 labelingsCatalog #: 92208, 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide

VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging

From: $415 Sizes: 3 labelingsCatalog #: 92160, 92161, 92162, - 92163View allHide

Faq

Mix-n-Stain™ Antibody Labeling Kits

CF® dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF® dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF® dyes, the CF® Dye Selection Guide, and our CF® Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

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See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

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See the Mix-n-Stain™ Kit Selection Guide, below:
Antibody formulationKit and protocol selectionNotes/Examples
Purified IgG containing:

• Sodium azide

• Less than 20 mM Tris

• Less than 10% glycerol
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• More than 20 mM Tris

• More than 10% glycerol

• Glycine

• Less than 0.5 ug/uL IgG
1. Choose the kit size based on the amount of IgG you wish to label

2. Perform ultrafiltration using the spin vial provided in the kit

3. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• Less than 4:1 BSA:IgG by weight

• Less than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS contain­ing 0.1% BSA. The BSA:IgG ratio by weight is 5 ug BSA:5 ug IgG or 1:1. Select a 5-20 ug-sized kit and follow the standard protocol.
Purified IgG containing:

• More than 4:1 BSA:IgG by weight

• More than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the total amount of protein (IgG + BSA/gelatin) in the volume of antibody solution you wish to label

2. Use the modified labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS containing 1% BSA. The BSA:IgG ratio by weight is 50 ug BSA: 5 ug IgG or 10/1. Select a 50-100 ug-sized kit based on 55 ug of total protein in the labeling reaction and follow the modified labeling protocol. If the total protein amount falls between two kit sizes, you may get better results with the larger kit size.
IgG in ascites fluid1. Determine the concentration of protein in the ascites fluid

2. Choose the kit size based on the total amount of protein in the volume of ascites fluid you wish to label

3. Use the modified labeling protocol
For example, if you wish to label 10 uL ascites fluid containing 70 ug total protein. Select a 50-100 ug-sized kit based on 70 ug of total protein, and follow the modified labeling protocol.
Less than 5 ug purified IgG1. Add stabilizer protein such as BSA to the antibody to bring the total amount of protein 5 ug

2. Select a 5-20 ug kit and use the standard labeling protocol
For example, if you wish to label 1 ug IgG sample. Select a 5-20 ug-sized kit. Before labeling, add 4 ug BSA to 1 ug IgG and follow the standard labeling protocol.
IgG in:

• Serum

• Hybridoma cell culture supernatant
Not compatible due to the low concentration of IgG compared to other proteins in these formats; purify IgG before labelingUse protein A/G or a commercially available IgG clean-up kit to purify IgG. Determine the concentration of IgG, then select the appropriate kit/protocol based on the amount of IgG you wish to label and the buffer formulation after purification.

Category: Mix-n-Stain™ Antibody Labeling Kits

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CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

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CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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