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dsDNA QUANTITATION KITS

ACCUBLUE™ ACCUCLEAR™ & ACCUGREEN™

Formulated specifically for use with the Qubit® fluorometer from Thermo Fisher
Unrivaled sensitivity for ultra low amounts of DNA
Very high sensitivity and dynamic range
Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
Assay can be extended to 4000 ng dsDNA with minor loss of linearity

AccuBlue™, AccuClear™ and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers five dsDNA quantitiation kits for different instruments and sample concentration ranges.

To choose the DNA Quantitation Assay that is right for you, download our DNA Quantitation Kit Flyer, or view the detailed product highlights on this page.


Linear detection ranges of Biotium dsDNA Quantitation Assays

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher.
AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.

Product highlights

AccuBlue™ NextGen

  • Linear range: 1-3000 pg dsDNA
  • Green fluorescence (Ex/Em: 460/507 nm)
  • Unrivaled sensitivity for ultra low amounts of DNA
  • Assay stability: prepared working solution can be stored up to 6 months in the fridge for added convenience.
  • Novel green fluorescent dye is a perfect match for blue LED excitation sources
  • Ideal for fluorescence microplate reader

Standard curve of calf thymus DNA assayed using the AccuBlue NextGen Kit
Standard curve of calf thymus DNA assayed using the AccuBlue NextGen Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.


AccuClear™ Ultra High Sensitivity

  • Linear range: 0.03-250 ng dsDNA
  • Green fluorescence (Ex/Em: 468/507 nm)
  • Very high sensitivity and dynamic range
  • Novel green fluorescent dye is a perfect match for blue LED excitation sources
  • Compatible with fluorescence microplate reader or NanoDrop® fluorospectrometer

Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit
Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.


AccuBlue™ High Sensitivity

  • Linear range: 0.2-100 ng dsDNA
  • Green fluorescence (Ex/Em: 485/530 nm)
  • Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
  • Compatible with fluorescence microplate reader, NanoDrop® fluorospectrophotometer, and handheld fluorometers like Qubit® and QuantiFluor™-P

Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit
Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.


AccuGreen™ High Sensitivity (for Qubit®)

  • Linear range: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em: 502/523 nm)
  • Formulated specifically for use with the Qubit® fluorometer from Thermo Fisher


AccuBlue™ Broad Range

  • Blue fluorescence (Ex/Em: 350/460 nm)
  • Linear range: 2-2000 ng dsDNA
  • Assay can be extended to 4000 ng dsDNA with minor loss of linearity
  • Ideal for fluorescence microplate reader

Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits.
Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits. AccuBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range assay.


AccuBlue and AccuClear are covered by granted and pending US and international patents. AccuBlue, AccuClear and AccuGreen are trademarks of Biotium, Inc.; PicoGreen, Qubit and Quant-iT and NanoDrop are trademarks of Thermo Scientific.

AccuBlue Broad Range dsDNA Quantitation Kit with DNA Standards

From: $53 Sizes: 1500 assays, Trial sizeCatalog #: 31007-T, - 31007View allHide

AccuGreen High Sensitivity dsDNA Quantitation Kit

From: $75 Sizes: 100 assays, 500 assaysCatalog #: 31066, - 31066-TView allHide

AccuBlue High Sensitivity dsDNA Quantitation Kit with DNA Standards

From: $53 Sizes: Trial size, 1,000 assaysCatalog #: 31006, - 31006-TView allHide

AccuBlue NextGen dsDNA Quantitation Kit

From: $84 Sizes: Trial size, 1,000 assaysCatalog #: 31060, - 31060-TView allHide

Faq

DNA Quantitation Kits

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Please see the AccuBlue, AccuClear and AccuGreen Technology Highlights to choose the kit that best fits your application.

Category: DNA Quantitation Kits

← FAQs
The AccuClear™ and AccuBlue™ DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear™, AccuBlue™ NextGen and AccuBlue™ High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue™ Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument. The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

Category: DNA Quantitation Kits

← FAQs
For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue™ NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Also see our AccuClear™ Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA). If you own a Qubit® fluorometer, we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation kit, which is designed for use with the Qubit® fluorometer. It is a direct replacement for the Qubit® dsDNA HS Assay kit. Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

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