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dsDNA QUANTITATION KITS

ACCUBLUE®, ACCUCLEAR® & ACCUGREEN™

This ultra high sensitivity DNA quantitation assay can detect ultra low amounts of DNA better than any competitor kit on the market
A versatile, highly sensitive assay with an extremely broad dynamic range
AccuGreen™ High Sensitivity and Broad Range kits are affordable alternatives to Qubit® dsDNA kits
Wide linear range of 2-2000 ng
Non-mutagenic dye for safer handling & easy disposal

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. Some of our kits offer unrivaled sensitivity or linear ranges. To choose the DNA quantitation assay that is right for you, see the linear detection ranges and features table below.

Microplate reader assay features

  • Allow quantitation of many samples and replicates at once, for better accuracy
  • Microplate readers have the highest sensitivity and adjustable excitation and emission settings
  • The AccuBlue® NextGen assay is the most sensitive DNA quantitation assay on the market, allowing accurate detection of 1 pg* of DNA
  • The AccuClear® assay is extremely versatile, with a very broad dynamic range, allowing quantitation of nearly any sample

Qubit® reader assay features

  • AccuGreen™ assay kits designed for use in the pre-programmed dsDNA quantitation assays on the Qubit® reader
  • AccuGreen™ assay kits are direct replacements for the Qubit® assay kits
  • Significant cost savings when compared to the Qubit® kits

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

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ACCUBLUE® NEXTGEN


  • Linear range: 1-3000 pg dsDNA (depending on plate reader capability)
  • Unrivaled sensitivity for ultra low amounts of DNA
  • Working solution stable for up to 6 months in the fridge for added convenience
  • Green fluorescence (Ex/Em: 460/507 nm)
  • Dye is a perfect match for blue LED excitation sources
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus dsDNA standard

Click here to download an App Note on using the AccuBlue® NextGen assay on the BMG CLARIOStar® to quantify extremely low amounts of DNA.

Standard curve of calf thymus DNA assayed using the AccuBlue® NextGen Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.

AccuBlue® NextGen Products

ProductDNA StandardsCatalog No.Size
AccuBlue® NextGen dsDNA Quantitation Kit10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31060-TTrial size (200 assays)
310601000 assays
AccuBlue® NextGen dsDNA Quantitation SolutionNone, for use with DNA standard of your choice31061-TTrial size (200 assays)
310611000 assays

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ACCUCLEAR® ULTRA HIGH SENSITIVITY

  • Linear range: 0.03-250 ng dsDNA
  • Very high sensitivity and dynamic range
  • Green fluorescence (Ex/Em: 468/507 nm)
  • Dye is a perfect match for blue LED excitation sources
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor™-P, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus DNA standards
Standard curve of calf thymus DNA assayed using the AccuClear® Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507 nm). Inset shows the lower end of the titration.

AccuClear® Products

ProductDNA StandardsCatalog No.Size
AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31028-TTrial size (200 assays)
Set of 7 prediluted calf thymus dsDNA standards310281000 assays
25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution310292000 assays
AccuClear® Ultra High Sensitivity dsDNA SolutionNone, for use with DNA standard of your choice31027-TTrial size (200 assays)
310271000 assays

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ACCUBLUE® HIGH SENSITIVITY

  • Linear range: 0.2-100 ng dsDNA
  • Non-toxic, non-mutagenic dye for safer handling and easy disposal
  • Green fluorescence (Ex/Em: 500/530 nm)
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus dsDNA standards
  • Set of standards also available separately
Standard curve of calf thymus DNA assayed using the AccuBlue® High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.

AccuBlue® High Sensitivity Products

ProductDNA StandardsCatalog No.Size
AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31006-TTrial size (200 assays)
Set of 8 prediluted calf thymus dsDNA standards310061000 assays
AccuBlue® High Sensitivity dsDNA SolutionNone, for use with DNA standard of your choice31008-TTrial size (200 assays)
310081000 assays
AccuBlue® High Sensitivity dsDNA Standards, Set of EightStand-alone set of standards (0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL) 31006C0.5 mL each

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ACCUGREEN™ KITS FOR QUBIT®

AccuGreen™ High Sensitivity

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em: 502/523 nm)
  • Replaces Qubit® dsDNA HS Assay Kit for cost savings

AccuGreen™ Broad Range

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 2-1000 ng dsDNA
  • Green fluorescence (Ex/Em: 500/530 nm)
  • Replaces Qubit® dsDNA BR Assay Kit for cost savings

AccuGreen™ Products

ProductDNA StandardsCatalog No.Size
AccuGreen™ High Sensitivity dsDNA Quantitation Kit (for Qubit®)0 & 10 ng/uL calf thymus dsDNA31066-TTrial size (100 assays)
31066500 assays
AccuGreen™ High Sensitvity dsDNA Quantitation Solution (for Qubit®)None, for use with DNA standard of your choice31068-TTrial size (100 assays)
31068500 assays
AccuGreen™ Broad Range dsDNA Quantitation Kit (for Qubit®)0 & 100 ng/uL calf thymus dsDNA31069-TTrial size (100 assays)
31069500 assays
AccuGreen™ Broad Range dsDNA Quantitation Solution (for Qubit®)None, for use with DNA standard of your choice31070-TTrial size (100 assays)
31070500 assays

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ACCUBLUE® BROAD RANGE

  • Linear range: 2-2000 ng dsDNA
  • Blue fluorescence emission (Ex/Em: 350/460 nm)
  • For fluorescence microplate reader
  • Available with or without calf thymus dsDNA standards
  • Set of standards also available separately
Standard curve of calf thymus DNA assayed using AccuBlue® Broad Range Kit and read on a microplate reader (Ex/Em 350/460). Inset shows the lower end of the titration.

AccuBlue® Broad Range Products

ProductDNA StandardsCatalog No.Size
AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards200 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31007-TTrial size (200 assays)
Set of 9 prediluted calf thymus dsDNA standards310071000 assays
AccuBlue® Broad Range dsDNA SolutionNone, for use with DNA standard of your choice31009-TTrial size (200 assays)
310091000 assays
AccuBlue® Broad Range dsDNA Standards, Set of NineStand-alone set of standards, 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL31007C0.5 mL each

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AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit, Quant-iT, PicoGreen and NanoDrop are trademarks of Thermo Fisher Scientific.

AccuBlue® NextGen dsDNA Quantitation Kit

From: $84 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31060, - 31060-TView allHide

AccuBlue® NextGen dsDNA Quantitation Solution

From: $58 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31061, - 31061-TView allHide

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards

From: $63 Sizes: Trial size (200 assays), 1,000 assays, 2,000 assaysCatalog #: 31028, 31028-T, - 31029View allHide

AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution

From: $53 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31027, - 31027-TView allHide

AccuGreen™ High Sensitivity dsDNA Quantitation Kit

From: $75 Sizes: 100 assays, 500 assaysCatalog #: 31066, - 31066-TView allHide

AccuGreen™ High Sensitivity dsDNA Quantitation Solution

From: $60 Sizes: 100 assays, 500 assaysCatalog #: 31068, - 31068-TView allHide

AccuGreen™ Broad Range dsDNA Quantitation Kit

From: $69 Sizes: 100 assays, 500 assaysCatalog #: 31069-T, - 31069View allHide

AccuGreen™ Broad Range dsDNA Quantitation Solution

From: $55 Sizes: 100 assays, 500 assaysCatalog #: 31070, - 31070-TView allHide

AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards

From: $53 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31006, - 31006-TView allHide

AccuBlue® High Sensitivity dsDNA Quantitation Solution

From: $42 Sizes: Trial size, 1,000 assaysCatalog #: 31008, - 31008-TView allHide

AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards

From: $53 Sizes: 1500 assays, Trial size (200 assays)Catalog #: 31007-T, - 31007View allHide

AccuBlue® Broad Range dsDNA Quantitation Solution

From: $42 Sizes: 1500 assays, Trial size (200 assays)Catalog #: 31009, - 31009-TView allHide

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

From: $79 Sizes: 0.5 mL eachCatalog #: 31006C

AccuBlue® Broad Range dsDNA Standards, Set of Nine

From: $79 Sizes: 0.5 mL eachCatalog #: 31007C

Faq

DNA Quantitation Kits

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

Category: DNA Quantitation Kits

← FAQs
The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument. The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit. The AccuGreen™ Broad Range DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA Broad Range assay, and is a replacement for the Qubit® dsDNA BR Assay kit.

Category: DNA Quantitation Kits

← FAQs
For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA). If you own a Qubit® fluorometer, we recommend our AccuGreen™ kits, which were designed for use with the Qubit® fluorometer.  AccuGreen™ High Sensitivity dsDNA Quantitation kit is a direct replacement for the Qubit® dsDNA HS Assay kit. AccuGreen™ Broad Range dsDNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay kit. Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

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