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AccuBlue™, AccuClear™ & AccuGreen™ dsDNA Quantitation Kits

AccuBlue™, AccuClear™ and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers five dsDNA quantitiation kits for different instruments and sample concentration ranges.

To choose the DNA Quantitation Assay that is right for you, download our DNA Quantitation Kit Flyer, or view the detailed product highlights on this page.

DNquantranges

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit fluorometer from Thermo Fisher.

PRODUCT HIGHLIGHTS

AccuGreen™ High Sensitivity (for Qubit®)

  • Linear range: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em: 502/523 nm)
  • Formulated specifically for use with the Qubit® fluorometer from Thermo Fisher

AccuBlue™ NextGen

  • Linear range: 1-3000 pg dsDNA
  • Green fluorescence (Ex/Em: 460/507 nm)
  • Unrivaled sensitivity for ultra low amounts of DNA
  • Assay stability: prepared working solution can be stored up to 6 months in the fridge for added convenience.
  • Novel green fluorescent dye is a perfect match for blue LED excitation sources
  • Ideal for fluorescence microplate reader
AccuBlueNextGen full range with low range overlay
Standard curve of calf thymus DNA assayed using the AccuBlue NextGen Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.

AccuClear™ Ultra High Sensitivity

  • Linear range: 0.03-250 ng dsDNA
  • Green fluorescence (Ex/Em: 468/507 nm)
  • Very high sensitivity and dynamic range
  • Novel green fluorescent dye is a perfect match for blue LED excitation sources
  • Compatible with fluorescence microplate reader or NanoDrop® fluorospectrometer

Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.
Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.

AccuBlue™ High Sensitivity

  • Linear range: 0.2-100 ng dsDNA
  • Green fluorescence (Ex/Em: 485/530 nm)
  • Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
  • Compatible with fluorescence microplate reader, NanoDrop® fluorospectrophotometer, and handheld fluorometers like Qubit® and QuantiFluor™-P

Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.
Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.

AccuBlue™ Broad Range

  • Blue fluorescence (Ex/Em: 350/460 nm)
  • Linear range: 2-2000 ng dsDNA
  • Assay can be extended to 4000 ng dsDNA with minor loss of linearity
  • Ideal for fluorescence microplate reader

Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits. AccuBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range assay.
Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits. AccuBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range assay.

 

AccuBlue and AccuClear are covered by granted and pending US and international patents. AccuBlue, AccuClear and AccuGreen are trademarks of Biotium, Inc.; PicoGreen, Qubit and Quant-iT and NanoDrop are trademarks of Thermo Scientific.