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DNA & RNA QUANTITATION KITS

ACCUBLUE®, ACCUCLEAR® & ACCUGREEN™

This ultra high sensitivity DNA quantitation assay can detect ultra low amounts of DNA better than any competitor kit on the market
A versatile, highly sensitive assay with an extremely broad dynamic range
AccuGreen™ High Sensitivity and Broad Range kits are affordable alternatives to Qubit® dsDNA kits
Wide linear range of 2-2000 ng
Non-mutagenic dye for safer handling & easy disposal
RNA-selective, sensitive assay for plate reader or Qubit®. The widest dynamic range, equal to Quant-iT™ HS and BR combined

Fluorescence-Based Nucleic Acid Quantitation

Fluorescence-based nucleic acid quantitation assays allow precise quantitation of purified dsDNA or RNA samples across a wide range of concentrations and a variety of fluorescence detection instruments.

Unlike absorbance-based nucleic acid quantitation, which cannot distinguish between DNA, RNA, and free nucleotides, fluorescent assays are highly selective for either dsDNA or RNA. Fluorescent assays also allow more sensitive detection of low concentrations of DNA or RNA, allowing you to preserve your precious samples.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. Some of our kits offer unrivaled sensitivity or linear ranges.

Biotium also offers an RNA quantitation kit with the widest linear range on the market and multi-instrument capability, making it the most convenient assay you will find.

Microplate reader dsDNA assay features

  • Allow quantitation of many samples and replicates at once, for better accuracy
  • Microplate readers have the highest sensitivity and adjustable excitation and emission settings
  • The AccuBlue® NextGen assay is the most sensitive DNA quantitation assay on the market, allowing accurate detection of 1 pg* of DNA
  • The AccuClear® assay is extremely versatile, with a very broad dynamic range, allowing quantitation of nearly any sample

Microplate reader dsDNA assay linear ranges

Qubit® reader dsDNA assay features

  • AccuGreen™ assay kits are designed for use in the pre-programmed dsDNA quantitation assays on the Qubit® reader
  • AccuGreen™ assay kits are direct replacements for the Qubit® assay kits
  • Significant cost savings when compared to the Qubit® kits

Qubit® reader dsDNA assay linear ranges

RNA assay features

  • Linear range (5-1000 ng RNA) spans both Qubit® HS & BR ranges
  • Highly selective for RNA over dsDNA
  • Far-red fluorescence emission
  • For fluorescence microplate reader or Qubit® fluorometer (RNA Broad Range program)
  • Mammalian RNA standard to accurately quantify similar samples

RNA assay linear range

All DNA & RNA Quantitation Kits

Kit NameDNA or RNADye Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity DNADNA502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNADNA468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA650/670Fluorescent Plate Reader or Qubit® fluorometerThe widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity

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ACCUBLUE® NEXTGEN DNA QUANTITATION


  • Linear range: 1-3000 pg dsDNA (depending on plate reader capability)
  • Unrivaled sensitivity for ultra low amounts of DNA
  • Working solution stable for up to 6 months in the fridge for added convenience
  • Green fluorescence (Ex/Em: 460/507 nm)
  • Dye is a perfect match for blue LED excitation sources
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus dsDNA standard

Click here to download an App Note on using the AccuBlue® NextGen assay on the BMG CLARIOStar® to quantify extremely low amounts of DNA.

Standard curve of calf thymus DNA assayed using the AccuBlue® NextGen Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.

AccuBlue® NextGen Products

ProductDNA StandardsCatalog No.Size
AccuBlue® NextGen dsDNA Quantitation Kit10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31060-TTrial size (200 assays)
310601000 assays
AccuBlue® NextGen dsDNA Quantitation SolutionNone, for use with DNA standard of your choice31061-TTrial size (200 assays)
310611000 assays

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ACCUCLEAR® ULTRA HIGH SENSITIVITY DNA QUANTITATION

  • Linear range: 0.03-250 ng dsDNA
  • Very high sensitivity and dynamic range
  • Green fluorescence (Ex/Em: 468/507 nm)
  • Dye is a perfect match for blue LED excitation sources
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor™-P, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus DNA standards
Standard curve of calf thymus DNA assayed using the AccuClear® Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507 nm). Inset shows the lower end of the titration.

AccuClear® Products

ProductDNA StandardsCatalog No.Size
AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31028-TTrial size (200 assays)
Set of 7 prediluted calf thymus dsDNA standards310281000 assays
25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution310292000 assays
AccuClear® Ultra High Sensitivity dsDNA SolutionNone, for use with DNA standard of your choice31027-TTrial size (200 assays)
310271000 assays
AccuClear® dsDNA Standard, 25 ng/uLStandalone DNA standard, 25 ng/uL31029C1 mL

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ACCUBLUE® HIGH SENSITIVITY DNA QUANTITATION

  • Linear range: 0.2-100 ng dsDNA
  • Non-toxic, non-mutagenic dye for safer handling and easy disposal
  • Green fluorescence (Ex/Em: 500/530 nm)
  • Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
  • Available with or without calf thymus dsDNA standards
  • Set of standards also available separately
Standard curve of calf thymus DNA assayed using the AccuBlue® High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.

AccuBlue® High Sensitivity Products

ProductDNA StandardsCatalog No.Size
AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31006-TTrial size (200 assays)
Set of 8 prediluted calf thymus dsDNA standards310061000 assays
AccuBlue® High Sensitivity dsDNA SolutionNone, for use with DNA standard of your choice31008-TTrial size (200 assays)
310081000 assays
AccuBlue® High Sensitivity dsDNA Standards, Set of EightStand-alone set of standards (0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL) 31006C0.5 mL each

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ACCUGREEN™ DNA QUANTITATION KITS FOR QUBIT®

AccuGreen™ High Sensitivity

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em: 502/523 nm)
  • Replaces Qubit® dsDNA HS Assay Kit for cost savings
A genomic DNA sample was quantified using both the Qubit® HS kit and the AccuGreen™ HS kit. A nearly identical result was obtained with each kit.

AccuGreen™ Broad Range

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 2-1000 ng dsDNA
  • Green fluorescence (Ex/Em: 500/530 nm)
  • Replaces Qubit® dsDNA BR Assay Kit for cost savings
A genomic DNA sample was quantified using both the Qubit® BR kit and the AccuGreen™ BR kit. A nearly identical result was obtained with each kit.

AccuGreen™ Products

ProductDNA StandardsCatalog No.Size
AccuGreen™ High Sensitivity dsDNA Quantitation Kit (for Qubit®)0 & 10 ng/uL calf thymus dsDNA31066-TTrial size (100 assays)
31066500 assays
AccuGreen™ High Sensitvity dsDNA Quantitation Solution (for Qubit®)None, for use with DNA standard of your choice31068-TTrial size (100 assays)
31068500 assays
AccuGreen™ Standard 2, 10 ng/uLStandalone DNA standard, 10 ng/uL99820-T1 mL
AccuGreen™ Broad Range dsDNA Quantitation Kit (for Qubit®)0 & 100 ng/uL calf thymus dsDNA31069-TTrial size (100 assays)
31069500 assays
AccuGreen™ Broad Range dsDNA Quantitation Solution (for Qubit®)None, for use with DNA standard of your choice31070-TTrial size (100 assays)
31070500 assays
AccuGreen™ Standard 2, 100 ng/uLStandalone DNA standard, 100 ng/uL99838-T1 mL

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ACCUBLUE® BROAD RANGE DNA QUANTITATION

  • Linear range: 2-2000 ng dsDNA
  • Blue fluorescence emission (Ex/Em: 350/460 nm)
  • For fluorescence microplate reader
  • Available with or without calf thymus dsDNA standards
  • Set of standards also available separately
Standard curve of calf thymus DNA assayed using AccuBlue® Broad Range Kit and read on a microplate reader (Ex/Em 350/460). Inset shows the lower end of the titration.

AccuBlue® Broad Range DNA Quantitation Products

ProductDNA StandardsCatalog No.Size
AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards200 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution31007-TTrial size (200 assays)
Set of 9 prediluted calf thymus dsDNA standards310071000 assays
AccuBlue® Broad Range dsDNA SolutionNone, for use with DNA standard of your choice31009-TTrial size (200 assays)
310091000 assays
AccuBlue® Broad Range dsDNA Standards, Set of NineStand-alone set of standards, 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL31007C0.5 mL each

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ACCUBLUE® RNA QUANTITATION KIT

AccuBlue® RNA Quantitation Kit Details

  • Linear range of 5-1000 ng RNA
  • Far-red fluorescence emission (Ex/Em: 650/670 nm)
  • Optimized for fluorescence microplate readers with far-red fluorescence
  • Optimized for the Qubit® 3 pre-programmed RNA BR program
  • Comes with a mammalian-derived total RNA standard

AccuBlue® RNA Quantitation Kit Advantages

  • Selective: Can tolerate equivalent amounts of dsDNA (see Figure below)
  • Convenient: Wide linear range spans Qubit®/Quant-iT™ HS and BR ranges
  • Flexible: For fluorescence microplate reader OR small fluorometer like Qubit®
  • Accurate: The mammalian-derived total RNA standard provides the highest accuracy when quantifying samples from similar sources
The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays.
The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. This figure shows that ti can tolerate an equal amount of dsDNA without affecting the RNA quantitation.

RNA Quantitation Kits

ProductCatalog No.Size
AccuBlue® Broad Range RNA Quantitation Kit
31073-TTrial size (200 assays)
AccuBlue® Broad Range RNA Quantitation Kit310731000 assays

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AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit, Quant-iT, PicoGreen and NanoDrop are trademarks of Thermo Fisher Scientific.

AccuBlue® NextGen dsDNA Quantitation Kit

From: $87 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31060, - 31060-TView allHide

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards

From: $69 Sizes: Trial size (200 assays), 1,000 assays, 2,000 assaysCatalog #: 31028, 31028-T, - 31029View allHide

AccuGreen™ High Sensitivity dsDNA Quantitation Kit

From: $77 Sizes: 100 assays, 500 assaysCatalog #: 31066, - 31066-TView allHide

AccuGreen™ Broad Range dsDNA Quantitation Kit

Sizes: 100 assays, 500 assaysCatalog #: 31069-T, - 31069View allHide

AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards

From: $55 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31006, - 31006-TView allHide

AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards

From: $55 Sizes: 1500 assays, Trial size (200 assays)Catalog #: 31007-T, - 31007View allHide

AccuBlue® Broad Range RNA Quantitation Kit

Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31073-T, - 31073View allHide

Faq

DNA Quantitation Kits

Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: CF® Dyes, DNA Quantitation Kits, Luciferase Assays, Mix-n-Stain™ Antibody Labeling Kits, NucView® Caspase-3 Enzyme Substrates, Product shipping, storage, shelf life, & solubility

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Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.  

Category: Apoptosis, Necrosis, and Cell Viability Kits, CF® Dyes, DNA Quantitation Kits, GelRed® and GelGreen® Nucleic Acid Gel Stains, Luciferase Assays, Mix-n-Stain™ Antibody Labeling Kits, NucView® Caspase-3 Enzyme Substrates, Product shipping, storage, shelf life, & solubility

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Bioscience kits The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples. Antibodies and other conjugates The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). Chemicals, dyes, and gel stains Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage. If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples. Expiration date based on date of manufacture (DOM) If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance. Chemical products with special stability considerations: Esters Ester compounds include the following: • Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes • Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes • Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound. A note on CF™ dye succinimidyl ester stability Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes. Maleimides, MTS and thiosulfate dyes Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions. Other reactive dyes Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information. Coelenterazines and D-luciferin Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution. Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution. Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced. D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: CF® Dyes, DNA Quantitation Kits, Luciferase Assays, Mix-n-Stain™ Antibody Labeling Kits, NucView® Caspase-3 Enzyme Substrates, Product shipping, storage, shelf life, & solubility

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