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Tyramides and Tyramide Signal Amplification Kits

Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger signal compared to conventional immunofluorescence. Learn more about tyramide signal amplification.

Biotium’s Tyramide Amplification Kits feature our bright and photostable CF® dyes or biotin with anti-mouse, anti-rabbit, or HRP streptavidin conjugates. The kits offer benefits of high sensitivity and signal-to-background ratio, enabling the visualization of low-abundance targets that cannot be efficiently labeled using traditional immunostaining methods. CF® dye, biotin, and other tyramides are also available separately.

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    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33000, 33003, 33006, 33009, 33012, 33015, - 33018View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    1 kit
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    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33001, 33004, 33007, 33010, 33013, 33016, - 33019View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    1 kit
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33002, 33005, 33008, 33011, 33014, 33017, - 33020View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    1 kit
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    CF® Dye Tyramide

    From: $275 Sizes: 0.5 mgCatalog #: 96077, 96066, 92170, 92171, 92172, 92173, 92174, 92175, 92194, 92195, 92196, 92197, 92198, 92199, 96021, 96022, 96052, 96053, - 96057View allHide

    Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    0.5 mg
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    Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

     

    Ready-to-Use Tyramide Amplification Buffer, 1X

    Sizes: 25 mL, 100 mLCatalog #: 22027-25mL, - 22027-100mLView allHide

    For simple, optimized tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    25 mL
    100 mL
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    For simple, optimized tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

     

    Tyramide Amplification Buffer Plus

    From: $110 Sizes: 250 reactions, 1000 reactionsCatalog #: 22029, - 22029-TView allHide

    An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    250 reactions
    1000 reactions
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    An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

     

    Biotin-XX Tyramide

    From: $271 Sizes: 0.5 mgCatalog #: 92176

    Biotin-XX tyramide conjugate can be used for tyramide signal amplification (TSA) to generate high-density labeling of a target protein or nucleic acid with biotin.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    0.5 mg
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    Biotin-XX tyramide conjugate can be used for tyramide signal amplification (TSA) to generate high-density labeling of a target protein or nucleic acid with biotin.

     

    Cyanine 555 Tyramide

    From: $289 Sizes: 0.5 mgCatalog #: 96020

    Cyanine 555  tyramide is used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    0.5 mg
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    (Please select quantity above )

    Cyanine 555  tyramide is used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

     

    DNP Tyramide

    From: $276 Sizes: 0.5 mgCatalog #: 96019

    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    0.5 mg
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    (Please select quantity above )

    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

     

    Fluorescein Tyramide

    From: $268 Sizes: 0.5 mgCatalog #: 96018

    Fluorescein tyramide can be used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
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    ADD TO CART
    (Please select quantity above )

    Fluorescein tyramide can be used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).