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Tyramides and Tyramide Signal Amplification Kits

Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger signal compared to conventional immunofluorescence. Learn more about tyramide signal amplification.

Biotium’s Tyramide Amplification Kits feature our bright and photostable CF® dyes or biotin with anti-mouse, anti-rabbit, or HRP streptavidin conjugates. The kits offer benefits of high sensitivity and signal-to-background ratio, enabling the visualization of low-abundance targets that cannot be efficiently labeled using traditional immunostaining methods. CF® dye, biotin, and other tyramides are also available separately.

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    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33000, 33003, 33006, 33009, 33012, 33015, - 33018View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

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    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33001, 33004, 33007, 33010, 33013, 33016, - 33019View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 kit
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    ADD TO CART
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    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33002, 33005, 33008, 33011, 33014, 33017, - 33020View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 kit
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    CF® Dye Tyramides

    Sizes: 0.5 mgCatalog #: 96066, 92170, 92171, 92172, 92173, 92174, 92175, 92194, 92195, 92196, 92197, 92198, 92199, 96021, 96022, 96052, 96053, - 96057View allHide

    Fluorescent CF® dye tyramide conjugates for tyramide signal amplification (TSA). A horseradish peroxidase (HRP)-mediated method for high-density labeling of a target protein or nucleic acid in situ.

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    Fluorescent CF® dye tyramide conjugates for tyramide signal amplification (TSA). A horseradish peroxidase (HRP)-mediated method for high-density labeling of a target protein or nucleic acid in situ.

     

    Ready-to-Use Tyramide Amplification Buffer, 1X

    Sizes: 25 mL, 100 mLCatalog #: 22027-25mL, - 22027-100mLView allHide

    Ready-to-use buffer for tyramide signal amplification; simply add CF® dye or other tyramide probes to the buffer for signal amplification.

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    Ready-to-use buffer for tyramide signal amplification; simply add CF® dye or other tyramide probes to the buffer for signal amplification.

     

    Tyramide Amplification Buffer Plus

    From: $110 Sizes: 250 reactions, 1000 reactionsCatalog #: 22029, - 22029-TView allHide

    Tyramide signal amplification buffer with enhanced sensitivity. For use with CF® dye or other tyramide probes for signal amplification.

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    250 reactions
    1000 reactions
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    Tyramide signal amplification buffer with enhanced sensitivity. For use with CF® dye or other tyramide probes for signal amplification.

     

    Biotin-XX Tyramide

    From: $271 Sizes: 0.5 mgCatalog #: 92176

    This biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    0.5 mg
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    This biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    Cyanine 555 Tyramide

    From: $281 Sizes: 0.5 mgCatalog #: 96020

    This  Cyanine 555  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    0.5 mg
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    This  Cyanine 555  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    DNP Tyramide

    From: $268 Sizes: 0.5 mgCatalog #: 96019

    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    0.5 mg
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    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    Fluorescein Tyramide

    From: $268 Sizes: 0.5 mgCatalog #: 96018

    This fluorescein tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

    Size
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    Qty
    0.5 mg
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    This fluorescein tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.