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Tyramides and Tyramide Signal Amplification Kits

Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger signal compared to conventional immunofluorescence.

Biotium’s Tyramide Amplification Kits feature our bright and photostable CF® dyes or biotin with anti-mouse, anti-rabbit, or HRP streptavidin conjugates. The kits offer benefits of high sensitivity and signal-to-background ratio, enabling the visualization of low-abundance targets that cannot be efficiently labeled using traditional immunostaining methods. CF® dye, biotin, and other tyramides are also available separately.

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    Tyramide Amplification Kit with HRP Streptavidin and CF® Dye/Biotin Tyramide

    From: $450 Sizes: 1 kitCatalog #: 33002, 33005, 33008, 33011, 33014, 33017, - 33020View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

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    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye/Biotin Tyramide

    From: $450 Sizes: 1 kitCatalog #: 33001, 33004, 33007, 33010, 33013, 33016, - 33019View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 kit
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    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye/Biotin Tyramide

    From: $450 Sizes: 1 kitCatalog #: 33000, 33003, 33006, 33009, 33012, 33015, - 33018View allHide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 kit
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    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

     

    Cyanine 555 Tyramide

    From: $273 Sizes: 0.5 mgCatalog #: 96020

    This  Cyanine 555  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    This  Cyanine 555  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    DNP Tyramide

    From: $260 Sizes: 0.5 mgCatalog #: 96019

    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    Fluorescein Tyramide

    From: $260 Sizes: 0.5 mgCatalog #: 96018

    This fluorescein tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    This fluorescein tyramide conjugate can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    Biotin-XX Tyramide

    From: $263 Sizes: 0.5 mgCatalog #: 92176

    This biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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    This biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

     

    CF® Dye Tyramides

    From: $263 Sizes: 0.5 mgCatalog #: 92170, 92171, 92172, 92173, 92174, 92175, 92194, 92195, 92196, 92197, 92198, 92199, 96021, 96022, 96052, 96053, - 96057View allHide

    CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

    Conjugation
    Size
    Catalog #
    price
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    0.5 mg
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    CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.