Tyramides and Tyramide Signal Amplification Kits
Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger signal compared to conventional immunofluorescence.
Biotium’s Tyramide Amplification Kits feature our bright and photostable CF® dyes or biotin with anti-mouse, anti-rabbit, or HRP streptavidin conjugates. The kits offer benefits of high sensitivity and signal-to-background ratio, enabling the visualization of low-abundance targets that cannot be efficiently labeled using traditional immunostaining methods. CF® dye, biotin, and other tyramides are also available separately.