Super-resolution microscopy encompasses a broad family of imaging techniques that push beyond the diffraction limit of traditional light microscopy, revealing the finer details of biological structures. These techniques rely on extremely precise control over the excitation, emission, and image acquisition of fluorescently labeled cells and tissues. Consequently, the quality and efficiency of super-resolution imaging relies heavily on the fluorescent properties of the probe. Recent advances in super-resolution microscopy have demonstrated the importance of high-performance fluorescent probes. Biotium’s CF® dyes have met this demand with industry-leading brightness, photostability, and photochemical switching properties over other commercially available fluorescent probes. Moreover, CF® dyes have been published in dozens of studies for a wide range of super-resolution applications.

As Lehmann and colleagues report in their publication in the Journal of Biophotonics:

Antibody Labeling Kits

Our Mix-n-Stain™ Antibody Labeling Kits dramatically simplify the process of preparing fluorescently-labeled antibodies, offering bright and stable conjugates in only 30 minutes. The resulting conjugates offer comparable performance to antibodies labeled with other commercially available labeling kits.

• Label with your choice of 29 CF® dyes in only 30 minutes
• Stable covalent labeling with no purification required
• Compatible with common antibody stabilizers
• Labeling kits designed for Nanobodies® (ie. camelid single variable or VHH domains) also available

Reactive CF® Dyes

Developing a custom probe for super-resolution applications? Biotium offers CF® dyes in a wide array of reactive chemistries to suit your research needs.

• Succinimidyl esters for labeling amine-reactive NHS esters
• Maleimide and MTS CF® dyes for labeling thiols
• Aminooxy and hydrazide reactive CF® dyes for labeling carbonyl groups or carbohydrates
• Azides, alkynes, & BCN CF® dyes for highly specific bioorthogonal labeling

MemBrite® Fix stains are validated for super-resolution and other specialized imaging applications

MemBrite® Fix stains are novel reactive fluorescent dye stains that react irreversibly with cell surface proteins, for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, where surface staining varies between cell types, MemBrite® Fix dyes react widely with cell surface proteins for more uniform staining.

Several MemBrite® Fix dyes have been validated in super-resolution imaging applications or 2-photon microscopy. MemBrite® Fix-ST dyes are specialized dyes recommended for super-resolution imaging by STORM. MemBrite® Fix-ST dyes can also be used for standard microscopy applications; however, MemBrite® Fix dyes are generally more photostable than MemBrite® Fix-ST dyes. See the table on the right for a list of MemBrite® Fix dyes validated for super-resolution and other specialized imaging applications.

LysoView™ Dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (see highlighted citation below), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.