FIXABLE DEAD CELL STAINS WITH A WIDE SELECTION OF COLORS
Fixable and stable labeling of dead cells for flow cytometry or microscopy
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes enter dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. Live-or-Dye™ stains are offered in a wide selection of 14 colors for easy panel design. This includes spectrally unique dyes designed for taking advantage of enhanced multiplexing by spectral flow cytometry.
Live-or-Dye™ staining is extremely stable and is compatible with intracellular staining protocols without interference with immunostaining.
- Wide selection of 14 dye colors for easy panel design
- Exceptionally bright dyes for excellent live/dead separation
- Withstands fixation and permeabilization
- No loss of brightness after fixation
- For flow cytometry or fluorescence microscopy
- Dyes designed to fit gaps in spectral flow panels
- The most options for the 633 nm and 808 nm lasers
- Trial sizes and sampler kits available
Withstands fixation and permeabilization
Live-or-Dye™ Fixable Viability Staining Kits
Compatible lasers (nm)
Optimal detection channels
|32018, 32018-T||Live-or-Dye™ 330/410||355, 375||BUV395|
|32002, 32002-T||Live-or-Dye™ 350/448||355, 375||DAPI|
|32014,32014-T||Live-or-Dye™ 375/600||355, 375, 405||Spectral scan, BUV615, BV605||Developed for spectral cytometry|
|32003, 32003-T||Live-or-Dye™ 405/452||405||Pacific Blue®, BV421, BV450|
|32009, 32009-T||Live-or-Dye™ 405/545||405||AmCyan, BV510|
|32004, 32004-T||Live-or-Dye™ 488/515||488||FITC||Validated for microscopy|
|32012, 32012-T||Live-or-Dye™ 510/550||488, 532||Spectral scan||Developed for spectral cytometry|
|32005, 32005-T||Live-or-Dye™ 568/583||488, 532, 561||PE, PI||Validated for microscopy|
|32006, 32006-T||Live-or-Dye™ 594/614||488, 532, 561||PI, PE-CF®594, PE-Texas Red®||Validated for microscopy|
|32015, 32015-T||Live-or-Dye™ 615/740||633-640||Spectral scan, APC-Cy®7||Developed for spectral cytometry|
|32007, 32007-T||Live-or-Dye™ 640/662||633-640||APC||Validated for microscopy|
|32013, 32013-T||Live-or-Dye™ 665/685||633-640||Spectral scan, AF700||Developed for spectral cytometry|
|32008, 32008-T||Live-or-Dye™ 750/777||633-640, 785||APC-Cy®7||The brightest dye for the APC-Cy®7 channel|
|32011, 32011-T||Live-or-Dye™ 787/808||785, 808||APC-Cy®7, IR800|
Widest range of colors for easy panel design
Live/dead stains are useful probes to include in flow cytometry panels because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis. However, the limited number of dye colors from other commercially available live/dead stains can make choosing the right fluorophores difficult when designing highly complex panels. Live-or-Dye™ Fixable Viability Staining Kits are offered in a wide selection of live/dead dye colors, allowing maximal flexibility when designing multi-color flow panels (Fig. 3)
Spectrally Unique Dyes Designed for Spectral Flow Cytometry
Spectral flow cytometry is a rapidly growing technology with significantly enhanced multiplexing capabilities over conventional flow cytometry. Where conventional flow cytometer instruments can detect panels with more than a dozen fluorophores, spectral flow cytometers can accommodate panels with upwards of 40 dyes. Biotium offers spectrally unique Live-or-Dye™ stains designed specifically to take advantage of enhanced multiplexing by spectral flow ctyometry.
Live-or-Dye™ Stains for Spectral Flow
Learn more about spectral flow cytometry and our high-performance CF® dyes developed to fill emission gaps between available fluorophores.
New: Live-or-Dye™ Sampler Kits for Flow Cytometry
Live-or-Dye™ stains are available in two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Live-or-Dye™ Sampler Kits table below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.
Live-or-Dye™ Sampler Kits
Included dyes: Ex/Em (Cat No.)
|Live-or-Dye™ Fixable Viability Sampler Kit, Standard||32016||• 350/448 (32002A)|
• 405/545 (32009A)
• 488/515 (32004A)
• 568/583 (32005A)
• 640/662 (32007A)
|Designed for use on most standard flow cytometry laser and filter configurations|
|Live-or-Dye™ Fixable Viability Sampler Kit, Spectral||32017||• 350/448 (32002A)|
• 375/600 (32014A)
• 510/550 (32012A)
• 615/740 (32015A)
• 665/685 (32013A)
|Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores|
Bright and photostable dyes for microscopy
It is often very useful to identify dead cells for microscopic analysis as well, particularly when performing live-cell imaging experiments. In microscopy, live/dead stains allow unambiguous visual discrimination of dead cells. Live-or-Dye™ Fixable Viability Staining Kits are bright and photostable dyes that work just as well for microscopy as they do for flow cytometry, with negligible signal in live cells and strong signal in dead cells (Fig. 5).
Four stains have been validated for fluorescence microscopy. The other dyes are expected to work as well, as long as the microscope has the appropriate excitation and emission settings.
Live-or-Dye™ Stains validated for fluorescence microscopy
LIVE-OR-DYE NUCFIX™ RED
Live-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells.
Unlike other commonly used nuclear stains such as propidium iodide (PI) or DRAQ7™, Live-or-Dye NucFix™ labeling is covalent, so the dye doesn’t transfer between cells after fixation. Live-or-Dye NucFix™ Red can be used for flow cytometry or fluorescence microscopy (Fig. 6).
YEAST-SPECIFIC LIVE-OR-DYE™ STAINING KITS
Yeast specific kits include a Live-or-Dye™ stain with a yeast or fungal all-cell stain.
The Yeast Live-or-Dye™ Live/Dead Staining Kit combines a cell-permeant green dye, Thiazole Orange, which concentrates in the nucleus of live cells, with Live-or-Dye™ 568/583 (Fig. 7). Co-staining with the two dyes allows live/dead discrimination of yeast by fluorescence microscopy or flow cytometry.
The Yeast Viability Staining Kits take advantage of the unique fungal cell wall, by combining a Live-or-Dye™ dead cell stain with a fluorescent conjugate of the cell wall probe Concanavalin A (Con A) (Fig. 8). These kits are available in 3 color combinations for maximum experimental flexibility.
|Catalog No.||Kit Name||Live-or-Dye™ Color||Other Dye|
|31064||Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit||Live-or-Dye™ 568/583 (stains dead cells red)||Thiazole Orange (stains all cells green)|
|31063-1||Yeast Viability Staining Kit (Green/Red)||Live-or-Dye™ 594/614 (stains dead cells red)||CF®488A ConA (stains all cell walls green)|
|31063-2||Yeast Viability Staining Kit (Red/Blue)||Live-or-Dye™ 405/452 (stains dead cells blue)||CF®594 ConA (stains all cell walls red)|
|31063-3||Yeast Viability Staining Kit (Red/Far-Red)||Live-or-Dye™ 568/583 (stains dead cells red)||CF®640R ConA (stains all cell walls far-red)|
Draq7 is a trademark of Biostatus, Ltd. Texas Red and Pacific Blue are registered trademarks of Thermo Fisher Scientific. Cy dye is a registered trademark of GE Healthcare.