Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis. In microscopy, live/dead stains allow unambiguous visual discrimination of dead cells.
Covalent labeling of dead cells for flow or microscopy
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes enter dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells (Figure 1).
Live-or-Dye™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Biotium offers a selection of eight bright and photostable Live-or-Dye™ stains for maximal flexibility in multi-color analysis. We also offer Live-or-Dye NucFix™ Red, a unique fixable nuclear-specific dead cell stain.
Principle of Live-or-Dye™ discrimination of live and dead cells.
Live-or-Dye™ for Flow Cytometry
Choose from eight bright Live-Or-Dye™ stains for flow cytometry.
Live-or-Dye™ for Fluorescence Microscopy
Four bright and photostable Live-or-Dye™ stains are validated for microscopy.
Live-or-Dye NucFix™ Red
Live-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells.
Unlike other commonly used nuclear stains such as propidium iodide or DRAQ7™, Live-or-Dye NucFix™ labeling is covalent, so the dye doesn’t transfer between cells after fixation. Live-or-Dye NucFix™ Red can be used for flow cytometry or fluorescence microscopy.
Live-or-Dye™ Fixable Viability Staining Kits
|Catalog No.||Viability Dye||Laser line||Emission filter||Abs/Em Max||Applications|
|32002, 32002-T||Live-or-Dye 350/448||355 nm||DAPI or Violet||347/448 nm||Flow Cytometry|
|32003, 32003-T||Live-or-Dye 405/452||405 nm||Pacific Blue®||408/452 nm||Flow Cytometry|
|32009, 32009-T||Live-or-Dye 405/545||405 nm||AmCyan||395/545 nm||Flow Cytometry,
|32004, 32004-T||Live-or-Dye 488/515||488 nm||FITC||490/515 nm||Flow Cytometry,
|32005, 32005-T||Live-or-Dye 568/583||488 or 561 nm||PE||562/583 nm||Flow Cytometry,
|32006, 32006-T||Live-or-Dye 594/614||488 or 561 nm||PE-Texas Red®||593/614 nm||Flow Cytometry,
|32007, 32007-T||Live-or-Dye 640/662||633 or 640 nm||APC||642/662 nm||Flow Cytometry|
|32008, 32008-T||Live-or-Dye 750/777||633 or 640 nm||APC-Cy®7||755/777 nm||Flow Cytometry,
|32010, 32010-T||Live-or-Dye NucFix Red||488 or 532 nm||PE-Texas Red®||520/593 nm||Flow Cytometry,
Also see our other Products for Flow Cytometry.
Draq7 is a trademark of Biostatus, Ltd. Texas Red and Pacific Blue are registered trademarks of Thermo Fisher Scientific. Cy dye is a registered trademark of GE Healthcare.