Fluo-4 AM Ester

50018
string(39) "$190.00"
Price:
 $190.00
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Product Description

Membrane-permeant AM ester form of Fluo-4 that can be loaded into cells via incubation. Fluo-4 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to fluo-4 by endogenous esterases once the dye is inside the cells. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal.  An important application of Fluo-4 AM ester is its use in high throughput drug screening.

  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution
  • Ex/Em of Ca2+–bound form: 494/506 nm
  • Kd for Ca2+ in buffer: ~335 nM
  • C51H50F2N2O23
  • MW: 1096.95
  • [273221-67-3]
  1. Cell Calcium (2000), doi: 10.1054/ceca.1999.0095

Additional Information

Size

10 x 50 ug

MSDS (PDF): MSDS 50018

Protocol (PDF): psds-50018

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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life