Membrane-permeant AM ester form of Fluo-4 that can be loaded into cells via incubation. Fluo-4 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to fluo-4 by endogenous esterases once the dye is inside the cells. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. An important application of Fluo-4 AM ester is its use in high throughput drug screening.
- Orange red solid soluble in DMSO
- Store -20°C and protect from light, especially when in solution
- Ex/Em of Ca2+–bound form: 494/506 nm
- Kd for Ca2+ in buffer: ~335 nM
- MW: 1096.95