Fluo-4 AM Ester
Membrane-permeant form of Fluo-4 calcium indicator. Fluo-4 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fluo-4 by endogenous esterases once inside cells.
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Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Pluronic® F-127 20% Solution in DMSO
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.
Calcium Calibration Buffer Kit
This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.
Probenecid, Sodium Salt, Water Soluble
Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.
Fura-2, AM Ester
Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.