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Azide, Alkyne, BCN, Tetrazine & TCO

Bioorthogonal labeling involves reactions between functional groups that do not react with native targets in the cell. Therefore bioorthogonal conjugation can be highly specific and controlled. We offer dyes with a variety of functional groups for bioorthogonal labeling. The original bioorthogonal chemistries require copper catalysis, which can cause oxidative damage to cells and proteins. Newer chemistries require lower copper concentrations or are copper-free, avoiding this problem.

Azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. Picolyl azide undergoes the same reaction but at a much lower copper concentration. Azides also can undergo Staudinger ligation with phosphine containing compounds. Alkynes can be used as monomers to copolymerize into labeled polymers. BCN reacts with azide and methyltetrazine reacts with TCO (trans-cyclooctene) by copper-free reactions.

We offer a wide selection of our bright and photostable CF® dyes or biotin with your choice of bioorthogonal functional groups. Learn more about CF® Dyes.

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    CF® Dye Alkyne

    From: $279 Sizes: 0.5 mgCatalog #: 92086, 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide

    CF® dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

     

    CF® Dye Azide

    From: $279 Sizes: 0.5 mgCatalog #: 92080, 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide

    CF® dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

     

    CF® Dye BCN

    From: $330 Sizes: 0.5 mgCatalog #: 96070, 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide

    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

     

    CF® Dye Methyltetrazine

    From: $291 Sizes: 1 mgCatalog #: 96028, 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide

    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

     

    CF® Dye Picolyl Azide

    From: $289 Sizes: 0.5 mgCatalog #: 92187, 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

     

    CF® Dye TCO

    From: $291 Sizes: 1 mgCatalog #: 96040, 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide

    CF® dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.

     

    CF® Dye Tetrazine

    From: $294 Sizes: 1 mgCatalog #: 96056, 96055, - 96054View allHide

    CF® dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

     

    Biotin Methyltetrazine

    Sizes: 1 mgCatalog #: 96038

    Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

     

    Biotin TCO

    From: $291 Sizes: 1 mgCatalog #: 96050

    Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

     

    Cyanine Dye Alkyne

    From: $168 Sizes: 1 mgCatalog #: 92100, - 92101View allHide

    Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

    Dye Color
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

     

    Biotin Alkyne

    From: $70 Sizes: 1 mgCatalog #: 92168

    Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

     

    Biotin Azide

    From: $70 Sizes: 1 mgCatalog #: 92167

    Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

     

    Biotin BCN

    From: $330 Sizes: 1 mgCatalog #: 92169

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

     

    Biotin Picolyl Azide

    From: $281 Sizes: 0.5 mgCatalog #: 92186

    Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

     

    MTS-BCN

    From: $382 Sizes: 1 mgCatalog #: 96023

    MTS-BCN is a fast-reacting and highly selective thiol-reactive label for attaching BCN to thiol groups on biomolecules. BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    MTS-BCN is a fast-reacting and highly selective thiol-reactive label for attaching BCN to thiol groups on biomolecules. BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.