Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Azide, Alkyne, BCN, Tetrazine & TCO

Bioorthogonal labeling involves reactions between functional groups that do not react with native targets in the cell. Therefore bioorthogonal conjugation can be highly specific and controlled. We offer dyes with a variety of functional groups for bioorthogonal labeling. The original bioorthogonal chemistries require copper catalysis, which can cause oxidative damage to cells and proteins. Newer chemistries require lower copper concentrations or are copper-free, avoiding this problem.

Azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. Picolyl azide undergoes the same reaction but at a much lower copper concentration. Azides also can undergo Staudinger ligation with phosphine containing compounds. Alkynes can be used as monomers to copolymerize into labeled polymers. BCN reacts with azide and methyltetrazine reacts with TCO (trans-cyclooctene) by copper-free reactions.

We offer a wide selection of our bright and photostable CF® dyes or biotin with your choice of bioorthogonal functional groups. Learn more about CF® Dyes.

Showing all 15 results

    CF® Dye Tetrazine

    From: $285 Sizes: 1 mgCatalog #: 96056, 96055, - 96054View allHide

    CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

     

    CF® Dye TCO

    From: $275 Sizes: 1 mgCatalog #: 96040, 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide

    CF dye with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF dye with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

     

    Biotin TCO

    From: $275 Sizes: 1 mgCatalog #: 96050

    Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

     

    Biotin Methyltetrazine

    From: $275 Sizes: 1 mgCatalog #: 96038

    Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

     

    CF® Dye Methyltetrazine

    From: $275 Sizes: 1 mgCatalog #: 96028, 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide

    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

    Conjugation
    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

     

    MTS-BCN

    From: $360 Sizes: 1 mgCatalog #: 96023

    BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. MTS-BCN is a fast-reacting and highly selective thiol-reactive label that permits one to attach BCN to a thiol group.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. MTS-BCN is a fast-reacting and highly selective thiol-reactive label that permits one to attach BCN to a thiol group.

     

    Biotin Picolyl Azide

    From: $273 Sizes: 0.5 mgCatalog #: 92186

    Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

     

    Biotin Alkyne

    From: $68 Sizes: 1 mgCatalog #: 92168

    Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form biotin labeled polymers.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form biotin labeled polymers.

     

    Biotin Azide

    From: $68 Sizes: 1 mgCatalog #: 92167

    Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with phosphine containing compound.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with phosphine containing compound.

     

    Biotin BCN

    From: $320 Sizes: 1 mgCatalog #: 92169

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

     

    CF® Dye Picolyl Azide

    From: $273 Sizes: 0.5 mgCatalog #: 92187, 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

    Conjugation
    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

     

    Cyanine Dye Alkyne

    From: $158 Sizes: 1 mgCatalog #: 92100, - 92101View allHide

    Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

    Dye Color
    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

     

    CF® Dye BCN

    From: $310 Sizes: 0.5 mgCatalog #: 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide

    CF dye with BCN, an alternative to fluorescently labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

    Conjugation
    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF dye with BCN, an alternative to fluorescently labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

     

    CF® Dye Alkyne

    From: $263 Sizes: 0.5 mgCatalog #: 92086, 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide

    CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

    Conjugation
    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

     

    CF® Dye Azide

    From: $263 Sizes: 0.5 mgCatalog #: 92080, 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide

    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

    Conjugation
    Size
    Catalog #
    price
    Qty
    0.5 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.