Support & Resources


Environmental Sustainability

Earth-Friendly and Safer Products

GelRed®, GelGreen®, and PAGE GelRed® non-mutagenic alternatives to EtBr and SYBR® dyes
Rapid and non-hazardous One-Step Protein Gel Stains to replace Coomassie, SYPRO®, and Oriole™ dyes
High performance, non-mutagenic EvaGreen® dye and Forget-Me-Not™ master mixes
AccuBlue® and AccuGreen™ kits options with non-mutagenic dyes
See our full list of safer and Earth-friendly alternative products

Our Contribution to Sustainable Research

As a manufacturer and supplier of chemical reagents for research, we at Biotium understand the critical role we play in protecting the Earth’s natural resources. Consequently, our commitment to innovation not only entails pushing the boundaries of performance, but also how our products can contribute to a safer and more sustainable research environment.

For this reason, whenever possible we strive to develop products that can be disposed down the sink without harming wildlife. We also design our products with researchers’ safety in mind. And unlike other companies that claim their products are safe, we have data to back us up, including environmental safety reports from third party testing facilities. The dyes featured here are all classified as non-hazardous for waste disposal under California Title 22 regulations.

In addition, our primary manufacturing facility located in the San Francisco Bay Area is committed to minimizing its carbon footprint by implementing green methods to our systems and business practices. This includes maximizing biodegradable materials in our packaging and providing all product related documents electronically. Our facility also relies heavily on renewable energy by sourcing 80% of our power needs from solar energy.

Searching for Earth-Friendly Alternatives?

Look for this symbol

For convenience, we have labeled certain product pages and product search results for customers looking for more sustainable alternatives. Products labeled with this symbol will have at least one of the following features.

  • Non-toxic and less hazardous alternative
  • Environmentally friendly disposal
  • Greater efficiency for less waste of resources

Our facility is 80% powered by solar energy.

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DNA Gel Stains

GelRed® and GelGreen®

Safer alternatives to EtBr, SYBR® Safe, and others

GelRed® and GelGreen® are sensitive fluorescent DNA gel stains designed to replace highly toxic ethidium bromide (EtBr) and other so-called safe gel stains. Ames tests have confirmed that GelRed® and GelGreen® are non-mutagenic at concentrations well above the concentrations used for gel staining. Furthermore, environmental safety tests showed that GelRed® and GelGreen® are non-toxic to aquatic life, permitting disposal down the drain or in regular trash. To learn more, visit the GelRed® and GelGreen® technology page.

GelRed® and GelGreen® stains are also available pre-coated to ultra-pure LE agarose. Using GelRed® Agarose LE & GelGreen® Agarose LE is easy and convenient, and obviates the need to handle concentrated fluorescent dye.

PAGE GelRed® is a DNA gel stain for use in acrylamide gels. In designing PAGE GelRed® dye, we used a novel approach to make the dye membrane-impermeant without significantly increasing its size. Like the regular GelRed® and GelGreen®, it does not penetrate into cells, unlike other supposedly safe dyes.

See our safety data reports for our GelRed® and GelGreen® products below.

GelRed® & GelGreen® do not readily penetrate cells. HeLa cells were incubated for 30 minutes at 37°C with 1X SYBR® Safe, GelRed®, or GelGreen®. SYBR® Safe rapidly bound to DNA in live cells resulting in bright green nuclear staining. GelRed® and GelGreen® were unable to bind DNA in live cells, shown by the absence of fluorescence.

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Protein Gel Stains

One-Step protein gel stains are ready-to-use solutions for the staining of PAGE gels. They produce fast protein staining in a single step without fixation or washing. In addition to rapid results and simple staining, One-Step stains offer safer handling and disposal compared to Coomassie and other stains because they are entirely aqueous-based, without hazardous methanol or acetic acid.

Biotium offers several versions of One-Step stains for different visualization methods. One-Step Blue® can be detected by visible blue staining or by near-infrared fluorescence. One-Step Lumitein™ is a red fluorescent protein gel stain that is able to be detected using a UV light box, laser gel scanner or blue light illuminator like Gel-Bright™. One-Step Lumitein™ UV is optimized for maximum sensitivity on a UV transilluminator. See our safety data report for our One-Step products on the right. To learn more please visit our One-Step protein gel stains technology page.


One-Step Blue® is an alternative to tedious staining protocols using Coomassie blue dye, and can be imaged visually or using a near-infrared fluorescence imager.
Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or laser gel scanner
One-Step Lumitein™ UV Gel Stain is an alternative to Oriole™ and Flamingo™ Gel Stains. Best stain for UV transilluminator. It does not contain hazardous solvents or shrink gels.

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EvaGreen® qPCR Master Mixes

EvaGreen® Dye is the first and only PCR dye to date designed to be environmentally safe.

Handling and disposal of DNA-binding dyes in PCR master mixes can be a health and environmental issue due to their ability to cause mutations. Indeed, SYBR® Green I is found to be even more environmentally toxic than ethidium bromide, one of the best known mutagens. SYBR® Green I has been suggested to interfere with DNA repair mechanisms in cells, and as a result it potentiates genotoxicity of chemicals as well as DNA damage by UV light.

Although no safety data are available on other PCR and HRM dyes (e.g., SYTO9, LC Green, BRYT Green and ResoLight), those dyes are all known to enter cells in a matter of minutes, thus posing potential genotoxicity risk. With this in mind, Biotium’s scientists designed EvaGreen® Dye to be cell membrane-impermeant by increasing the molecular size and charge of the dye. Because EvaGreen® Dye is denied the chance to interact with genomic DNA in living cells, it is made much safer than the other dyes. Independent laboratory tests have confirmed that EvaGreen® is non-mutagenic, non-cytotoxic and safe to aquatic life. The dye has passed environmental hazardous waste regulation in the state of California (CCR title 22) for easy disposal down the drain. To learn more please visit our EvaGreen® Dye technology page.

Learn more about our Forget-Me-Not™ qPCR Master Mixes which are hot-start EvaGreen® dye-based master mixes for use in real time PCR applications and DNA melt curve analysis. They are available in no, low or high ROX formulations. There are also options available for two-color tracking, to reduce pipetting errors.

See our safety data report for EvaGreen® Dye below.

EvaGreen® Dye is safer than SYBR® Green I due to the fact that it is cell membrane-impermeant, and therefore cannot gain access to the DNA in the nuclei of living cells.

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DNA Quantitation Kits

Biotium offers a wide selection of fluorescence-based DNA quantitation kits that vary in concentration range, instrument compatibility, and detection channel. Our AccuBlue® High Sensitivity dsDNA Quantitation Kit for microplate readers uses non-toxic and non-mutagenic EvaGreen® Dye for safer handling and easy disposal. Similarly, our AccuGreen™ Broad Range dsDNA Quantitation Kit designed for Thermo’s Qubit® fluorometer also uses a non-toxic and non-mutagenic DNA-binding dye. Learn more about our complete line of kits on our DNA & RNA quantitation technology page.

AccuBlue® High Sensitivity dsDNA Quantitation Kit

  • Highly specific for dsDNA
  • Uses a non-toxic, non-mutagenic EvaGreen® Dye
  • Linear detection range from 0.2-100 ng dsDNA
  • Designed for fluorescence microplate readers
  • Green fluorescence emission

AccuGreen™ Broad Range dsDNA Quantitation Kit

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Uses a non-toxic, non-mutagenic dye
  • Linear detection range from 2-1000 ng dsDNA
  • Replaces Qubit® dsDNA BR Assay Kit for cost savings
  • Green fluorescence emission

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Complete List of Earth-Friendly Products

Product Name Catalog Number Application Safety Features
GelRed® 10,000X in water 41001-41003 DNA Gel Stains Safer, non-mutagenic, and non-toxic alternatives to EtBr

Designed to be cell membrane impermeable

Certified under CCR Title 22 as non-toxic. Suitable for non-hazardous disposal
GelGreen® 10,000X in water 41004-41005
GelRed® 3X in water 41001
6X GelRed® Prestain Loading Buffer, Blue Tracking Dye 41009-41010
GelRed® Prestain Plus 6X DNA Loading Dye 41011
GelRed® Agarose LE 41029
GelGreen® Agarose LE 41030
One-Step Blue®
21003 Protein Gel Stains 100% Water-based formulation

No need for acetic acid, methanol, microwaving, washing or fixing

Certified under CCR Title 22 as non-toxic

Suitable for drain disposal after a simple pH neutralization step
One-Step Lumitein™ 21004
One-Step Lumitein™ UV 21005
EvaGreen® Dye, 20X in Water 31000 DNA Binding Dye for qPCR Environmentally safe, non-mutagenic, and non-cytotoxic
EvaGreen® Dye, 2000X in DMSO 31019
EvaGreen® Plus Dye, 20X in Water 31077
Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking) 31041-31042 qPCR Master Mixes Includes environmentally safe, non-mutagenic, and non-cytotoxic EvaGreen® Dye
Forget-Me-Not™ EvaGreen® qPCR Master Mix (Premixed Low ROX or High ROX) 31045-31046
AccuBlue® High Sensitivity dsDNA Quantitation Kit with Standards 31006 DNA Quantitation Non-toxic and non-mutagenic DNA-binding dyes
AccuGreen™ Broad Range dsDNA Quantitation Kit 31069
AccuGreen™ Broad Range dsDNA Quantitation Solution 31070
EvaEZ™ Fluorometric Polymerase Activity Assay Kit 29051 Polymerase Activity Assay Fluorescence based assay kit for measuring activity of nucleic acid polymerase without using radioisotopes
CoverGrip™ Coverslip Sealant 23005 Coverslip sealant Coverslip sealant made with d-limonene, a natural, environmentally-friendly alternative to nail-polish

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