Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

CF®750, CF®770, CF®790, & CF®800

Unrivaled near-IR dyes

Exceptionally bright & photostable
Compatible with LI-COR® Odyssey® and in vivo imaging
CF®750 validated in STORM super-resolution imaging
CF® Dye Spectrum:
CF®350 Dye

CF®350 is a blue fluorescent dye

300400500600700800900Wavelength (nm)CF350
Technical Summary

Abs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: ~ 496
Excitation source: UV
Replaces: Alexa Fluor® 350, AMCA,
DyLight® 350

Features

Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive

View Technology
CF®405S Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405S
Technical Summary

Abs/Em Maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~ 1,169
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 405, Cascade Blue®,
DyLight® 405

Features

Brighter than Alexa Flour®  408

View Technology
CF®405M Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405M
Technical Summary

Abs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Excitation laser line: 405 nm
Replaces: Pacific Blue®, BD Horizon™ V450

Features

More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM

View Technology
CF®405L Dye

A 405 nm-excitable dye with orange fluorescence emission

300400500600700800900Wavelength (nm)CF405L
Technical Summary

Abs/Em Maxima: 395/545 nm
Extinction coefficient: 24,000
Molecular weight: ~1573
Excitation laser line: 405 nm
Replaces: Pacific Orange®

Features
View Technology
CF®430 & CF®440 Dyes

Photostable 405 nm-excitable dyes with green fluorescence

300400500600700800900Wavelength (nm)CF430CF440
Technical Summary

CF®430
Abs/Em Maxima: 426/498 nm
Extinction coefficient: 40,000
Molecular weight: ~429
Excitation laser line: 405 nm
Replaces: Pacific Green®, BD Horizon™ V500,
Krome Orange™
CF®440
Abs/Em Maxima: 440/515 nm
Extinction coefficient: 40,000
Molecular weight: ~716
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 430

Features

Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive

View Technology
CF®450 Dye

A green fluorescent dye with unique spectral properties

300400500600700800900Wavelength (nm)CF450
Technical Summary

Abs/Em Maxima: 450/538 nm
Extinction coefficient: 40,000
Molecular weight: ~689
Excitation laser line: 405 nm

Features
View Technology
CF®488A Dye

A superior green fluorescent dye

300400500600700800900Wavelength (nm)CF488A
Technical Summary

Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~914
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 488, DyLight® 488,
fluorescein (aka FITC, FAM), Cy®2

Features

Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive

View Technology
CF®514 Dye

Alternative green fluorescent dye

300400500600700800900Wavelength (nm)CF514
Technical Summary

Abs/Em Maxima: 516/548 nm
Extinction coefficient: 105,000
Molecular weight: ~1216
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 514

Features

Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing

View Technology
CF®532 Dye

A bright orange fluorescent dye for the 532 nm laser

300400500600700800900Wavelength (nm)CF532
Technical Summary

Abs/Em Maxima: 527/558 nm
Extinction coefficient: 96,000
Molecular weight: ~ 685
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532

Features

Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Highly water-soluble and pH-insensitive

View Technology
CF®535ST Dye

An orange fluorescent dye designed for STORM super-resolution imaging

300400500600700800900Wavelength (nm)CF535ST
Technical Summary

Abs/Em Maxima: 535/568 nm
Extinction coefficient: 95,000
Molecular weight: ~728
Excitation laser line: 532 nm

Features
View Technology
CF®543 Dye

An orange fluorescent dye ideal for the 543 nm laser

300400500600700800900Wavelength (nm)CF543
Technical Summary

Abs/Em Maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~ 870
Excitation laser line: 532 nm, 543 nm, or
546 nm
Direct replacement for: Alexa Fluor® 546,
TAMRA

Features

Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive

View Technology
CF®555 Dye

A bright and photostable orange-red dye

300400500600700800900Wavelength (nm)CF555
Technical Summary

Abs/Em Maxima: 555/565 nm
Extinction coefficient: 150,000
Molecular weight: ~ 901
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor®
555, ATTO 550, Cy®3, DyLight® 549,
Rhodamine

Features

Brighter than Cy®3
Highly water-soluble
Validated in multicolor STORM super-resolution imaging

View Technology
CF®568 Dye

Outshines Alexa Fluor®568

300400500600700800900Wavelength (nm)CF568
Technical Summary

Abs/Em Maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~ 714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red

Features

Yields much brighter antibody conjugates than Alexa
Fluor® 568
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging

View Technology
CF®570 & CF®583 Dyes

Bright red fluorescent dyes

300400500600700800900Wavelength (nm)CF570CF583
Technical Summary

CF®570 Technical Summary

Abs/Em maxima: 568/591 nm
Extinction coefficient: 150,000
Molecular weight: ~2998
Excitation laser line: 561 to 568 nm
Replaces: Alexa Fluor® 568, ATTO 565, DY-560, Rhodamine Red

CF®583 Technical Summary

Abs/Em maxima: 583/606 nm
Extinction coefficient: 150,000
Molecular weight: ~3127
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5

Features
View Technology
CF®594 Dye

Truly the brightest deep red dye

300400500600700800900Wavelength (nm)CF594
Technical Summary

Abs/Em Maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~730
Excitation laser line: 532 nm, 568 nm or
594 nm
Replaces: Alexa Fluor® 594, DyLight® 594,
Texas Red®

Features

Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy

View Technology
CF®594ST Dye

Deep red dye for STORM

300400500600700800900Wavelength (nm)CF594ST
Technical Summary

Abs/Em maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~868
Excitation laser line: 532 to 568 nm

Features
View Technology
CF®620R Dye

A bright and photostable far-red dye

300400500600700800900Wavelength (nm)CF620R
Technical Summary

Abs/Em Maxima: 617/639 nm
Extinction coefficient: 115,000
Molecular weight: ~ 738
Excitation laser line: 633 nm or 635 nm
Replaces: LightCycler® Red 640

Features

Highly water-soluble
Highly fluorescent and extremely photostable
Absorption/emission at 617/639 nm for use in FRET or multi-color detection

View Technology
CF®633 Dye

The best dye for 633/635 laser lines

300400500600700800900Wavelength (nm)CF633
Technical Summary

Abs/Em Maxima: 630/650 nm
Extinction coefficient: 100,000
Molecular weight: ~820
Excitation laser line: 633 nm or 635 nm
Replaces: Alexa Fluor® 633, Alexa Fluor®
647, Cy®5, DyLight® 633, DyLight® 649

Features

Yields the brightest antibody conjugates among spectrally similar dyes
Far more photostable than Alexa Fluor® 647
Compatible with TIRF, FIONA, and gSHRImP super-resolution imaging methods

View Technology
CF®640R Dye

A highly photostable far-red dye

300400500600700800900Wavelength (nm)CF640R
Technical Summary

Abs/Em Maxima: 642/662 nm
Extinction coefficient: 105,000
Molecular weight: ~ 832
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces Alexa Fluor® 647, ATTO 647N,
Cy®5, DyLight® 649

Features

Best photostability among Cy®5-like dyes
Yields highly fluorescent protein conjugates
Compatible with TIRF and FLIMP super-resolution microscopy

View Technology
CF®647 Dye

A highly fluorescent far-red dye

300400500600700800900Wavelength (nm)CF647
Technical Summary

Abs/Em Maxima: 650/665 nm
Extinction coefficient: 240,000
Molecular weight: ~ 1058
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces: Cy®5, Alexa Fluor® 647,
DyLight® 649

Features

Brighter than Cy®5
Highly water soluble and pH insensitive
Validated in multi-color super-resolution imaging by STORM

View Technology
CF®660C and CF®660R Dyes

Superior alternatives to Alexa Fluor® 660

300400500600700800900Wavelength (nm)CF660CCF660R
Technical Summary

CF®660C
Abs/Em Maxima: 667/685 nm
Extinction coefficient: 200,000
Molecular weight: ~ 3112
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APC

CF®660R
Abs/Em Maxima: 663/682 nm
Extinction coefficient: 100,000
Molecular weight: ~ 888
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APC

Features

CF®660C Features
Much brighter and more photostable than Alexa Fluor® 660
Compatible with multicolor super-resolution imaging by STORM

CF®660R Features
Brighter than Alexa Fluor® 660
Unrivaled photostability among spectrally similar dyes

View Technology
CF®680 and CF®680R Dyes

Two outstanding 680 nm-excitable dyes

300400500600700800900Wavelength (nm)CF680CF680R
Technical Summary

CF®680
Abs/Em Maxima: 681/698 nm
Extinction coefficient: 210,000
Molecular weight: ~ 3241
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680

CF®680R
Abs/Em Maxima: 680/701 nm
Extinction coefficient: 140,000
Molecular weight: ~ 912
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680

Features

CF®680 Features
The brightest among spectrally similar dyes
Validated in multicolor STORM and 3D super-resolution
microscopy
Compatible with LI-COR® Odyssey®

CF®680R Features
Unrivaled photostability among spectrally similar dyes
Compatible with STED and single molecule spectroscopy super-resolution imaging (see pp. 20-21)
Molecular weight compatible with nucleic acid labeling
Compatible with LI-COR® Odyssey®

View Technology
Near-Infrared CF® Dyes

Unrivaled near-IR dyes

Technical Summary

CF®750
Abs/Em Maxima: 755/777 nm
Extinction coefficient: 250,000
Molecular weight: ~ 3009
Excitation laser line: 633, 635, 680 or 685 nm
Replaces: Alexa Fluor® 750, Cy®7, DyLight® 750

CF®770
Abs/Em Maxima: 770/797 nm
Extinction coefficient: 220,000
Molecular weight: ~ 3138
Excitation laser line: 785 nm
Replaces: DyLight™ 800, IRDye 800CW

CF®790
Abs/Em Maxima: 784/806 nm
Extinction coefficient: 210,000
Molecular weight: ~ 3267
Excitation laser line: 785 nm
Replaces: Alexa Fluor® 790

CF®800
Abs/Em Maxima: 797/816
Extinction coefficient: 210,000
Molecular weight: ~3334
Excitation laser line: 785 nm
Spectrally similar to: Indocyanine Green

Features

Exceptionally bright and stable
Ideal for in vivo imaging
Compatible with LI-COR® Odyssey®
Superior signal-to-noise for bioconjugates
Validated in STORM microscopy

View Technology
CF® Dyes for Multi-Color Super-Resolution Microscopy

Synthetic Dyes for Super-Resolution Imaging

Technical Summary
Features

CF Dyes validated in SIM, STORM, STED, TIRF, and more
Superior brightness, photostability, and photochemical switching
Single label antibody conjugates for STORM
Growing list of publications

View Technology

Near-infrared (near-IR) dyes offer important advantages over traditional visible light dyes. Because cellular or tissue components produce minimal autofluorescence in the near-IR region, near-IR dyes have the potential to offer highly specific and sensitive detection in complex biological systems. Light with wavelength in the near-IR region has strong tissue penetration, allowing the use of near-IR dyes for in vivo fluorescence imaging. For Western blotting, near-IR dyes offer advantages over chemiluminescence in sensitivity, linearity, and multi-color detection.

Near-IR CF® dyes are next-generation long wavelength dyes representing a true breakthrough in the field. The current commercial near-IR dyes suffer from problems of limited fluorescence brightness due to excessive dye aggregation and poor stability. As a result of novel molecular engineering by scientists at Biotium, near-IR CF® dyes overcome these problems, resulting in exceptionally bright and photostable dyes. In addition, near-IR CF® dyes possess a proprietary structural feature that renders the dyes more water-soluble than other near-infrared dyes without introducing excessive charge. Consequently, a higher number of near-IR CF® dyes can be conjugated to a protein for maximal fluorescence, while still preserving conjugate specificity and long in vivo half-life. We offer four Near-IR CF® dyes. Also see our far-red/near-IR CF®680 & CF®680R dyes, which are also compatible with NIR western and the LI-COR® Odyssey®.

Figure 1. Normalized absorption and emission spectra of near-IR CF® dye goat anti-mouse conjugates in PBS. Click image to enlarge.

Near-Infrared CF® Dyes

Advantages

  • Highly water soluble
  • Exceptionally bright and stable
  • Superior signal-to-noise for conjugates
  • Long in vivo half-life

Applications


Technical Summary

CF®750

  • Abs/Em: 755/777 nm
  • ε: 250,000
  • Molecular weight: ~3009
  • Laser line: 633 to 685 nm
  • Replaces: Alexa Fluor® 750, Cy®7, DyLight® 750

CF®770

  • Abs/Em: 770/797 nm
  • ε: 220,000
  • Molecular weight: ~3138
  • Laser line: 785 nm
  • Replaces: DyLight® 800, IRDye® 800CW

CF®790

  • Abs/Em: 784/806 nm
  • ε: 210,000
  • Molecular weight: ~3267
  • Laser line: 785 nm
  • Replaces:
    Alexa Fluor® 790

CF®800

  • Abs/Em: 797/816 nm
  • ε: 210,000
  • Molecular weight: ~3334
  • Laser line: 785 nm
  • Spectrally similar to: Indocyanine Green


Figure 2. CF® has improved water solubility compared to spectrally similar dyes. Absorbance spectra of the indicated dyes show large shoulder peaks for Cy®7 and Alexa Fluor® 750 compared to CF®790. Shoulder peaks reflect non-fluorescent dye aggregates of dyes with poor water solubility.
Figure 3. CF®750 produces much brighter conjugates than spectrally similar dyes. Relative brightness of goat anti-mouse IgG conjugates of the indicated dyes at varying degrees of labeling (DOL, or number of dyes per antibody).

Figure 4. CF®750 is more photostable than spectrally similar dyes. Absorbance spectra of NIR dyes before (0 min, solid lines) and after exposure to sunlight for 30 minutes (30 min, dashed lines). After light exposure, CF®750 shows more absorbance than Cy®7 or Alexa Fluor® 750, showing less photobleaching of the dye.
Figure 5. CF®750 for in vivo small animal imaging. Tumors in mice were imaged using an IVIS® imaging system (Perkin Elmer) 24 hours (left), 48 hours (center), and 96 hours (right) after IV injection of CF®750 Avastin® conjugate. Image courtesy of Caliper Life Sciences.

Figure 6. CF®770 is brighter than spectrally similar dyes. Jurkat cells were stained with mouse anti-CD3 antibody or isotype control and goat anti-mouse conjugates of the dyes shown at varying degrees of labeling (DOL, or dye molecules per antibody). Fluorescence was analyzed on BD FACSCalibur flow cytometer in the FL4 channel. Bars represent geometric mean fluorescence.
Figure 7. CF® dye conjugates are brighter than IRDye® conjugates for NIR western. Western blotting of HeLa cell lysate (2 ug to 0.125 ug, lanes 1-5) for tubulin and COX IV, detected by IR®680LT or CF®680 (red) and IRDye® 800CW or CF®770 (green) secondary antibodies. Quantitation of bands showed approximately 50% brighter signal with CF® dyes compared to IRDye®. M: molecular weight marker.
Figure 8. CF®790 has fewer negative charges than Alexa Fluor® 790, resulting in more specific antibody conjugates. Western blots of HeLa lysate (two dilutions, lanes 1 and 2) were probed with mouse anti-tubulin antibody followed by goat anti-mouse conjugates of the indicated dyes. The Alexa Fluor® 790 conjugate shows multiple non-specific bands compared to CF®790. M: molecular weight marker.

CF is a registered trademark of Biotium, Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; IRDye and Odyssey are registered trademarks of LI-COR Biosciences.

CF®680 & CF®680R Dyes < Previous           CF® Dyes Overview

 

CF® Dye Succinimidyl Ester

From: $147 Sizes: 0.25 umol, 1 umolCatalog #: 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide