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CF® DYES

Next Generation Fluorescent Dyes

High-performance CF® dyes
Superior brightness, photostability, and solubility
Multi-color flexibility
28 CF® dyes across the visible, far-red & near-IR spectra
For NIR western or small animal imaging
The best performance for STORM, SIM, and other methods
Excellent labeling efficiency
More stable succinimidyl esters
Wide selection of products
Antibodies, conjugates, reactive dyes, and labeling kits
CF® Dye Spectrum:
CF®350 Dye

CF®350 is a blue fluorescent dye

300400500600700800900Wavelength (nm)CF350
Technical Summary

Abs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: ~ 496
Excitation source: UV
Replaces: Alexa Fluor® 350, AMCA,
DyLight® 350

Features

Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive

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CF®405S Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405S
Technical Summary

Abs/Em Maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~ 1,169
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 405, Cascade Blue®,
DyLight® 405

Features

Brighter than Alexa Flour®  408

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CF®405M Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405M
Technical Summary

Abs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Excitation laser line: 405 nm
Replaces: Pacific Blue®, BD Horizon™ V450

Features

More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM

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CF®405L Dye

A 405 nm-excitable dye with orange fluorescence emission

300400500600700800900Wavelength (nm)CF405L
Technical Summary

Abs/Em Maxima: 395/545 nm
Extinction coefficient: 24,000
Molecular weight: ~1573
Excitation laser line: 405 nm
Replaces: Pacific Orange®

Features
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CF®430 & CF®440 Dyes

Photostable 405 nm-excitable dyes with green fluorescence

300400500600700800900Wavelength (nm)CF430CF440
Technical Summary

CF®430
Abs/Em Maxima: 426/498 nm
Extinction coefficient: 40,000
Molecular weight: ~429
Excitation laser line: 405 nm
Replaces: Pacific Green®, BD Horizon™ V500,
Krome Orange™
CF®440
Abs/Em Maxima: 440/515 nm
Extinction coefficient: 40,000
Molecular weight: ~716
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 430

Features

Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive

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CF®450 Dye

A green fluorescent dye with unique spectral properties

300400500600700800900Wavelength (nm)CF450
Technical Summary

Abs/Em Maxima: 450/538 nm
Extinction coefficient: 40,000
Molecular weight: ~689
Excitation laser line: 405 nm

Features
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CF®488A Dye

A superior green fluorescent dye

300400500600700800900Wavelength (nm)CF488A
Technical Summary

Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~914
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 488, DyLight® 488,
fluorescein (aka FITC, FAM), Cy®2

Features

Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive

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CF®514 Dye

Alternative green fluorescent dye

300400500600700800900Wavelength (nm)CF514
Technical Summary

Abs/Em Maxima: 516/548 nm
Extinction coefficient: 105,000
Molecular weight: ~1216
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 514

Features

Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing

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CF®532 Dye

A bright orange fluorescent dye for the 532 nm laser

300400500600700800900Wavelength (nm)CF532
Technical Summary

Abs/Em Maxima: 527/558 nm
Extinction coefficient: 96,000
Molecular weight: ~ 685
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532

Features

Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Highly water-soluble and pH-insensitive

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CF®535ST Dye

An orange fluorescent dye designed for STORM super-resolution imaging

300400500600700800900Wavelength (nm)CF535ST
Technical Summary

Abs/Em Maxima: 535/568 nm
Extinction coefficient: 95,000
Molecular weight: ~728
Excitation laser line: 532 nm

Features
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CF®543 Dye

An orange fluorescent dye ideal for the 543 nm laser

300400500600700800900Wavelength (nm)CF543
Technical Summary

Abs/Em Maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~ 870
Excitation laser line: 532 nm, 543 nm, or
546 nm
Direct replacement for: Alexa Fluor® 546,
TAMRA

Features

Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive

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CF®555 Dye

A bright and photostable orange-red dye

300400500600700800900Wavelength (nm)CF555
Technical Summary

Abs/Em Maxima: 555/565 nm
Extinction coefficient: 150,000
Molecular weight: ~ 901
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor®
555, ATTO 550, Cy®3, DyLight® 549,
Rhodamine

Features

Brighter than Cy®3
Highly water-soluble
Validated in multicolor STORM super-resolution imaging

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CF®568 Dye

Outshines Alexa Fluor®568

300400500600700800900Wavelength (nm)CF568
Technical Summary

Abs/Em Maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~ 714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red

Features

Yields much brighter antibody conjugates than Alexa
Fluor® 568
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging

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CF®570 & CF®583 Dyes

Bright red fluorescent dyes

300400500600700800900Wavelength (nm)CF570CF583
Technical Summary

CF®570 Technical Summary

Abs/Em maxima: 568/591 nm
Extinction coefficient: 150,000
Molecular weight: ~2998
Excitation laser line: 561 to 568 nm
Replaces: Alexa Fluor® 568, ATTO 565, DY-560, Rhodamine Red

CF®583 Technical Summary

Abs/Em maxima: 583/606 nm
Extinction coefficient: 150,000
Molecular weight: ~3127
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5

Features
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CF®594 Dye

Truly the brightest deep red dye

300400500600700800900Wavelength (nm)CF594
Technical Summary

Abs/Em Maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~730
Excitation laser line: 532 nm, 568 nm or
594 nm
Replaces: Alexa Fluor® 594, DyLight® 594,
Texas Red®

Features

Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy

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CF®594ST Dye

Deep red dye for STORM

300400500600700800900Wavelength (nm)CF594ST
Technical Summary

Abs/Em maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~868
Excitation laser line: 532 to 568 nm

Features
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CF®620R Dye

A bright and photostable far-red dye

300400500600700800900Wavelength (nm)CF620R
Technical Summary

Abs/Em Maxima: 617/639 nm
Extinction coefficient: 115,000
Molecular weight: ~ 738
Excitation laser line: 633 nm or 635 nm
Replaces: LightCycler® Red 640

Features

Highly water-soluble
Highly fluorescent and extremely photostable
Absorption/emission at 617/639 nm for use in FRET or multi-color detection

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CF®633 Dye

The best dye for 633/635 laser lines

300400500600700800900Wavelength (nm)CF633
Technical Summary

Abs/Em Maxima: 630/650 nm
Extinction coefficient: 100,000
Molecular weight: ~820
Excitation laser line: 633 nm or 635 nm
Replaces: Alexa Fluor® 633, Alexa Fluor®
647, Cy®5, DyLight® 633, DyLight® 649

Features

Yields the brightest antibody conjugates among spectrally similar dyes
Far more photostable than Alexa Fluor® 647
Compatible with TIRF, FIONA, and gSHRImP super-resolution imaging methods

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CF®640R Dye

A highly photostable far-red dye

300400500600700800900Wavelength (nm)CF640R
Technical Summary

Abs/Em Maxima: 642/662 nm
Extinction coefficient: 105,000
Molecular weight: ~ 832
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces Alexa Fluor® 647, ATTO 647N,
Cy®5, DyLight® 649

Features

Best photostability among Cy®5-like dyes
Yields highly fluorescent protein conjugates
Compatible with TIRF and FLIMP super-resolution microscopy

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CF®647 Dye

A highly fluorescent far-red dye

300400500600700800900Wavelength (nm)CF647
Technical Summary

Abs/Em Maxima: 650/665 nm
Extinction coefficient: 240,000
Molecular weight: ~ 1058
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces: Cy®5, Alexa Fluor® 647,
DyLight® 649

Features

Brighter than Cy®5
Highly water soluble and pH insensitive
Validated in multi-color super-resolution imaging by STORM

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CF®660C and CF®660R Dyes

Superior alternatives to Alexa Fluor® 660

300400500600700800900Wavelength (nm)CF660CCF660R
Technical Summary

CF®660C
Abs/Em Maxima: 667/685 nm
Extinction coefficient: 200,000
Molecular weight: ~ 3112
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APC

CF®660R
Abs/Em Maxima: 663/682 nm
Extinction coefficient: 100,000
Molecular weight: ~ 888
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APC

Features

CF®660C Features
Much brighter and more photostable than Alexa Fluor® 660
Compatible with multicolor super-resolution imaging by STORM

CF®660R Features
Brighter than Alexa Fluor® 660
Unrivaled photostability among spectrally similar dyes

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CF®680 and CF®680R Dyes

Two outstanding 680 nm-excitable dyes

300400500600700800900Wavelength (nm)