Protease Substrates
See below for fluorescent substrates for proteases such as caspases, peptidases, elastases, granzyme B, cathepsins, and others.
Caspase substrates include the novel, non-toxic, fluorogenic NucView® caspase-3 substrates, available in 3 colors.
TEV Protease Kit
29090-1000U, - 29090-10000UView allHide
TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).
Ac-DEVD-R110
A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.
NucView® Caspase-3 Enzyme Substrates
10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide
Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.
NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit
Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.
Z-AA-R110-PEG
Z-AA-R110-PEG (Rhodamine 110-PEG, N-CBZ-L-alanyl-L-alanine amide) is a substrate for elastate.
BZiPAR
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).
Z-FR-R110
Z-FR-R110 (Rhodamine 110, bis-(N-CBZ-L-phenylalanyl-L-arginine amide), dihydrochloride) is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.
Ac-DEVD-AMC
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
BZAR
BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
Z-FR-AMC
Z-FR-AMC is a substrate for serine proteases, including cathepsins, kallikrein and plasmin.
Rhodamine 110 (high purity)
Rhodamine 110 can be used as a reference standard for rhodamine 110-based peptidase substrates.
NucView® 488 & MitoView™ 633 Apoptosis Assay Kit
No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.
NucView® 488 Caspase-3 Assay Kit for Live Cells
30029, - 30029-TView allHide
Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-1, 30009-2, - 30009-3View allHide
A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).
Z-AAD-R110
Z-AAD-R110 is a substrate for granzyme B. Granzyme B from T lymphocytes and natural killer cells induces apoptosis in target cells by activating caspases and causing mitochondrial cytochrome C release.
R110-PEG (Rhodamine 110-PEG)
R110-PEG can be used as a reference standard for peptidase substrates based on the dye.