Protease Substrates
See below for fluorescent substrates for proteases such as caspases, peptidases, elastases, granzyme B, cathepsins, and others.
Caspase substrates include the novel, non-toxic, fluorogenic NucView® caspase-3 substrates, available in 3 colors.
Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V
These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.
These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.
NucView® 488 & MitoView™ 633 Apoptosis Assay Kit
No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.
No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.
NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit
Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.
Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.
NucView® 488 Caspase-3 Assay Kit for Live Cells
Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.
Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.
NucView® Caspase-3 Enzyme Substrates
Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.
Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.
A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).
A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit
A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.
A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).
A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).
Ac-DEVD-R110
A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.
A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.
Ac-DEVD-AMC
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
Ac-IETD-R110
Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
BZAR
BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
BZiPAR
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).
D2R
D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
R110-PEG (Rhodamine 110-PEG)
R110-PEG can be used as a reference standard for peptidase substrates based on the dye.
R110-PEG can be used as a reference standard for peptidase substrates based on the dye.