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Protease Substrates

See below for fluorescent substrates for proteases such as caspases, peptidases, elastases, granzyme B, cathepsins, and others.

Caspase substrates include the novel, non-toxic, fluorogenic NucView® caspase-3 substrates, available in 3 colors.

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    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $386 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

     
    Product

    NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

    From: $386 Sizes: 100 assaysCatalog #: 30062

    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

     
    Product

    NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit

    From: $338 Sizes: 100 assaysCatalog #: 30072

    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

     
    Product

    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $103 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (25 assays)
    100 assays
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    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

     
    Product

    NucView® Caspase-3 Enzyme Substrates

    From: $71 Sizes: 10 uL (1 mM in DMSO), 100 uL (1 mM in DMSO), 10 uL (1 mM in PBS), 100 uL (1 mM in PBS)Catalog #: 10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide

    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

    Dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
    Catalog #
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    Qty
    10 uL (1 mM in DMSO)
    100 uL (1 mM in DMSO)
    10 uL (1 mM in PBS)
    100 uL (1 mM in PBS)
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    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

     
    Product

    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $156 Sizes: 25 assays, 100 assaysCatalog #: 30008-1, - 30008-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 assays
    100 assays
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    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

     
    Product

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

    From: $51 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30009-1, 30009-2, - 30009-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 assays
    100 assays
    1,000 assays
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    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

     
    Product

    Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit

    From: $156 Sizes: 25 assays, 100 assaysCatalog #: 30011-1, - 30011-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 assays
    100 assays
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    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

     
    Product

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

    From: $51 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30012-1, 30012-2, - 30012-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 assays
    100 assays
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    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

     
    Product

    Ac-DEVD-R110

    From: $95 Sizes: 1 mgCatalog #: 10226

    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mg
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    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

     
    Product

    Ac-DEVD-AMC

    From: $236 Sizes: 5 mgCatalog #: 10202

    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 mg
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    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

     
    Product

    Ac-IETD-R110

    From: $293 Sizes: 5 mgCatalog #: 10207

    Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 mg
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    Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.

     
    Product

    BZAR

    From: $113 Sizes: 5 mgCatalog #: 10205

    BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 mg
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    BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.

     
    Product

    BZiPAR

    From: $187 Sizes: 5 mgCatalog #: 10208

    BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 mg
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    BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).

     
    Product

    D2R

    From: $123 Sizes: 2 mgCatalog #: 10210

    D2R

    D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    2 mg
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    D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.

     
    Product

    R110-PEG (Rhodamine 110-PEG)

    From: $198 Sizes: 5 mgCatalog #: 10225

    R110-PEG can be used as a reference standard for peptidase substrates based on the dye.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 mg
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    R110-PEG can be used as a reference standard for peptidase substrates based on the dye.