Protease Substrates
See below for fluorescent substrates for proteases such as caspases, peptidases, elastases, granzyme B, cathepsins, and others.
Caspase substrates include the novel, non-toxic, fluorogenic NucView® caspase-3 substrates, available in 3 colors.
Showing 1–16 of 25 results
Ac-DEVD-AMC
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
Ac-IETD-R110
Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
BZAR
BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
BZiPAR
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).
BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.
Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit
Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.
Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.
D2R
D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V
These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.
These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.
NucView® 405 Caspase-3 Substrate, 1 mM in PBS
NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.
NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.
NucView® 488 and MitoView™ 633 Apoptosis Assay Kit
This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.
This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.
NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit
NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.
NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.
NucView® 488 Caspase-3 Assay Kit for Live Cells
NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in DMSO
NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.
NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.