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PCR & DNA AMPLIFICATION

Bright, sensitive and environmentally safe; ideal for qPCR and many other applications
2-color or 1-color tracking allows you to spot pipetting errors at a glance
Suitable for all fluorescent probe-based PCR methods including TaqMan® or Molecular Beacons
Chemnically modified hot-start taq; no activity at RT and activates in only 2 minutes
Easy, accurate, and non-radioactive nucleic acid polymerase activity assay
dNTPs, ROX reference dye, hot-start/warm-start enzyme modification kits, and more

EVAGREEN®: A NEXT-GENERATION QPCR DYE

EvaGreen® dye is a next-generation DNA-binding dye with features ideal for use in quantitative real-time PCR (qPCR) and many other applications. Biotium scientists designed the dye by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, stability and fluorescence spectra of the dye. The result of our efforts is a dye superior to SYBR® Green I and other commercial PCR or high-resolution melt curve (HRM) dyes.*

EvaGreen® dye is used in all of Biotium’s dye-based qPCR master mixes, which combine superior brightness and sensitivity, with the ability to do sensitive melt curve analysis in the same reaction. See EvaGreen® Dye Technology to learn more.

EvaGreen® dye is non-toxic, non-mutagenic, and not hazardous to aquatic life.

* Practicing HRM may require a license from Idaho Technologies, Inc.

Figure 1. EvaGreen® dye binds to dsDNA via a "release-on-demand" mechanism.
Figure 1. EvaGreen® dye binds to dsDNA via a “release-on-demand” mechanism.
iconsevagreen
Figure 2. EvaGreen® is non-toxic, non mutagenic and non hazardous to aquatic life.
Product NameCatalog NumberSize
EvaGreen® Dye, 20X in water (trial size)31000-T1 mL
EvaGreen® Dye, 20X in water 310005 x 1 mL
EvaGreen® Dye, 2000X in DMSO3101950 uL

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FORGET-ME-NOT™ EVAGREEN® QPCR MASTER MIX

Forget-Me-Not™ qPCR Master Mix is a hot-start EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template buffer containing a higher concentration of blue dye. When you add the 2X Forget-Me-Not™ Master Mix to your reaction, it appears light blue. Then, when you add template containing Forget-Me-Not™  Template Buffer to the reaction, the color turns dark blue (Figure 1, right). Forget-Me-Not™ allows you to see at a glance whether you forgot to add master mix or template to any of your reactions, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples.

To learn more about our master mixes, please see Forget-Me-Not™ Master Mix Technology.

evagreen vs. sybr green
Figure 3. A comparison of the raw fluorescence signal from qPCR reactions performed with two EvaGreen® master mixes (Forget-Me-Not™ EvaGreen® and Fast EvaGreen®) and QuantiNova SYBR® Green. EvaGreen® dye is less inhibitory than SYBR® green, allowing for a much brighter signal.

FORGET-ME-NOT™ UNIVERAL PROBE QPCR MASTER MIX

Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons). Forget-Me-Not™ Universal Probe Master Mix shows excellent concordance of results in singleplex and multiplex reactions, has broad instrument compatibility, and can be used in both standard and fast protocols. A separate kit is also available with ROX, if your instrument requires ROX reference dye.

To learn more about our master mixes, please see Forget-Me-Not™ Master Mix Technology.

forget me not universal probe master mix
Figure 4. Forget-Me-Not Universal Probe qPCR Master Mix works exceptionally well for mutiplexed probe PCR reactions.
Product NameCatalog NumberFeatures
Forget-Me-Not™ qPCR Master Mix31041EvaGreen dye-based master mix with a unique two-color system for tracking which reagents you’ve added to your reaction.
Forget-Me-Not™ qPCR Master Mix with ROX31042EvaGreen dye-based master mix with a unique two-color system for tracking which reagents you’ve added to your reaction, with ROX reference dye.
Forget-Me-Not™ Universal Probe qPCR Master Mix31043High-performance master mix for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
Forget-Me-Not™ Universal Probe qPCR Master Mix with ROX31044High-performance master mix for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. With ROX reference dye.

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CHEETAH™ HOTSTART TAQ

Cheetah™ Taq is a chemically-modified hot-start DNA polymerase. Using a proprietary method developed at Biotium, Cheetah™ Taq is modified to have absolutely no activity at room temperature, but be completely activated after two minutes at 95°C.

FEATURES

  • Fast activation:
    Takes less than 2 minutes to activate, much faster than AmpliTaq Gold and HotStarTaq®.
  • Excellent activity recovery:
    Better recoveries of both 5′-3′-polymerase and 5′-exonuclease activities following activation than AmpliTaq Gold.
  • Improved stability:
    Better stability than AmpliTaq Gold during storage.
  • pH compatibility:
    Can be activated at alkaline pH (8.5-9) for optimal Taq activity and more specific target amplification.
hot start enzymes
Figure 5. Cheetah™ Taq recovers full activity faster than other hot-start enzymes such as AmpliTaq Gold.
Product NameCatalog NumberSize
Cheetah™ HotStart Taq DNA Polymerase29050500 U

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EVA-EZ™ POLYMERASE ACTIVITY ASSAY

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen® dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase. The assay was developed for the measurement of Taq DNA polymerase activity. It can also be used for other DNA polymerases such as Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I. The activity assay can be conducted at temperatures from 4°C to 75°C.

evaez assays taq dna polymerase
Figure 6. Sample data from an EvaEZ assays, showing a titration of Taq DNA Polymerase.
Product NameCatalog NumberSize
EvaEZ™ Fluorometric Polymerase Activity Assay Kit29051200 assays

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VIABILITY PCR DYES & KITS

Biotium is proud to have developed the cutting-edge technology of viability PCR, a technique in which cell viability can be assessed by treating a sample with a DNA-binding dye, followed by a qPCR or isothermal amplification reaction. To learn more about this technology and our portfolio of reagents for viability PCR, please see Viability PCR Technology.

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PCR ACCESSORY REAGENTS & RELATED PRODUCTS

Biotium offers a wide range of PCR/qPCR accessory products to assist you in your DNA amplification experiments, including:

  • dNTPs, sold as mixes or in separate tubes
  • Fluorescently-labeled and biotinylated nucleotides (dUTP, dCTP & others)
  • ROX Reference Dye
  • PCR dye (see EvaGreen®)
  • Taq DNA Polymerase (see Cheetah™ Taq)
  • HotStart and WarmStart™ Polymerase Modification Kits
Product NameCatalog NumberSize
dNTP Mix, 10 mM Each400545 x 1 mL
dNTP Mix, 25 mM Each400531 mL
dNTP Set, 100 mM Each40052250 uL Each
5-Aminoallyl dUTP (AA-dUTP), 10 mM Solution40020100 uL
BrdU40024100 mg
dCTP CF® Dye Conjugates9 dye color options5 or 25 nmol
dUTP CF® Dye Conjugates8 dye color options5 or 25 nmol
ROX Reference Dye290525 x 1 mL
EvaGreen® Dye, 2000X in DMSO3101950 uL
EvaGreen® Dye, 20X in water310005 x 1 mL
Cheetah™ Hot-Start Taq Polymerase29050500 U
HotStart Polymerase Modification Kit290541 kit modifies 0.5 mg of polymerase
WarmStart™ Enzyme Modification Kit290531 kit modifies 0.5 mg of protein

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Forget-Me-Not EvaGreen qPCR Master Mix (2-Color Tracking)

From: $58 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide

Forget-Me-Not Universal Probe qPCR Master Mix

From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)Catalog #: 31043-1, 31043-T, 31044-1, - 31044-TView allHide

EvaGreen Dye, 20X in water

From: $44 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31000, - 31000-TView allHide

Cheetah HotStart Taq DNA Polymerase

From: $221 Sizes: 500 UCatalog #: 29050

EvaEZ Fluorometric Polymerase Activity Assay Kit

From: $368 Sizes: 2 x 1 mLCatalog #: 29051

HotStart Polymerase Modification Kit

From: $194 Sizes: Trial size, 1 kitCatalog #: 29054, - 29054-TView allHide

WarmStart Enzyme Modification Kit

From: $194 Sizes: Trial size, 1 kitCatalog #: 29053, - 29053-TView allHide

dNTP Mix, 10 mM each

From: $368 Sizes: 5 x 1 mLCatalog #: 40054