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GelRed Nucleic Acid Gel Stain

GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step.

GelRed Nucleic Acid Gel Stain
GelRed Nucleic Acid Gel Stain
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Catalog #

41001, 41002, 41003, 41002-1, 41003-1, - 41003-TView allHide

Size

Trial size (0.1 mL), 0.5 mL, 10 mL, 4 L

Format

10,000X in water, 3X in water, 10,000X in DMSO

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Product Description

GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step. GelRed™ and EB have virtually the same spectra, so you can directly replace EB with GelRed™ without changing your existing imaging system.

For detailed information, please visit the DNA Stain Technology page.

GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed™ can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed™ is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning. For detailed protocols for use, please download the GelRed™ Product Information Sheet. Also see our GelRed™ and GelGreen™ Frequently Asked Questions (FAQs).

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed™/GelGreen™ Safety Report.

GelRed™ 3X in water (catalog no. 41001) is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L cubitainer. Biotium also offers GelRed™ 10,000X in water (catalog no. 41003) and GelRed™ 10,000X in DMSO (catalog no. 41002). GelRed™ in water is a newer, safer formulation and our recommended format. We continue to offer GelRed™ in DMSO for established users who do not wish to alter their protocols.

Also see GelGreen™ Nucleic Acid Gel Stain, a safer replacement for SYBR® gel stains, which is compatible with visible light excitation.

FEATURES

  • Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
  • Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash
  • Ultra-sensitive: Much more sensitive than EtBr and SYBR® Safe
  • Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
  • Simple to use: Very simple procedures for precast or post-electrophoresis gel staining
  • Compatible with a standard UV transilluminator : Replaces EtBr with no optical setting change
  • Compatible with downstream applications : Compatible with gel purification, restriction digest, sequencing and cloning

Download the GelRed & GelGreen Flyer for more information.

Please also see our EvaGreen® dye, a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR (qPCR). By incorporating a smart “release-on-demand” DNA-binding technology, EvaGreen™ has low PCR inhibition while exhibiting superior sensitivity. Similar to GelRed™, EvaGreen™ is non-toxic, non-mutagenic, and remarkably stable.

GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.

References

1. Eur Biophys J (2014), doi: 10.1007/s00249-014-0995-4

Download a list of selected References for GelRed and GelGreen.

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Support & faq

GelRed™ and GelGreen™ Nucleic Acid Gel Stains

The main difference between GelRed™ and GelGreen™ is their fluorescence excitation and emission wavelengths. GelRed™ has red fluorescence, similar to ethidium bromide. GelGreen™ has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen™ is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed™ and GelGreen™ have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed™ is more sensitive for staining single stranded nucleic acids than GelGreen™. GelRed™ is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen™ for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed™ Product Line

View the GelGreen™ Product Line

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

GelRed™ is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen™ has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen™ is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.