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Gel-Bright™ Laser Diode Gel Illuminator

A laser diode-based gel illuminator for visualizing fluorescent nucleic acid and protein gels. Avoids UV light hazards and offers exceptional performance for widely used green and red dyes.

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Product Description

The Gel-Bright™ Laser Diode Gel Illuminator features a novel laser diode (LD)-based illumination technology that offers comparable performance to UV transilluminators while avoiding the hazards of UV light which can damage skin and eyes. UV light can also induce cross-linking and nicking of DNA in samples. Unlike blue LED gel illuminators such as Dark Reader or Safe Imager™ that are not compatible with red dyes, the Gel-Bright™ Laser Diode Gel Illuminator works well with both green dyes and widely used red dyes like GelRed®, ethidium bromide (EtBr), and One-Step Lumitein™ Protein Gel Stain.

The Laser Diode Advantage

  • Novel LD illumination: Superior performance for imaging fluorescent gels
  • Brighter signal: More sensitive than UV for green dyes
  • Versatile: Works well with both green and red dyes, unlike blue LED illuminators
  • Safer: Eliminates UV light hazards for user and DNA samples

Other Features

  • Optimized lighting angle for even illumination
  • Adjustable light intensity
  • Multi-hinged adjustable amber filter for optimal signal
  • Easy access to the gel for gel slice excision
  • Compact and portable design
  • Gel-Bright™ Imaging Hood included

Superior Performance Over Blue LED Iluminators

Gel illuminators have traditionally relied on UV illumination for visualization of fluorescently labeled nucleic acids and proteins. However, UV-based imaging can damage skin and eyes, as well as your DNA samples. LED-based illuminators were developed as a safer alternative to UV, but often have high background and dimmer signal due to excessive ambient light and poor excitation efficiency.

In partnership with Biotium, OMEC Medical has developed a new type of gel illuminator that uses LDs optimized for brighter and clearer fluorescent imaging of gels. The new laser diode-based Gel-Bright™ offers better sensitivity over UV-based transilluminators when imaging green dyes such as GelGreen® or SYBR® Green, as well as significantly better performance over blue LED gel illuminators for red dyes such as GelRed®, ethidium bromide (EtBr), and One-Step Lumitein™ Protein Gel Stain.

Convenient Imaging Hood Included

Capture an image of your gel with any device using the included Gel-Bright™ Imaging Hood. Simply place the imaging hood over the filter and image the gel through the small opening at the top using a camera or smart phone.

Note: Purchase of the Gel-Bright™ Laser Diode Gel Illuminator (E90005) includes the Gel-Bright™ Imaging Hood (E90005B). However, the Gel-Bright™ Imaging Hood (E90005B) may also be purchased as a standalone accessory.

Free Samples of Pre-Coated GelRed® & GelGreen® Agarose Included

Each purchase of a Gel-Bright™ Laser Diode Gel Illuminator will include a free sample of GelRed® Agarose LE (Cat. No. 41029-5G) and GelGreen® Agarose LE (Cat. No. 41030-5G). These are safer and more convenient pre-coated agarose formats that remove the extra step of adding dye to your agarose.

Safe Imager™ vs. Gel-BrightLaser Diode Gel Illuminator

Comparison of Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator and Gel-Bright™ Laser Diode Gel Illuminator. An overview (top) is shown with the Safe Imager™ 2.0 Blue-Light Transilluminator (left) and Gel-Bright™ Laser Diode Gel Illuminator (right) in the same field of view, both with GelRed® (left) and GelGreen® (right) stained gels. The gels are a two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) that were separated on 1% agarose TBE gels. From left to right, loading amounts were 25, 50, 100, or 200 ng/lane. The cyan (Safe Imager™) and green (Gel-Bright™) boxes indicate the field of view for the zoomed images of the gels shown below. Click to enlarge.

Old Gel-Bright™ vs. Gel-BrightLaser Diode Gel Illuminator

Comparison of first generation Gel-Bright™ LED Gel Illuminator and new Gel-Bright™ Laser Diode Gel Illuminator. An overview (top) is shown with the Gel-Bright™ LED Gel Illuminator (left) and Gel-Bright™ Laser Diode Gel Illuminator (right) in the same field of view, both with GelGreen® (left) and GelRed® (right) stained gels. The gels are a two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) that were separated on 1% agarose TBE gels. From left to right, loading amounts were 200, 100, 50, or 25 ng/lane. The cyan (Old Gel-Bright™ LED) and green (New Gel-Bright™ Laser Diode) boxes indicate the field of view for the zoomed images of the gels shown below. Click to enlarge.

Sensitive Gel Imaging Without UV Hazards

Two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) were separated on 1% agarose TBE gels pre-cast with GelRed® or GelGreen®. Total DNA loaded per lane is indicated below the gels. The same gels were imaged using a UVP GelDoc-iT® (UV), Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator (Safe Imager™), first generation Gel-Bright™ LED Gel Illuminator (Blue LED), or new Gel-Bright™ Laser Diode Gel Illuminator (Gel-Bright™). On GelDoc-iT®, an EtBr filter was used to image GelRed® and a SYBR® filter was used for GelGreen®. For imaging on Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator, Gel-Bright™ LED Gel Illuminator, and Gel-Bright™ Laser Diode Gel Illuminator, the filters supplied with each device were used to photograph both GelRed® and GelGreen® stained gels. The Gel-Bright™ Laser Diode Gel Illuminator demonstrated the brightest signal for gels stained with GelGreen® and performed comparably to the UVP GelDoc-iT® for gels stained with GelRed®.

Compatible Dyes:

Learn more about our sensitive and less hazardous GelRed® and GelGreen® Nucleic Acid Gel Stains as well as our One-Step protein gel stains.

GelDoc-iT is a registered trademark of UVP/Analytik Jena GmbH; SYBR and SYPRO are registered trademarks of Thermo Fisher Scientific; Safe Imager is a trademark of Thermo Fisher Scientific.
 

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