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Gel-Bright™ Laser Diode Gel Illuminator

A laser diode-based gel illuminator for visualizing fluorescent nucleic acid and protein gels. Avoids UV light hazards and offers exceptional performance for widely used green and red dyes.

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Product Description

The Gel-Bright™ Laser Diode Gel Illuminator features a novel laser diode (LD)-based illumination technology that offers comparable performance to UV transilluminators while avoiding the hazards of UV light which can damage skin and eyes. UV light can also induce cross-linking and nicking of DNA in samples. Unlike blue LED gel illuminators such as Dark Reader or Safe Imager™ that are not compatible with red dyes, the Gel-Bright™ Laser Diode Gel Illuminator works well with both green dyes and widely used red dyes like GelRed®, ethidium bromide (EtBr), and One-Step Lumitein™ Protein Gel Stain.

The Laser Diode Advantage

  • Novel LD illumination: Superior performance for imaging fluorescent gels
  • Brighter signal: More sensitive than UV for green dyes
  • Versatile: Works well with both green and red dyes, unlike blue LED illuminators
  • Safer: Eliminates UV light hazards for user and DNA samples

Other Features

  • Optimized lighting angle for even illumination
  • Adjustable light intensity
  • Multi-hinged adjustable amber filter for optimal signal
  • Easy access to the gel for gel slice excision
  • Compact and portable design

Gel illuminators have traditionally relied on UV illumination for visualization of fluorescently labeled nucleic acids and proteins. However, UV-based imaging can damage skin and eyes, as well as your DNA samples. LED-based illuminators were developed as a safer alternative to UV, but often have high background and dimmer signal due to excessive ambient light and poor excitation efficiency.

Superior Performance Over Blue LED Iluminators

In partnership with Biotium, OMEC Medical has developed a new type of gel illuminator that uses LDs optimized for brighter and clearer fluorescent imaging of gels. The new laser diode-based Gel-Bright™ offers better sensitivity over UV-based transilluminators when imaging green dyes such as GelGreen® or SYBR® Green, as well as significantly better performance over blue LED gel illuminators for red dyes such as GelRed®, ethidium bromide (EtBr), and One-Step Lumitein™ Protein Gel Stain.

Free Samples of Pre-Coated GelRed® & GelGreen® Agarose Included

Each purchase of a Gel-Bright™ Laser Diode Gel Illuminator will include a free sample of GelRed® Agarose LE (Cat. No. 41029-5G) and GelGreen® Agarose LE (Cat. No. 41030-5G). These are safer and more convenient pre-coated agarose formats that remove the extra step of adding dye to your agarose.

Safe Imager™ vs. Gel-BrightLaser Diode Gel Illuminator

Comparison of Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator and Gel-Bright™ Laser Diode Gel Illuminator. An overview (top) is shown with the Safe Imager™ 2.0 Blue-Light Transilluminator (left) and Gel-Bright™ Laser Diode Gel Illuminator (right) in the same field of view, both with GelRed® (left) and GelGreen® (right) stained gels. The gels are a two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) that were separated on 1% agarose TBE gels. From left to right, loading amounts were 25, 50, 100, or 200 ng/lane. The cyan (Safe Imager™) and green (Gel-Bright™) boxes indicate the field of view for the zoomed images of the gels shown below. Click to enlarge.

Old Gel-Bright™ vs. Gel-BrightLaser Diode Gel Illuminator

Comparison of first generation Gel-Bright™ LED Gel Illuminator and new Gel-Bright™ Laser Diode Gel Illuminator. An overview (top) is shown with the Gel-Bright™ LED Gel Illuminator (left) and Gel-Bright™ Laser Diode Gel Illuminator (right) in the same field of view, both with GelGreen® (left) and GelRed® (right) stained gels. The gels are a two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) that were separated on 1% agarose TBE gels. From left to right, loading amounts were 200, 100, 50, or 25 ng/lane. The cyan (Old Gel-Bright™ LED) and green (New Gel-Bright™ Laser Diode) boxes indicate the field of view for the zoomed images of the gels shown below. Click to enlarge.

Sensitive Gel Imaging Without UV Hazards

Two-fold dilution series of Biotium’s Ready-to-Use 1 kb DNA Ladder (Cat. No. 31022) were separated on 1% agarose TBE gels pre-cast with GelRed® or GelGreen®. Total DNA loaded per lane is indicated below the gels. The same gels were imaged using a UVP GelDoc-iT® (UV), Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator (Safe Imager™), first generation Gel-Bright™ LED Gel Illuminator (Blue LED), or new Gel-Bright™ Laser Diode Gel Illuminator (Gel-Bright™). On GelDoc-iT®, an EtBr filter was used to image GelRed® and a SYBR® filter was used for GelGreen®. For imaging on Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator, Gel-Bright™ LED Gel Illuminator, and Gel-Bright™ Laser Diode Gel Illuminator, the filters supplied with each device were used to photograph both GelRed® and GelGreen® stained gels. The Gel-Bright™ Laser Diode Gel Illuminator demonstrated the brightest signal for gels stained with GelGreen® and performed comparably to the UVP GelDoc-iT® for gels stained with GelRed®.

Compatible Dyes:

Learn more about our sensitive and less hazardous GelRed® and GelGreen® Nucleic Acid Gel Stains as well as our One-Step protein gel stains.

GelDoc-iT is a registered trademark of UVP/Analytik Jena GmbH; SYBR and SYPRO are registered trademarks of Thermo Fisher Scientific; Safe Imager is a trademark of Thermo Fisher Scientific.
 

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