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Luciferase Substrates

For Firefly Luciferase, we offer D-Luciferin in a number of formats, including sodium and potassium salts, free acid, and caged.

For Renilla Luciferase, we offer many different Coelenterazine varients, including water-soluble Aquaphile™ Coelenterazine.

Showing 1–16 of 25 results

    Firefly & Renilla Luciferase Single Tube Assay Kit

    From: $95 Sizes: 50 assays, 150 assays, 1,000 assaysCatalog #: 30081-1, 30081-2, - 30081-TView allHide

    This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening.

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    This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening.

     

    Firefly Luciferase Assay Kit (Lyophilized)

    From: $68 Sizes: 150 assays, 1,000 assaysCatalog #: 30075-1, - 30075-2View allHide

    Firefly luciferase is widely used as a reporter gene for studying gene regulation and function, and for pharmaceutical screening. The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion.

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    Firefly luciferase is widely used as a reporter gene for studying gene regulation and function, and for pharmaceutical screening. The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion.

     

    Firefly Luciferase Assay Kit 2.0

    From: $53 Sizes: 50 assays, 150 assays, 1,000 assaysCatalog #: 30085-T, 30085-1, - 30085-2View allHide

    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

     

    Renilla Luciferase Assay Kit 2.0

    From: $58 Sizes: 50 assays, 150 assays, 1,000 assaysCatalog #: 30082-1, 30082-2, - 30082-TView allHide

    This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo.

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    This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo.

     

    Steady-Luc™ Firefly HTS Assay Kit

    From: $40 Sizes: 40 assays, 120 assays, 1,000 assays, 10,000 assaysCatalog #: 30028-1, 30028-2, 30028-3, - 30028-TView allHide

    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Biotium’s Steady-Luc™ HTS assay system is a homogeneous firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.

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    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Biotium’s Steady-Luc™ HTS assay system is a homogeneous firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.

     

    Steady-Luc™ Firefly HTS Assay Kit (Lyophilized)

    From: $81 Sizes: 120 assays, 1,000 assays, 10,000 assaysCatalog #: 30028-L1, 30028-L2, - 30028-L3View allHide

    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Biotium’s Steady-Luc™ HTS assay system is a homogeneous firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. The assay buffer is provided in lyophilized format for convenient room temperature shipping and -20°C storage.

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    Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Biotium’s Steady-Luc™ HTS assay system is a homogeneous firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. The assay buffer is provided in lyophilized format for convenient room temperature shipping and -20°C storage.

     

    D-Luciferin, Free Acid

    From: $56 Sizes: 10 mg, 50 mg, 1 gCatalog #: 10100, 10100-1, - 10100-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    D-Luciferin, Potassium Salt

    From: $56 Sizes: 10 mg, 50 mg, 1 gCatalog #: 10101, 10101-1, - 10101-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    D-Luciferin, Sodium Salt

    From: $66 Sizes: 10 mg, 50 mg, 1 gCatalog #: 10102, 10102-1, - 10102-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    Aquaphile™ Coelenterazine h, Lyophilized Solid

    From: $105 Sizes: 50 ug, 5 x 100 ugCatalog #: 10127, - 10127-50ugView allHide

    Aquaphile coelenterazine h is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

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    Aquaphile coelenterazine h is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

     

    Aquaphile™ Coelenterazine Native, Lyophilized Solid

    From: $105 Sizes: 50 ug, 5 x 100 ugCatalog #: 10126, - 10126-50ugView allHide

    Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

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    Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

     

    Coelenterazine (native)

    From: $56 Sizes: 50 ug, 250 ug, 1 mgCatalog #: 10110-1, 10110-2, - 10110View allHide

    Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase.

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    Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase.

     

    Coelenterazine 400a (DeepBlueC)

    From: $58 Sizes: 50 ug, 250 ug, 1 mgCatalog #: 10125-1, 10125-2, - 10125View allHide

    Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm.

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    Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm.

     

    Coelenterazine cp

    From: $56 Sizes: 50 ug, 250 ug, 1 mgCatalog #: 10112-1, 10112-2, - 10112View allHide

    Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.

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    Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.

     

    Coelenterazine e

    From: $105 Sizes: 50 ug, 250 ugCatalog #: 10124, - 10124-1View allHide

    Coelenterazine e is a synthetic derivative of coelenterazine, and is a superior substrate for Renilla luciferase.  Coelenterazine e generates nearly 40% more light than native coelenterazine does while the initial light intensity of the former is more than 7 times higher than that of the latter

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    Coelenterazine e is a synthetic derivative of coelenterazine, and is a superior substrate for Renilla luciferase.  Coelenterazine e generates nearly 40% more light than native coelenterazine does while the initial light intensity of the former is more than 7 times higher than that of the latter

     

    Coelenterazine f

    From: $56 Sizes: 50 ug, 250 ug, 1 mgCatalog #: 10114-1, 10114-2, - 10114View allHide

    Coelenterazine f is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is almost 20 times higher than that of the aequorin formed from native coelenterazine while its emission maximum is about 8 nm longer than that of the latter.

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    Coelenterazine f is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is almost 20 times higher than that of the aequorin formed from native coelenterazine while its emission maximum is about 8 nm longer than that of the latter.