Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
Biotium also offers Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) (catalog no. 30028L), a new packaging format that includes lyophilized assay buffer for convenient room temperature shipping and storage at -20°C.
- Steady-Luc Firefly Assay Buffer
- Simple: single step homogeneous assay.
- Glow kinetics: luminescence half life of about 3 hours.
- Economic: save up to 45% compared to other brands.
More Luciferase Kits
|30085||Firefly Luciferase Assay Kit 2.0||Flash-type firefly luciferase assay kit.|
|30075||Firefly Luciferase Assay Kit (Lyophilized)||Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.|
|99923||5X Firefly Lysis Buffer||Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.|
|30028||Steady-Luc Firefly HTS Assay Kit||Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.|
|30028L||Steady-Luc Firefly HTS Assay Kit (Lyophilized)||Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.|
|30082||Renilla Luciferase Assay Kit 2.0||Flash-type Renilla luciferase assay kit.|
|30081||Firefly and Renilla Luciferase Single Tube Assay Kit||Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.|
|99912||5X Passive Lysis Buffer||Extra lysis buffer for use with Renilla and Dual Luciferase Kits, only required if using well sizes larger than 24-well.|
|30020||ATP-Glo Bioluminometric Cell Viability Assay Kit||Flash-type luminescence-based assay for measuring cell viability or ATP concentration.|
|22003||Mini Cell Scrapers||Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.|