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Amine-Reactive Dyes

Succinimidyl esters (SE, also known as NHS esters) react with primary amines such as lysine residues in proteins. SE dyes are commonly used for labeling proteins and antibodies. The reaction requires basic pH for target amines to be reactive, and is usually performed in pH 8.3 sodium bicarbonate buffer.

We offer more than 25 colors of our bright and photostable CF® Dye SE and easy-to-use CF® Dye Labeling Kits, as well as a wide variety of other amine-reactive dyes and biotinylation reagents below. Biotium’s CF® dye SE are more resistant to hydrolysis than other SE, resulting in excellent reaction efficiency.

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    CF® Dye & Biotin SE Protein Labeling Kits

    From: $383 Sizes: 3 labelingsCatalog #: 92208, 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide

    This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.

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    This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.

     

    CF® Dye Succinimidyl Ester

    From: $147 Sizes: 0.25 umol, 1 umolCatalog #: 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide

    CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

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    CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

     

    VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging

    From: $415 Sizes: 3 labelingsCatalog #: 92160, 92161, 92162, - 92163View allHide

    This labeling kit comprises our superior near-IR amine-reactive CF® dye and other components necessary for carrying out antibody labeling and purification. It’s available with the following CF® dyes: CF®680, CF®750, CF®770, and CF®790.

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    This labeling kit comprises our superior near-IR amine-reactive CF® dye and other components necessary for carrying out antibody labeling and purification. It’s available with the following CF® dyes: CF®680, CF®750, CF®770, and CF®790.

     

    Cyanine Dye Succinimidyl Ester

    From: $158 Sizes: 1 mg, 10 mgCatalog #: 90025-10mg, 90025-1mg, 90048-10mg, 90048-1mg, 90049-10mg, 90049-1mg, 90016-10mg, - 90016-1mgView allHide

    Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters.

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    Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters.

     

    MB Acid

    From: $250 Sizes: 5 mgCatalog #: 40076

    MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

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    MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

     

    AMCA-X, SE

    From: $139 Sizes: 10 mgCatalog #: 90077

    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

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    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

     

    Carboxy-2′,7′- dichlorofluorescein diacetate, succinimidyl ester, 5 and 6 isomers

    From: $139 Sizes: 25 mgCatalog #: 90040

    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

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    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

     

    Carboxy-X-rhodamine free acid, 6-isomer

    From: $116 Sizes: 10 mgCatalog #: 90013

    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

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    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

     

    Carboxy-X-rhodamine succinimidyl ester, 5 and 6 isomers

    From: $139 Sizes: 25 mgCatalog #: 90005

    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

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    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

     

    Carboxy-X-rhodamine succinimidyl ester, 5 isomer

    From: $162 Sizes: 5 mgCatalog #: 90036

    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

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    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

     

    Carboxy-X-rhodamine succinimidyl ester, 6-isomer

    From: $162 Sizes: 5 mgCatalog #: 90037

    6-ROX, SE is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

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    6-ROX, SE is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

     

    Carboxyrhodamine 110 succinimidyl ester, 5 and 6 isomers

    From: $210 Sizes: 5 mgCatalog #: 90001

    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

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    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

     

    Carboxyrhodamine 110 succinimidyl ester, 5-isomer

    From: $436 Sizes: 5 mgCatalog #: 90030

    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

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    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

     

    Carboxyrhodamine 110 succinimidyl ester, 6-isomer

    From: $436 Sizes: 5 mgCatalog #: 90031

    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

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    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

     

    Carboxyrhodamine 110-PEO12, SE, 5 isomer

    From: $127 Sizes: 1 mgCatalog #: 90086

    5-CR110-PEO12 SE (full name: 5-Carboxyrhodamine 110-PEO12-propionate, succinimidyl ester, single isomer) has a long, water-soluble PEG spacer between the green fluorescent rhodamine dye and the amine-reactive SE group.

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    5-CR110-PEO12 SE (full name: 5-Carboxyrhodamine 110-PEO12-propionate, succinimidyl ester, single isomer) has a long, water-soluble PEG spacer between the green fluorescent rhodamine dye and the amine-reactive SE group.

     

    Carboxyrhodamine 110-PEO4, SE, 5 isomer

    From: $386 Sizes: 5 mgCatalog #: 90081

    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

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    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.