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Amine-Reactive Dyes

Succinimidyl esters (SE, also known as NHS esters) react with primary amines such as lysine residues in proteins. SE dyes are commonly used for labeling proteins and antibodies. The reaction requires basic pH for target amines to be reactive, and is usually performed in pH 8.3 sodium bicarbonate buffer.

We offer more than 25 colors of our bright and photostable CF® Dye SE and easy-to-use CF® Dye Labeling Kits, as well as a wide variety of other amine-reactive dyes and biotinylation reagents below. Biotium’s CF® Dye SE are more resistant to hydrolysis than other SE, resulting in excellent reaction efficiency.

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    Product

    CF® Dye & Biotin SE Protein Labeling Kits

    From: $433 Sizes: 3 labelingsCatalog #:
    92208
    , 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide

    Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

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    Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

     
    Product

    CF® Dye SE/TFP Ester

    From: $166 Sizes: 0.25 umol, 1 umolCatalog #:
    96095
    , 96094, 96084, 96073, 96078, 96068, 96067, 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 96092, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide

    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

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    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

     
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    VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging

    From: $468 Sizes: 3 labelingsCatalog #:
    92160
    , 92161, 92162, - 92163View allHide

    Kits for labeling & purification of near-IR antibody conjugates for small animal in vivo imaging, featuring Biotium’s industry-leading near-IR CF® Dyes.

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    Kits for labeling & purification of near-IR antibody conjugates for small animal in vivo imaging, featuring Biotium’s industry-leading near-IR CF® Dyes.

     
    Product

    Cyanine Dye Succinimidyl Ester

    From: $179 Sizes: 1 mg, 10 mgCatalog #:
    90025-10mg
    , 90025-1mg, 90048-10mg, 90048-1mg, 90049-10mg, 90049-1mg, 90016-10mg, - 90016-1mgView allHide

    Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555, Cyanine 647, Cyanine 680, and Cyanine 750 as high quality succinimidyl esters.

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    Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555, Cyanine 647, Cyanine 680, and Cyanine 750 as high quality succinimidyl esters.

     
    Product

    MB Acid

    From: $275 Sizes: 5 mgCatalog #: 40076

    MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

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    MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

     
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    AMCA-X, SE

    From: $161 Sizes: 10 mgCatalog #: 90077

    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

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    10 mg
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    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

     
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    Carboxy-2′,7′- dichlorofluorescein diacetate, succinimidyl ester, 5 and 6 isomers

    From: $161 Sizes: 25 mgCatalog #: 90040

    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

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    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

     
    Product

    Carboxy-X-rhodamine free acid, 6-isomer

    From: $135 Sizes: 10 mgCatalog #: 90013

    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

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    10 mg
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    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

     
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    Carboxy-X-rhodamine succinimidyl ester, 5 and 6 isomers

    From: $161 Sizes: 25 mgCatalog #: 90005

    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

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    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

     
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    Carboxy-X-rhodamine succinimidyl ester, 5 isomer

    From: $189 Sizes: 5 mgCatalog #: 90036

    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

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    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

     
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    Carboxy-X-rhodamine succinimidyl ester, 6-isomer

    From: $189 Sizes: 5 mgCatalog #: 90037

    6-ROX, SE (6-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

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    6-ROX, SE (6-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

     
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    Carboxyrhodamine 110 succinimidyl ester, 5 and 6 isomers

    From: $244 Sizes: 5 mgCatalog #: 90001

    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

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    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

     
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    Carboxyrhodamine 110 succinimidyl ester, 5-isomer

    From: $509 Sizes: 5 mgCatalog #: 90030

    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa Fluor® 488 because of its exceptional photostability and fluorescence insensitivity to pH.

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    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa Fluor® 488 because of its exceptional photostability and fluorescence insensitivity to pH.

     
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    Carboxyrhodamine 110 succinimidyl ester, 6-isomer

    From: $509 Sizes: 5 mgCatalog #: 90031

    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

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    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

     
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    Carboxyrhodamine 110-PEO4, SE, 5 isomer

    From: $451 Sizes: 5 mgCatalog #: 90081

    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

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    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

     
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    Carboxyrhodamine 110-X succinimidyl ester, 5 and 6 isomers

    From: $269 Sizes: 5 mgCatalog #: 90003

    5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH (4-9).

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    5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH (4-9).