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Amine-Reactive Dyes

Succinimidyl esters (SE, also known as NHS esters) react with primary amines such as lysine residues in proteins. SE dyes are commonly used for labeling proteins and antibodies. The reaction requires basic pH for target amines to be reactive, and is usually performed in pH 8.3 sodium bicarbonate buffer.

We offer more than 25 colors of our bright and photostable CF® Dye SE and easy-to-use CF® Dye Labeling Kits, as well as a wide variety of other amine-reactive dyes and biotinylation reagents below. Biotium’s CF® dye SE are more resistant to hydrolysis than other SE, resulting in excellent reaction efficiency.

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    AMCA-X, SE

    From: $139 Sizes: 10 mgCatalog #: 90077

    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

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    AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

     

    Carboxy-2′,7′- dichlorofluorescein diacetate, succinimidyl ester, 5 and 6 isomers

    From: $139 Sizes: 25 mgCatalog #: 90040

    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

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    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

     

    Carboxy-X-rhodamine free acid, 6-isomer

    From: $116 Sizes: 10 mgCatalog #: 90013

    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

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    6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

     

    Carboxy-X-rhodamine succinimidyl ester, 5 and 6 isomers

    From: $139 Sizes: 25 mgCatalog #: 90005

    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

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    5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.

     

    Carboxy-X-rhodamine succinimidyl ester, 5 isomer

    From: $162 Sizes: 5 mgCatalog #: 90036

    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

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    5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

     

    Carboxy-X-rhodamine succinimidyl ester, 6-isomer

    From: $162 Sizes: 5 mgCatalog #: 90037

    6-ROX, SE is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

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    6-ROX, SE is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

     

    Carboxyrhodamine 110 succinimidyl ester, 5 and 6 isomers

    From: $210 Sizes: 5 mgCatalog #: 90001

    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

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    5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

     

    Carboxyrhodamine 110 succinimidyl ester, 5-isomer

    From: $436 Sizes: 5 mgCatalog #: 90030

    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

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    Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

     

    Carboxyrhodamine 110 succinimidyl ester, 6-isomer

    From: $436 Sizes: 5 mgCatalog #: 90031

    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

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    Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

     

    Carboxyrhodamine 110-PEO12, SE, 5 isomer

    From: $127 Sizes: 1 mgCatalog #: 90086

    5-CR110-PEO12 SE (full name: 5-Carboxyrhodamine 110-PEO12-propionate, succinimidyl ester, single isomer) has a long, water-soluble PEG spacer between the green fluorescent rhodamine dye and the amine-reactive SE group.

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    5-CR110-PEO12 SE (full name: 5-Carboxyrhodamine 110-PEO12-propionate, succinimidyl ester, single isomer) has a long, water-soluble PEG spacer between the green fluorescent rhodamine dye and the amine-reactive SE group.

     

    Carboxyrhodamine 110-PEO4, SE, 5 isomer

    From: $386 Sizes: 5 mgCatalog #: 90081

    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

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    5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

     

    Carboxyrhodamine 110-PEO8, SE, 5 isomer

    From: $116 Sizes: 1 mgCatalog #: 90084

    5-CR110-PEO8 SE (full name: 5-Carboxyrhodamine 110-PEO8-propionate, succinimidyl ester, single isomer ) is similar to 5-CR110-PEO4 SE (90081) except that it has a longer PEG spacer.

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    5-CR110-PEO8 SE (full name: 5-Carboxyrhodamine 110-PEO8-propionate, succinimidyl ester, single isomer ) is similar to 5-CR110-PEO4 SE (90081) except that it has a longer PEG spacer.

     

    Carboxyrhodamine 110-X succinimidyl ester, 5 and 6 isomers

    From: $231 Sizes: 5 mgCatalog #: 90003

    5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH (4-9).

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    5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH (4-9).

     

    Carboxyrhodamine 110-X succinimidyl ester, 5-isomer

    From: $436 Sizes: 5 mgCatalog #: 90014

    5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer.

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    5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer.

     

    Carboxyrhodamine 110-X succinimidyl ester, 6-isomer

    From: $436 Sizes: 5 mgCatalog #: 90015

    6-CR110-X, SE (full name: 6-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 6-carboxyrhodamine 110 with a 6-carbon spacer.

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    6-CR110-X, SE (full name: 6-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 6-carboxyrhodamine 110 with a 6-carbon spacer.

     

    Carboxyrhodamine 6G succinimidyl ester, 5-isomer

    From: $162 Sizes: 5 mgCatalog #: 90032

    Amine-reactive form of 5-carboxyrhodamine 6G single isomer.

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    Amine-reactive form of 5-carboxyrhodamine 6G single isomer.