Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
The Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) is comparable to our original Steady-Luc™ HTS Assay (catalog no. 30028), but with assay buffer provided in lyophilized format for economical shipping (at toom temperature) and convenient storage (at -20°C). Simply dissolve the lyophilized assay buffer in the supplied reconstitution buffer before use.
- Steady-Luc Firefly Assay Buffer (Lyophilized)
- Reconstitution Buffer
- Simple: single step homogeneous assay.
- Glow kinetics: luminescence half life of about 3 hours.
- Convenient: lyophilized assay buffer for convenient shipping and storage.
- Economical: save up to 45% compared to other brands.
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