This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. This kit is a flash-type luminescence assay, which requires luminescence to be measured immediately after adding reagents to your sample.
This kit is a new and improved replacement for the Renilla Luciferase Assay Kit (catalog no. 30004). In addition to providing a simplified protocol, the Renilla Luciferase Assay Kit 2.0 features Biotium’s Aquaphile™ Coelenterazine, a water soluble substrate that can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine.
The stated number of assays is based on 96-well assay format. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (catalog no. 99912) can be purchased separately (see below).
Biotium also offers the Firefly & Renilla Luciferase Single Tube Assay Kit (catalog no. 30081), a combined luciferase assay kit for sequential measurement of Firefly and Renilla luciferase activity sequentially in the same sample with high sensitivity and linearity.
- 5X Passive Lysis Buffer
- Renilla Luciferase Assay Buffer 2.0
- Aquaphile™ Coelenterazine
More Luciferase Kits
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