Reactive Oxygen Species Indicators

Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.

Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.

Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 (10055) but with increased stability toward air oxidation and light during storage.

H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.

Methyl coelenterazine (Coelenterazine, 2-methyl analog) has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion (1).