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Cell Surface & Membrane Dyes

Biotium offers a variety of cell surface stains that depend on the workflow and application. CytoLiner™ Fixed Cell Membrane Stains are novel lipophilic fluorescent dyes designed for selective and robust labeling of the plasma membrane in formaldehyde-fixed cells.

CellBrite® Fix and MemBrite® Fix Membrane Staining Kits offer stable covalent labeling of cell surfaces and can withstand fixation and permeabilization. These stains are designed to be used on live cells that will be fixed shortly after.

CellBrite® Steady Membrane Staining Kits were designed specifically for staining cell surfaces in live cells for long-term imaging. The stains are retained on the cell surface, unlike other membrane dyes that are rapidly internalized.

We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes, which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking.

Learn more about our cell membrane stains and which is right for you.

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CellBrite® Fix Membrane Stains

From: $171 Sizes: Trial size (1 vial), Set of 5 vialsCatalog #:
30089, 30089-T, 30088, 30088-T, 30090, - 30090-TView allHide

Unique fluorogenic membrane dyes that covalently stain the plasma membrane in live cells, and can withstand both fixation and detergent permeabilization.

MemBrite® Fix Cell Surface Staining Kits

From: $197 Sizes: 100 labelings, 500 labelingsCatalog #:
30092, 30092-T, 30093, 30093-T, 30094, 30094-T, 30095, 30095-T, 30096, 30096-T, 30097, 30097-T, 30098, 30098-T, 30099, 30099-T, 30101, 30101-T, 30102, 30102-T, 30103, 30103-T, 30104, - 30104-TView allHide

Wide choice of dye colors for covalent staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments.

CellBrite® Cytoplasmic Membrane Dyes

From: $165 Sizes: 1 mL (200X in EtOH), 100 uL (2 mM in DMSO), 50 assaysCatalog #:
30079, 30078, 30077, 30070, 30021, 30022, 30023, - 30024View allHide

Dye solutions of lipophilic carbocyanine dyes DiB, Neuro-DiO, DiI, and DiD, as well as novel near-infrared lipophilic dyes for non-toxic labeling of cytoplasmic membranes.

Concanavalin A (Con A) CF® Dye Conjugates

From: $196 Sizes: 5 x 1 mgCatalog #:
29136, 29015, 29016, 29017, 29018, 29019, 29020, 29058, 29074, 29075, - 29080View allHide

A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

CellBrite® Steady Membrane Staining Kits

From: $244 Sizes: 100 labelings, 500 labelingsCatalog #:
30105-T, 30105, 30106-T, 30106, 30107-T, 30107, 30108-T, 30108, 30109-T, - 30109View allHide

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

CytoLiner™ Fixed Cell Membrane Stains

From: $390 Sizes: 250 labelings, 1000 labelingsCatalog #:
30140, 30140-T, 30131-T, 30131, 30132-T, 30132, 30133-T, 30133, 30134-T, 30134, 30135-T, - 30135View allHide

Novel lipophilic fluorescent dyes for selective staining of the plasma membrane in formaldehyde-fixed cells. Available in 6 colors, from blue to near-IR.

Datura Stramonium Lectin (DSL) Conjugates

From: $190 Sizes: 1 mLCatalog #:
29131, 29096, 29097, 29098, 29099, 29100, - 29101View allHide

DSL binds to (beta-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over single N-acetylglucosamine residues. Biotium offers DSL conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) Conjugates

From: $190 Sizes: 1 mLCatalog #:
29132, 29102, 29103, 29104, 29105, 29106, - 29107View allHide

Tomato lectin (LEL, TL) CF® Dye conjugates that bind to [GlcNAc] 1,3-N-acetylglucosamine, glycophorin, and Tamm-Horsfall glycoprotein. LEL, TL is a commonly used marker for blood vessels and microglial cells in rodent and neuroscience research. Biotium offers tomato lectin conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

Phaseolus Vulgaris Leucoagglutinin (PHA-L) Conjugates

From: $180 Sizes: 1 mLCatalog #:
29134, 29114, 29115, 29116, 29117, 29118, - 29119View allHide

PHA-L triggers lymphocyte agglutination and is available conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

Sambucus Nigra Lectin (SNA, EBL) Conjugates

From: $215 Sizes: 1 mLCatalog #:
29135, 29120, 29121, 29122, 29123, 29124, - 29125View allHide

SNA lectin binds to sialic acid attached to terminal galactose. SNA lectin is a carbohydrate-binding protein that can induce apoptosis in insect cells and cultured mammalian cell cancer cells. Biotium offers SNA lectin conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

Ulex Europaeus Agglutinin I (UEA I) Conjugates

From: $180 Sizes: 1 mLCatalog #:
29133, 29108, 29109, 29110, 29111, 29112, - 29113View allHide

UEA I binds to glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. The lectin is an established marker for human endothelial cells and has been used to differentiate between angiosarcomas and epithelial tumors. Biotium offers UEA I conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

Wheat Germ Agglutinin (WGA) Conjugates

From: $87 Sizes: 1 mg, 5 x 1 mgCatalog #:
29128-1, 29128, 29095-1, 29095, 29021, 29022, 29023, 29024, 29025, 29026, 29027, 29028, 29029, 29059, 29064, 29073, 29021-1, 29022-1, 29023-1, 29024-1, 29025-1, 29026-1, 29027-1, 29028-1, 29029-1, 29059-1, 29064-1, 29077-1, 29077, 29076-1, - 29076View allHide

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes, biotin, or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

1,6-Diphenyl-1,3,5-hexatriene

From: $71 Sizes: 100 mgCatalog #: 60021

1,6-Diphenyl-1,3,5-hexatriene (DPH) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.

Biotin-DHPE

From: $276 Sizes: 10 mgCatalog #: 60022

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-X-DHPE

From: $276 Sizes: 5 mgCatalog #: 60023

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

DiA

From: $219 Sizes: 50 mgCatalog #: 60013

DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI (60010) have been used together for two color membrane staining.