Cell Surface & Membrane Dyes

CellBrite™ Fix Membrane Stains are a new class of fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in that they can withstand both fixation and detergent permeabilization. Available with green, red, and far-red fluorescence.

MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.

Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.

This product is concanavalin A (Con A) labeled with our superior fluorescent CF® dyes. Con A is a widely used lectin that selectively binds to a-mannopyranosyl and a-glucopyranosy residues, found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells.

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.

1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI (60010) have been used together for two color membrane staining.

DiB is a lipophilic cationically charged blue fluorescent dye useful for staining cytoplasmic membranes. λEx/λEm (MeOH) = 353/442 nm.

DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths. DiR, DiD, DiI and Neuro-DiO can be used in combination for multicolor imaging.

DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons.

DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Carbocyanine microinjection has been widely used for fate mapping and cell lineage studies and for the determination of spatial organization and connectivity patterns of central nervous systems.

Dilinoleyl DiI (1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI, has identical absorption and emission spectra as DiI (#60010) .

Dilinoleyl DiO (1,1′-dilinoleyl-3,3′-oxacarbocynanine perchlorate), also known as FAST DiO, has identical absorption and emission spectra as DiO (#60011).