Cell Surface & Membrane Dyes
Biotium offers two new classes of dyes for covalent labeling of the cell surface that are unique in that they can withstand fixation and permeabilization for subsequent IF staining. CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. MemBrite™ Fix dyes covalently label cell surface proteins, and are available in with a wide selection of bright and photostable colors.
We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes, which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking.
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MemBrite™ Fix Cell Surface Staining Kits
MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.
MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.
CellBrite™ Fix Membrane Stains
CellBrite™ Fix Membrane Stains are a new class of fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in that they can withstand both fixation and detergent permeabilization. Available with green, red, and far-red fluorescence.
CellBrite™ Fix Membrane Stains are a new class of fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in that they can withstand both fixation and detergent permeabilization. Available with green, red, and far-red fluorescence.
CellBrite™ Cytoplasmic Membrane Dyes
CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.
CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.
CellBrite™ NIR Cytoplasmic Membrane Dyes
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.
1,6-Diphenyl-1,3,5-hexatriene
1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.
1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.
Nile Red
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments.
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments.
DiOC5(3)
DiOC5(3) and DiOC6(3) are two of the most widely used carbocyanine dyes used for membrane potential measurements.
DiOC5(3) and DiOC6(3) are two of the most widely used carbocyanine dyes used for membrane potential measurements.
RH795
The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not (see references 1,2).
The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not (see references 1,2).
RH237
The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. Excitation/emission data (below) is for the dye in methanol.
The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. Excitation/emission data (below) is for the dye in methanol.
RH421
RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.
RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.
DiOC16(3)
DiOC16(3) is a lipophilic cyanine dye with two C16 hydrocarbon chains. It can be optimally excited by the 488 nm argon laser line with green fluorescence emission (λEx/λEm = 484/501 nm).
DiOC16(3) is a lipophilic cyanine dye with two C16 hydrocarbon chains. It can be optimally excited by the 488 nm argon laser line with green fluorescence emission (λEx/λEm = 484/501 nm).
Dipicrylamine
Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes.
Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes.
DiB
DiB is a lipophilic cationically charged blue fluorescent dye useful for staining cytoplasmic membranes. λEx/λEm (MeOH) = 353/442 nm.
DiB is a lipophilic cationically charged blue fluorescent dye useful for staining cytoplasmic membranes. λEx/λEm (MeOH) = 353/442 nm.
Octadecyl Rhodamine B chloride (R18)
The fluorescence of octadecyl rhodamine B in membranes is quenched at high dye concentration but is released at dilution. This property makes the dye useful for membrane fusion assays.
The fluorescence of octadecyl rhodamine B in membranes is quenched at high dye concentration but is released at dilution. This property makes the dye useful for membrane fusion assays.
NBD C6-Sphingomyelin
NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.
NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.
NBD C6-Ceramide
NBD C6-ceramide (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine) has been used for following sphingolipid metabolism in cells, and as a selective stain for the Golgi apparatus in live and fixed cells.
NBD C6-ceramide (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine) has been used for following sphingolipid metabolism in cells, and as a selective stain for the Golgi apparatus in live and fixed cells.