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EXTRACELLULAR VESICLE RESEARCH

Annexin V conjugates optimized for staining purified EVs from a broad range of sources
Wheat germ agglutinin (WGA) conjugates optimized for staining purified or bead-bound EVs from a broad range of sources
Cholera toxin subunit B (CTB) conjugates optimized for staining purified or bead-bound EVs
Validated antibodies for detection of EV markers in purified or bead-bound EVs by flow cytometry
Streptavidin-conjugated magnetic beads validated for isolation of EVs when combined with a biotinylated antibody
ExoBrite™ STORM CTB EV Stains were developed for super-resolution dSTORM imaging of isolated EVs
Validated antibodies for detection of EV markers in EV extracts by western blot

What Are Extracellular Vesicles?

Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. There are several subtypes of EVs that vary in function, cargo, and size, which can range from ~30-1000 nm in diameter. The smallest type of EV is the exosome, which is ~30-150 nm in size. EVs originate within a cellular compartment called the multivesicular body (MVB), which is itself derived from invagination of endosomes. The vesicles are released when the MVB fuses with the plasma membrane and releases its cargo.

In biomedical research, EVs and their cargo are used as diagnostic biomarkers for cancer and other diseases. EVs can be isolated from blood or other biological fluids using techniques such as ultracentrifugation, PEG precipitation, and immuno-capture beads.

EV membranes harbor transmembrane proteins originating from the plasma membrane of the cell of origin, which often includes proteins of the tetraspanin family (such as CD9, CD63, and CD81). Inside, EVs contain cytoplasmic components such as proteins and RNA. EV components can be analyzed using methods like RNAseq, western blotting, and flow cytometry. For characterization by flow cytometry, researchers can use dyes that stain EV components like membranes and nucleic acids, and antibodies that bind to tetraspanins or other proteins of interest.

Read our blog article to to learn more about EVs and their growing potential in both medicine and research.

 

EV analysis: Small targets, big challenge

The small size of EVs makes them challenging to handle and analyze. In flow cytometry, EVs can be difficult to distinguish from cell debris and other small particles, with their size at or below the limits of some flow cytometers’ sensitivity. Staining with certain dyes or antibodies can help to distinguish EVs from other particles. However, it is important to use stains that won’t form aggregates, since these small particles could themselves be confused for stained EVs. Such aggregates have been a frustration for EV and exosome researchers.

The method used to enrich or purify EVs prior to analysis is also an important factor. Samples enriched with a simple PEG precipitation step often contain a large amount of non-EV lipid particles, which may also bind to membrane dyes. These impurities can be reduced by using other EV purification methods, such as size exclusion chromatography (SEC) or immunoprecipitation. Magnetic beads bound to anti-tetraspanin antibodies can give pure EV clusters. However, these beads may bind non-specifically with a lot of hydrophobic dyes, such as membrane dyes.

We have screened a large collection of our antibodies and dyes to look for those that both stain EVs well, and show minimal to no aggregation (i.e, signal in the absence of EVs). Continue reading below to explore our validated EV antibodies and dyes.

Check Out Our helpful Tech Tips on EV Isolation and Detection

Tech Tip: Fluorescent Detection of Exosomes by Flow Cytometry

In this Tech Tip, we share the expertise we have acquired for optimal fluorescent staining and detection of EVs.

Tech Tip: Exosome Isolation and Staining Protocols

In this Tech Tip, Biotium scientists provide detailed protocols for isolating EVs from cell supernatants using PEG precipitation or size exclusion chromatography (SEC).

EXOBRITE™ EV SURFACE STAINING KITS

Detect EVs, Not Dye Aggregates

Membrane dyes, while common tools for labeling EVs, can pose significant challenges when used for EV staining. For example, membrane dyes such as PKH, DiO, and DiI, have poor solubility and thus form aggregates that can be confused with EVs. To overcome these challenges, Biotium developed ExoBrite™ EV Surface Stains, which provide bright staining of isolated EVs with minimal dye aggregation.

ExoBrite™ EV Surface Stains are conjugates of probes (Annexin V, WGA, and CTB) that are developed and validated for bright and specific staining of EV surface targets with minimal aggregation in flow cytometry. In addition, ExoBrite™ EV Surface Stains are compatible with antibody co-staining and do not bind non-specifically to polystyrene beads, and therefore unlike hydrophobic membrane dyes, they can be used to stain bead-bound EVs. For flexible panel design, ExoBrite™ EV Surface Stains are available in four colors for the Pacific Blue™, FITC, PE, and APC channels.

Advantages of ExoBrite™ EV Surface Stains:

  • Available as ExoBrite™ Annexin V, CTB, and WGA conjugates
  • Optimized for bright and sensitive EV staining by flow cytometry
  • Stain purified or bead-bound EVs
  • Compatible with antibody co-staining
  • Available in 4 colors for flexible panel design
  • Sampler kit with Annexin V, CTB, and WGA stains available

ExoBrite™ EV Surface Staining Kits

ProductConjugateDetection channelsSizeCatalog Number
ExoBrite™ Annexin EV Staining KitsAnnexin VPacific Blue™, FITC, PE, Cy®3, APC100 Labelings, 500 Labelings30119-30122
ExoBrite™ WGA EV Staining KitsWheat Germ Agglutinin (WGA)30123-30126
ExoBrite™ CTB EV Staining KitsCholera Toxin Subunit B (CTB)30111-30114
ExoBrite™ EV Surface Stain Sampler Kit, GreenAnnexin V, Wheat Germ Agglutinin (WGA), Cholera Toxin Subunit B (CTB)FITC100 Labelings30127
ExoBrite™ STORM CTB EV Staining KitsCholera Toxin Subunit B (CTB)FITC, Texas Red®, Cy®5, Cy®5.5100 Labelings, 500 Labelings30115-30118
SEC purified EVs stained with three different ExoBrite™ stains: ExoBrite™ 560/585 Annexin Stain, ExoBrite™ 490/515 WGA Stain, and ExoBrite™ 410/450 CTB Stain.

Validated EV Sources for ExoBrite™ EV Surface Stains

EV SourceExoBrite™ CTB StainsExoBrite™ WGA StainsExoBrite™ Annexin Stains
A549 cellsYesYesYes
CHO cellsNoYesYes
hASC (human adipose stem cells)No1NDND
HeLa cellsNoYesYes
HUVEC (human umbilical vein endothelial cells)No1NDND
J774 cellsYesYesYes
Jurkat cellsYesYesYes
MCF-7 cellsYesYesYes
PlasmaNoNDYes
Raji cellsYesYesYes
SerumNoNDYes
Skeletal myoblastsYes1NDND
U2OS cellsNoYesYes
U937 cellsNoYesYes
1Customer-reported data
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.

Less background and better coverage over other EV stains

ExoBrite™ CTB EV Stains have less background and more complete staining of extracellular vesicles (EVs) than other classic and competitor dyes. EVs were purified from MCF-7 cell supernatant using size exclusion chromatography (SEC). The purified EVs were stained in PBS with the indicated dyes (top row). The stained EV population was gated, with the number showing the percentage of particles falling within the exosome gate. Each dye was also added to filtered PBS (bottom row), to look for dye aggregation and non-specific background. Red arrows indicate these dye aggregates. Lipophilic dyes like DiO show a high number of particles of similar size to EVs, making them unsuited for small particle staining. CellMask™ also shows an unacceptable amount of dye aggregation falling within the EV gate. The ExoFlow-ONE™ and ExoGlow™ dyes form aggregates that can mostly be gated away from the EVs, but they also show less-complete coverage of EVs than ExoBrite™ CTB stains (Click to enlarge).

EXOBRITE™ ANNEXIN EV STAINING KITS

Bright & Sensitive EV Stains with Broad Compatibility

ExoBrite™ Annexin EV Staining Kits are uniquely formulated conjugates of Annexin V designed with broad compatibility for EVs derived from different sources. Similar to the ExoBrite™ CTB EV Stains, these Annexin V-based conjugates were developed for bright and sensitive staining of isolated EVs by flow cytometry with little to no background aggregation. ExoBrite™ Annexin EV Staining Kits also offer the broadest coverage for EVs. We tested EVs derived from 9 cell lines and ExoBrite™ Annexin EV Stains showed strong staining for all of them.

Advantages of ExoBrite™ Annexin EV Stains:

  • Optimally formulated Annexin V conjugates for staining purified EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Bright signal and low background staining for flow cytometry
  • Compatible with antibody co-staining
  • Available in 4 colors for flexible experimental design
ExoBrite™ Annexin EV Staining Kits
SEC-purified HeLa-derived EVs were stained with ExoBrite™ Annexin EV Stains.
ExoBrite™ 490/515 Annexin EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD9 in 100 uL, followed by 1X ExoBrite™ 655/670 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 655/670 WGA-positive particles, ~50% were also positive for CD9.

ExoBrite™ Annexin EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
Annexin EV Staining Kit
416/452 nmPacific Blue™100 Labelings30119-T
500 Labelings30119
ExoBrite™ 490/515
Annexin EV Staining Kit
490/516 nmFITC100 Labelings30120-T
500 Labelings30120
ExoBrite™ 560/585
Annexin EV Staining Kit
562/584 nmPE100 Labelings30121-T
500 Labelings30121
ExoBrite™ 655/670
Annexin EV Staining Kit
652/668 nmAPC100 Labelings30122-T
500 Labelings30122

EXOBRITE™ WGA EV STAINING KITS

Sensitive Stains for Purified or Bead-Bound EVs with Broad Compatibility

ExoBrite™ WGA EV Stains are fluorescent conjugates of wheat germ agglutinin (WGA) optimized for bright and sensitive staining of EVs. Similar to ExoBrite™ Annexin EV Stains, these WGA-based conjugates offer broad compatibility with EVs. The stains were validated for EVs derived from all 9 cell lines tested. In addition, ExoBrite™ WGA EV Stains are less prone to aggregation than hydrophobic membrane dyes and may be used to stain purified and bead-bound EVs.

Advantages of ExoBrite™ WGA EV Stains:

  • Optimally formulated WGA conjugates for staining purified or bead-bound EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Bright signal and low background staining for flow cytometry
  • Compatible with antibody co-staining
  • Available in 4 colors for flexible experimental design
ExoBrite™ WGA EV Staining Kits
SEC-purified EVs were stained with ExoBrite™ WGA EV Stains. MCF-7 derived EVs were stained with ExoBrite™ 410/450, ExoBrite™ 490/515, or ExoBrite™ 560/585. CHO derived EVs were stained with ExoBrite™ 640/660. 410/450 was detected in the Pacific Blue channel, 490/515 was detected in the FITC channel, 560/585 was detected in the PE channel, and 640/660 was detected in the APC channel.
ExoBrite™ 490/515 WGA EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified MCF-7-derived EVs were stained first with ExoBrite™ 490/515 CD81 in 100 uL, followed by 1X ExoBrite™ 640/660 WGA EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the FITC and APC channels. When we gated on ExoBrite™ 640/660 WGA-positive particles, ~60% were also positive for CD81.

ExoBrite™ WGA EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
WGA EV Staining Kit
416/452 nmPacific Blue™100 Labelings30123-T
500 Labelings30123
ExoBrite™ 490/515
WGA EV Staining Kit
490/516 nmFITC100 Labelings30124-T
500 Labelings30124
ExoBrite™ 560/585
WGA EV Staining Kit
562/584 nmPE100 Labelings30125-T
500 Labelings30125
ExoBrite™ 640/660
WGA EV Staining Kit
642/663 nmAPC100 Labelings30126-T
500 Labelings30126

EXOBRITE™ CTB EV STAINING KITS

Bright EV Stains with Excellent Signal-to-Noise

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection. ExoBrite™ stains bind to molecules in the EV membrane providing bright, specific staining of isolated EVs by flow cytometry. A key advantage of ExoBrite™ stains is that, unlike many other dyes, they show little to no background aggregates of a similar size as EVs. Also, unlike most membrane dyes, ExoBrite™ stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound EVs.

Advantages of ExoBrite™ CTB EV Stains:

  • Optimally formulated CTB conjugates for staining EVs
  • Designed for detection by flow cytometry
  • Bright fluorescence and low background for excellent signal-to-noise
  • Compatible with antibody co-staining
  • Stain purified or bead-bound EVs
  • Available in 4 colors for flexible experimental design
ExoBrite™ CTB EV Staining Kits
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ CTB EV Stains.
ExoBrite™ 490/515 CTB EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified MCF-7-derived EVs were stained first with ExoBrite™ 490/515 CD9 in 100 uL, followed by 10X ExoBrite™ 560/585 CTB EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the FITC and R-PE channels. When we gated on ExoBrite™ 560/585 CTB-positive particles, ~95% were also positive for CD9.

ExoBrite™ CTB EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
CTB EV Staining Kit
416/452 nmPacific Blue™100 Labelings30111-T
500 Labelings30111
ExoBrite™ 490/515
CTB EV Staining Kit
490/516 nmFITC100 Labelings30112-T
500 Labelings30112
ExoBrite™ 560/585
CTB EV Staining Kit
562/584 nmPE, Cy®3100 Labelings30113-T
500 Labelings30113
ExoBrite™ 640/660
CTB EV Staining Kit
642/663 nmAPC100 Labelings30114-T
500 Labelings30114

CellMask is a trademark of Thermo Fisher Scientific; ExoFlow-ONE and ExoGlow are trademarks of System Biosciences.

ExoBrite™ Flow Antibody Conjugates

Validated antibodies for detection of EV markers by flow cytometry

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. ExoBrite™ Flow Antibody Conjugates were designed and validated for flow cytometry to offer bright signal and low background of EV markers in purified and bead-bound EVs.

ExoBrite™ Isotype Control Flow Antibody, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.

Advantages of ExoBrite™ Flow Antibodies:

  • Developed for detection of EV markers CD9, CD63, and CD81 by flow cytometry
  • Validated for purified or bead-bound EVs
  • Bright signal and low background
  • ExoBrite™ Isotype Control Flow Antibody available
  • Colors available for Pacific Blue™, FITC, and PE channels

Purified EVs stained with ExoBrite™ Flow Antibodies

SEC-purified A549-derived EVs were stained with ExoBrite™ 650/665 CD9 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the APC channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the PE channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD81 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Pacific Blue channel.

ProductConjugatesDetection ChannelsSizesCatalog Number
ExoBrite™ CD9 Flow AntibodyExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
ExoBrite™ R-PE
Pacific Blue ™
FITC
PE
25 tests
100 tests
P003-410… P003-RPE
ExoBrite™ CD63 Flow AntibodyP004-410… P004-RPE
ExoBrite™ CD81 Flow AntibodyP005-410… P005-RPE
ExoBrite™ IgG1 Isotype Control Flow AntibodyP008-410… P008-RPE

ExoBrite™ Streptavidin Magnetic Beads

Convenient & Optimized Capture of EVs

Streamline your EV and exosome isolation workflows with ExoBrite™ Streptavidin Magnetic Beads, developed to offer lower background and higher sensitivity for EV capture than similar products. These streptavidin-coated and magnetic polystyrene beads (4.5 um diameter) may be used to isolate EVs from cell culture medium or other biological fluids without an overnight precipitation step.

ExoBrite™ Streptavidin Magnetic Beads

Advantages of ExoBrite™ Streptavidin Magnetic Beads:

  • Combine with a biotinylated antibody (not included) for isolation of EVs
  • Isolate EVs from biofluids without an overnight precipitation step
  • Lower background and higher sensitivity than competitors
  • Convenient magnetic-based separation
  • Detect downstream with microscopy, flow cytometry, or western blot
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD9 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD63 antibody, and then with concentrated MCF-7 cell supernatant. Bound EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.

 

ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CD9 Flow Antibody and were detected by flow cytometry in the PE channel.
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.

SUPER-RESOLUTION IMAGING

See beyond the diffraction limit with
ExoBrite™ STORM CTB EV Staining Kits

Characterizing exosomes and EVs by imaging remains a challenge due to their small size and the resolution limit of light microscopy. Super-resolution microscopy techniques such as direct stochastic optical reconstruction microscopy (dSTORM) push beyond the diffraction limit of traditional light microscopy, allowing single-molecule resolution of subcellular structures such as EVs.

ExoBrite™ STORM CTB EV Stains were developed for STORM imaging of EVs and are available in four CF® Dyes validated for STORM.  ExoBrite™ 560/585 has been validated for dSTORM on the ONI Nanoimager S Mark II, allowing the study of fine morphological details and co-staining with EV biomarkers such as tetraspanin proteins CD9, CD63, and CD81.

Advantages of ExoBrite™ STORM CTB EV Stains:

  • Dive deeper into EV morphology and structure
  • Optimally formulated CTB conjugates for imaging EVs by STORM
  • Available in 4 CF® Dyes validated for STORM imaging
  • Allows antibody co-staining and localization studies with EV biomarkers
ExoBrite™ STORM CTB EV Staining Kits
Super-resolution dSTORM images of human colorectal cancer cell line derived EVs. EVs were stained with anti-tetraspanin antibodies (an anti-CD9/CD63/CD81 cocktail, all conjugated to CF®647, shown in magenta) together with 1X ExoBrite™ 560/585 (in cyan). Samples were prepared using ONI’s EV Profiler Kit and acquired using the Nanoimager S Mark II from ONI (Oxford Nanoimaging, UK). Data was processed using a beta-release version of CODI, ONI’s cloud-based data analysis platform. Scale bars are 500 nm (zoomed out, left panel) and 50 nm for single-EV panels (right). Image courtesy of ONI.

ProductEx/Em (nm)Laser Line(s) (nm)Detection ChannelSizeCatalog Number
ExoBrite™ STORM CF®505 CTB EV Staining Kit505/519488FITC100 Labelings30115-T
500 Labelings30115
ExoBrite™ STORM CF®583R CTB EV Staining Kit583/609555 or 561Rhodamine or
Texas Red®
100 Labelings30116-T
500 Labelings30116
ExoBrite™ STORM CF®647 CTB EV Staining Kit652/668633-640Cy®5100 Labelings30117-T
500 Labelings30117
ExoBrite™ STORM CF®680 CTB EV Staining Kit681/698633-640Cy®5.5100 Labelings30118-T
500 Labelings30118

ExoBrite™ Western Antibody Conjugates

Validated antibodies for detection of EV markers by western blotting

ExoBrite™ Western Antibodies were validated to offer bright signal and low background of EV markers CD9, CD63, and CD81 in EV extracts by near-IR fluorescent western blot. The antibodies are available with two near-infrared fluorescent dyes, ExoBrite™ 680/700 and ExoBrite™ 770/800, which offer greater signal-to-noise than dyes with visible light emission for western blotting.

An ExoBrite™ Calnexin Western Antibody detects a protein of the endoplasmic reticulum that is not found in EVs. It is offered as a negative control to assess the purity of isolated EV extracts.

Advantages of ExoBrite™ Western Antibodies:

  • Developed for detection of EV markers CD9, CD63, and CD81 by fluorescent western blot
  • Validated for use with EV extracts
  • Bright signal and low background
  • Available in 2 near-infrared colors
  • Negative control ExoBrite™ Calnexin Western Antibody available

Western detection of human CD9 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD9 Western Antibody, showing enrichment of CD9 in the EV prep.
Western detection of human CD63 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD63 Western Antibody, showing enrichment of CD63 in the EV prep.
Western detection of human CD81 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD81 Western Antibody, showing enrichment of CD81 in the EV prep.
Western detection of human calnexin in MCF-7 cell and EV lysate using ExoBrite™ 770/800 calnexin Western Antibody, showing the lack of calnexin in the EV prep.

ProductConjugatesSizesCatalog Number
ExoBrite™ CD9 Western AntibodyExoBrite™ 680/700
ExoBrite™ 770/800
25 tests
100 tests
P003-680, P003-770
ExoBrite™ CD63 Western AntibodyP004-680, P004-770
ExoBrite™ CD81 Western AntibodyP006-680, P006-770
ExoBrite™ Calnexin Western AntibodyExoBrite™ 770/800P007-770

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