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EXTRACELLULAR VESICLE RESEARCH

Lipophilic dyes designed as a superior alternative to PKH, DiO, DiI, DiD for pan-EV labeling
Annexin V conjugates optimized for staining purified EVs from a broad range of sources
Wheat germ agglutinin (WGA) conjugates optimized for staining purified or bead-bound EVs from a broad range of sources
Cholera toxin subunit B (CTB) conjugates optimized for staining purified or bead-bound EVs
Validated antibodies for detection of EV markers in purified or bead-bound EVs by flow cytometry
Streptavidin-conjugated magnetic beads validated for isolation of EVs when combined with a biotinylated antibody
ExoBrite™ STORM CTB EV Stains were developed for super-resolution dSTORM imaging of isolated EVs
Validated antibodies for detection of EV markers in EV extracts by western blot
Optimized kit for extracting total RNA, including mRNA and miRNA, from purified EVs

Check Out Our helpful Tech Tips on EV Isolation and Detection

Tech Tip: Fluorescent Detection of Exosomes by Flow Cytometry

In this Tech Tip, we share the expertise we have acquired for optimal fluorescent staining and detection of EVs.

Tech Tip: Exosome Isolation and Staining Protocols

In this Tech Tip, Biotium scientists provide detailed protocols for isolating EVs from cell supernatants using PEG precipitation or size exclusion chromatography (SEC).

Robust Stains Designed to Detect EVs, Not Dye Aggregates

Membrane dyes, while common tools for labeling EVs, can pose significant challenges when used for EV staining. For example, membrane dyes such as PKH, DiO, and DiI, have poor solubility and thus form aggregates that can be confused with EVs. To overcome these challenges, Biotium developed ExoBrite™ True EV Membrane Stains and ExoBrite™ EV Surface Stains (Annexin V, WGA, and CTB) to offer higher coverage and signal-to-noise over membrane dyes commonly used for EV staining. View our comparison table and validated EV sources below to select the best stain for your next experiment.

ExoBrite™ True EV Membrane Stains are lipophilic dyes designed as the best choice for pan-EV labeling

SEC purified EVs stained with ExoBrite™ 515/540 True EV Membrane Stain and ExoBrite™ 555/575 True EV Membrane Stain.

ExoBrite™ CTB, WGA, and Annexin V Conjugates offer bright staining of EVs with broad compatibility and low background

SEC purified EVs stained with three different ExoBrite™ stains: ExoBrite™ 560/585 Annexin Stain, ExoBrite™ 490/515 WGA Stain, and ExoBrite™ 410/450 CTB Stain.

ExoBrite™ EV Stains Comparison Guide

ExoBrite™ EV Surface Stain Pros Cons
ExoBrite™ True EV Membrane Stains • Near-complete staining of EVs in a sample
• Broad compatibility with different EV sources
• Validated for flow and fNTA
• Can’t be used to stain bead-bound EVs
• May have more aggregation than CTB & Annexin
ExoBrite™ Annexin EV Staining Kits • Broad compatibility with different EV sources
• Validated for flow and fNTA
• Low background aggregates
• May not stain every EV in a sample
• Doesn’t work well on bead-bound EVs
ExoBrite™ WGA EV Staining Kits • Broad compatibility with different EV sources
• Can be used with bead-bound EVs
• May not stain every EV in a sample
• Doesn’t work well for fNTA
ExoBrite™ CTB EV Staining Kits • Validated for flow and fNTA
• Extremely low background
• May not stain every EV in a sample
• Does not stain EVs from every source
ExoBrite™ Antibodies • Highly specific for human tetraspanins CD9, CD63, or CD81
• Validated for EV flow
• Broad compatibility for different EV sources
• Can be used with bead-bound EVs
• Can be used for WB
• Depends on the expression level of the target protein on the EVs

ExoBrite™ EV Surface Stains

Product Conjugate Detection channels Size Catalog Number
ExoBrite™ True EV Membrane Stains N/A FITC, PE 100 Labelings, 500 Labelings 30129, 30130
ExoBrite™ Annexin EV Staining Kits Annexin V Pacific Blue™, FITC, PE, Cy®3, APC 100 Labelings, 500 Labelings 30119-30122
ExoBrite™ WGA EV Staining Kits Wheat Germ Agglutinin (WGA) 30123-30126
ExoBrite™ CTB EV Staining Kits Cholera Toxin Subunit B (CTB) 30111-30114
ExoBrite™ EV Surface Stain Sampler Kit, Green Annexin V, Wheat Germ Agglutinin (WGA), Cholera Toxin Subunit B (CTB) FITC 100 Labelings 30127
ExoBrite™ STORM CTB EV Staining Kits Cholera Toxin Subunit B (CTB) FITC, Texas Red®, Cy®5, Cy®5.5 100 Labelings, 500 Labelings 30115-30118

Validated EV Sources for ExoBrite™ EV Surface Stains

EV Source ExoBrite™ True EV Membrane Stains ExoBrite™ CTB Stains ExoBrite™ WGA Stains ExoBrite™ Annexin Stains
A549 cells Yes Yes Yes Yes
CHO cells Yes No Yes Yes
hASC (human adipose stem cells) ND No1 ND ND
HeLa cells Yes No Yes Yes
HUVEC (human umbilical vein endothelial cells) ND No1 ND ND
J774 cells Yes Yes Yes Yes
Jurkat cells Yes Yes Yes Yes
MCF-7 cells Yes Yes Yes Yes
Plasma ND No ND Yes
Raji cells ND Yes Yes Yes
RAW 264.7 Yes ND ND ND
Serum ND No ND Yes
Skeletal myoblasts ND Yes1 ND ND
THP-1 Yes ND ND ND
U2OS cells Yes No Yes Yes
U937 cells Yes No Yes Yes
1Customer-reported data
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.

EXOBRITE™ TRUE EV MEMBRANE STAINS

The Best Choice for Pan-EV Staining

ExoBrite™ True EV Membrane Stains are novel membrane dyes designed specifically for EV detection. While other membrane dyes like PKH, DiO, and DiI, are commonly used for labeling EVs, these dyes often have poor solubility and thus form aggregates that can be confused with EVs.

ExoBrite™ True EV Membrane Stains were designed specifically to address the issues of membrane dye aggregation and are the best choice for general pan-EV staining.  They demonstrate higher (near-complete) coverage of EVs over competitor membrane dyes in fluorescence nanoparticle tracking analysis (fNTA). They also offer clear differentiation of EVs from non-specific particles during flow detection, which is not usually possible with other membrane dyes.

 

Advantages of ExoBrite™ True EV Membrane Stains

  • Lipophilic membrane dyes designed for pan-EV labeling
  • Superior alternative to common membrane dyes like PKH, DiO, DiI, DiD
  • Bright signal with near-complete coverage of EVs in a sample
  • Broad compatibility with EVs isolated from different sources
  • Available in two colors for detection by flow in FITC or PE channels
  • Compatible with antibody co-staining

Discover Superior EV Coverage and Signal-to-Noise Over PKH, DiO, and Other Membrane Dyes

SEC purified MCF-7-derived EVs were stained with three green membrane dyes: ExoBrite™ 515/540 True EV Membrane Stain (left panel), PKH67 (middle panel), and Neuro-DiO (right panel). Of the three, only the ExoBrite™ True EV Membrane Stain is able to differentiate EVs from background particles in flow cytometry. The samples were run on a CytoFLEX LX flow cytometer in the FITC channel.
SEC purified MCF-7-derived EVs were stained with ExoBrite™ 515/540 True EV Membrane Stain, PKH67, and Neuro-DiO. The stained EVs were diluted and run on a ZetaView particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 488 nm excitation and 500 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 515/540 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than PKH67 or Neuro-DiO.

ExoBrite™ True EV Membrane Stains

Product Ex/Em Detection channels Size Catalog Number
ExoBrite™ 515/540 True EV Membrane Stain 515/542 nm FITC 100 Labelings 30129-T
500 Labelings 30129
ExoBrite™ 555/575 True EV Membrane Stain 556/576 nm PE 100 Labelings 30130-T
500 Labelings 30130

EXOBRITE™ ANNEXIN EV STAINING KITS

Bright & Sensitive EV Stains with Broad Compatibility

ExoBrite™ Annexin EV Staining Kits are uniquely formulated conjugates of Annexin V designed with broad compatibility for EVs derived from different sources. Similar to the ExoBrite™ CTB EV Stains, these Annexin V-based conjugates were developed for bright and sensitive staining of isolated EVs by flow cytometry with little to no background aggregation. ExoBrite™ Annexin EV Staining Kits also offer the broadest coverage for EVs. We tested EVs derived from 9 cell lines and ExoBrite™ Annexin EV Stains showed strong staining for all of them.

Advantages of ExoBrite™ Annexin EV Stains:

  • Optimally formulated Annexin V conjugates for staining purified EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Bright signal and low background staining for flow cytometry
  • Compatible with antibody co-staining
  • Available in 4 colors for flexible experimental design
ExoBrite™ Annexin EV Staining Kits
SEC-purified HeLa-derived EVs were stained with ExoBrite™ Annexin EV Stains.
ExoBrite™ 490/515 Annexin EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD9 in 100 uL, followed by 1X ExoBrite™ 655/670 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 655/670 WGA-positive particles, ~50% were also positive for CD9.

ExoBrite™ Annexin EV Staining Kits

Product Ex/Em Detection channels Size Catalog Number
ExoBrite™ 410/450
Annexin EV Staining Kit
416/452 nm Pacific Blue™ 100 Labelings 30119-T
500 Labelings 30119
ExoBrite™ 490/515
Annexin EV Staining Kit
490/516 nm FITC 100 Labelings 30120-T
500 Labelings 30120
ExoBrite™ 560/585
Annexin EV Staining Kit
562/584 nm PE 100 Labelings 30121-T
500 Labelings 30121
ExoBrite™ 655/670
Annexin EV Staining Kit
652/668 nm APC 100 Labelings 30122-T
500 Labelings 30122

EXOBRITE™ WGA EV STAINING KITS

Sensitive Stains for Purified or Bead-Bound EVs with Broad Compatibility

ExoBrite™ WGA EV Stains are fluorescent conjugates of wheat germ agglutinin (WGA) optimized for bright and sensitive staining of EVs. Similar to ExoBrite™ Annexin EV Stains, these WGA-based conjugates offer broad compatibility with EVs. The stains were validated for EVs derived from all 9 cell lines tested. In addition, ExoBrite™ WGA EV Stains are less prone to aggregation than hydrophobic membrane dyes and may be used to stain purified and bead-bound EVs.

Advantages of ExoBrite™ WGA EV Stains:

  • Optimally formulated WGA conjugates for staining purified or bead-bound EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Bright signal and low background staining for flow cytometry
  • Compatible with antibody co-staining
  • Available in 4 colors for flexible experimental design
ExoBrite™ WGA EV Staining Kits
SEC-purified EVs were stained with ExoBrite™ WGA EV Stains. MCF-7 derived EVs were stained with ExoBrite™ 410/450, ExoBrite™ 490/515, or ExoBrite™ 560/585. CHO derived EVs were stained with ExoBrite™ 640/660. 410/450 was detected in the Pacific Blue channel, 490/515 was detected in the FITC channel, 560/585 was detected in the PE channel, and 640/660 was detected in the APC channel.
ExoBrite™ 490/515 WGA EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified MCF-7-derived EVs were stained first with ExoBrite™ 490/515 CD81 in 100 uL, followed by 1X ExoBrite™ 640/660 WGA EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the FITC and APC channels. When we gated on ExoBrite™ 640/660 WGA-positive particles, ~60% were also positive for CD81.

ExoBrite™ WGA EV Staining Kits

Product Ex/Em Detection channels Size Catalog Number
ExoBrite™ 410/450
WGA EV Staining Kit
416/452 nm Pacific Blue™ 100 Labelings 30123-T
500 Labelings 30123
ExoBrite™ 490/515
WGA EV Staining Kit
490/516 nm FITC 100 Labelings 30124-T
500 Labelings 30124
ExoBrite™ 560/585
WGA EV Staining Kit
562/584 nm PE 100 Labelings 30125-T
500 Labelings 30125
ExoBrite™ 640/660
WGA EV Staining Kit
642/663 nm APC 100 Labelings 30126-T
500 Labelings 30126

EXOBRITE™ CTB EV STAINING KITS

Bright EV Stains with Excellent Signal-to-Noise

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection. ExoBrite™ stains bind to molecules in the EV membrane providing bright, specific staining of isolated EVs by flow cytometry. A key advantage of ExoBrite™ stains is that, unlike many other dyes, they show little to no background aggregates of a similar size as EVs. Also, unlike most membrane dyes, ExoBrite™ stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound EVs.

Advantages of ExoBrite™ CTB EV Stains:

  • Optimally formulated CTB conjugates for staining EVs
  • Designed for detection by flow cytometry
  • Bright fluorescence and low background for excellent signal-to-noise
  • Compatible with antibody co-staining
  • Stain purified or bead-bound EVs
  • Available in 4 colors for flexible experimental design
ExoBrite™ CTB EV Staining Kits
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ CTB EV Stains.
ExoBrite™ 490/515 CTB EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.
SEC-purified MCF-7-derived EVs were stained first with ExoBrite™ 490/515 CD9 in 100 uL, followed by 10X ExoBrite™ 560/585 CTB EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the FITC and R-PE channels. When we gated on ExoBrite™ 560/585 CTB-positive particles, ~95% were also positive for CD9.

ExoBrite™ CTB EV Staining Kits

Product Ex/Em Detection channels Size Catalog Number
ExoBrite™ 410/450
CTB EV Staining Kit
416/452 nm Pacific Blue™ 100 Labelings 30111-T
500 Labelings 30111
ExoBrite™ 490/515
CTB EV Staining Kit
490/516 nm FITC 100 Labelings 30112-T
500 Labelings 30112
ExoBrite™ 560/585
CTB EV Staining Kit
562/584 nm PE, Cy®3 100 Labelings 30113-T
500 Labelings 30113
ExoBrite™ 640/660
CTB EV Staining Kit
642/663 nm APC 100 Labelings 30114-T
500 Labelings 30114

CellMask is a trademark of Thermo Fisher Scientific; ExoFlow-ONE and ExoGlow are trademarks of System Biosciences.

ExoBrite™ Flow Antibody Conjugates

Validated antibodies for detection of EV markers by flow cytometry

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. ExoBrite™ Flow Antibody Conjugates were designed and validated for flow cytometry to offer bright signal and low background of EV markers in purified and bead-bound EVs.

ExoBrite™ Isotype Control Flow Antibody, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.

Advantages of ExoBrite™ Flow Antibodies:

  • Developed for detection of EV markers CD9, CD63, and CD81 by flow cytometry
  • Validated for purified or bead-bound EVs
  • Bright signal and low background
  • ExoBrite™ Isotype Control Flow Antibody available
  • Colors available for Pacific Blue™, FITC, and PE channels

Purified EVs stained with ExoBrite™ Flow Antibodies

SEC-purified A549-derived EVs were stained with ExoBrite™ 650/665 CD9 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the APC channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the PE channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD81 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Pacific Blue channel.
Product Conjugates Detection Channels Sizes Catalog Number
ExoBrite™ CD9 Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
ExoBrite™ 650/665
ExoBrite™ R-PE
Pacific Blue ™
FITC
PE
APC
25 tests
100 tests
P003-410… P003-RPE
ExoBrite™ CD9 (Mouse) Flow Antibody P018-410… P018-650
ExoBrite™ CD63 Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
ExoBrite™ R-PE
Pacific Blue ™
FITC
PE
25 tests
100 tests
P004-410… P004-RPE
ExoBrite™ CD63 (Mouse) Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
P022-410… P022-560
ExoBrite™ CD81 Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
ExoBrite™ R-PE
Pacific Blue ™
FITC
PE
25 tests
100 tests
P005-410… P005-RPE
ExoBrite™ CD81 (Mouse/Rat) Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
P019-410… P019-560
ExoBrite™ IgG1 Isotype Control Flow Antibody ExoBrite™ 410/450
ExoBrite™ 490/515
ExoBrite™ 560/585
ExoBrite™ 650/665
ExoBrite™ R-PE
Pacific Blue ™
FITC
PE
APC
25 tests
100 tests
P008-410… P008-RPE

ExoBrite™ Streptavidin Magnetic Beads

Convenient & Optimized Capture of EVs

Streamline your EV and exosome isolation workflows with ExoBrite™ Streptavidin Magnetic Beads, developed to offer lower background and higher sensitivity for EV capture than similar products. These streptavidin-coated and magnetic polystyrene beads (4.5 um diameter) may be used to isolate EVs from cell culture medium or other biological fluids without an overnight precipitation step.

ExoBrite™ Streptavidin Magnetic Beads

Advantages of ExoBrite™ Streptavidin Magnetic Beads:

  • Combine with a biotinylated antibody (not included) for isolation of EVs
  • Isolate EVs from biofluids without an overnight precipitation step
  • Lower background and higher sensitivity than competitors
  • Convenient magnetic-based separation
  • Detect downstream with microscopy, flow cytometry, or western blot
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD9 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD63 antibody, and then with concentrated MCF-7 cell supernatant. Bound EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.

 

ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CD9 Flow Antibody and were detected by flow cytometry in the PE channel.
ExoBrite™ Streptavidin Magnetic Beads were first incubated with (orange dots) or without (gray dots) biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. EVs were stained with ExoBrite™ 560/585 CTB EV Stain and were detected by flow cytometry in the PE channel.

SUPER-RESOLUTION IMAGING

See beyond the diffraction limit with
ExoBrite™ STORM CTB EV Staining Kits

Characterizing exosomes and EVs by imaging remains a challenge due to their small size and the resolution limit of light microscopy. Super-resolution microscopy techniques such as direct stochastic optical reconstruction microscopy (dSTORM) push beyond the diffraction limit of traditional light microscopy, allowing single-molecule resolution of subcellular structures such as EVs.

ExoBrite™ STORM CTB EV Stains were developed for STORM imaging of EVs and are available in four CF® Dyes validated for STORM.  ExoBrite™ 560/585 has been validated for dSTORM on the ONI Nanoimager S Mark II, allowing the study of fine morphological details and co-staining with EV biomarkers such as tetraspanin proteins CD9, CD63, and CD81.

Advantages of ExoBrite™ STORM CTB EV Stains:

  • Dive deeper into EV morphology and structure
  • Optimally formulated CTB conjugates for imaging EVs by STORM
  • Available in 4 CF® Dyes validated for STORM imaging
  • Allows antibody co-staining and localization studies with EV biomarkers
ExoBrite™ STORM CTB EV Staining Kits
Super-resolution dSTORM images of human colorectal cancer cell line derived EVs. EVs were stained with anti-tetraspanin antibodies (an anti-CD9/CD63/CD81 cocktail, all conjugated to CF®647, shown in magenta) together with 1X ExoBrite™ 560/585 (in cyan). Samples were prepared using ONI’s EV Profiler Kit and acquired using the Nanoimager S Mark II from ONI (Oxford Nanoimaging, UK). Data was processed using a beta-release version of CODI, ONI’s cloud-based data analysis platform. Scale bars are 500 nm (zoomed out, left panel) and 50 nm for single-EV panels (right). Image courtesy of ONI.
Product Ex/Em (nm) Laser Line(s) (nm) Detection Channel Size Catalog Number
ExoBrite™ STORM CF®505 CTB EV Staining Kit 505/519 488 FITC 100 Labelings 30115-T
500 Labelings 30115
ExoBrite™ STORM CF®583R CTB EV Staining Kit 583/609 555 or 561 Rhodamine or
Texas Red®
100 Labelings 30116-T
500 Labelings 30116
ExoBrite™ STORM CF®647 CTB EV Staining Kit 652/668 633-640 Cy®5 100 Labelings 30117-T
500 Labelings 30117
ExoBrite™ STORM CF®680 CTB EV Staining Kit 681/698 633-640 Cy®5.5 100 Labelings 30118-T
500 Labelings 30118

ExoBrite™ Western Antibody Conjugates

Validated antibodies for detection of EV markers by western blotting

ExoBrite™ Western Antibodies were validated to offer bright signal and low background of EV markers CD9, CD63, and CD81 in EV extracts by near-IR fluorescent western blot. The antibodies are available with two near-infrared fluorescent dyes, ExoBrite™ 680/700 and ExoBrite™ 770/800, which offer greater signal-to-noise than dyes with visible light emission for western blotting.

An ExoBrite™ Calnexin Western Antibody detects a protein of the endoplasmic reticulum that is not found in EVs. It is offered as a negative control to assess the purity of isolated EV extracts.

Advantages of ExoBrite™ Western Antibodies:

  • Developed for detection of EV markers CD9, CD63, and CD81 by fluorescent western blot
  • Validated for use with EV extracts
  • Bright signal and low background
  • Available in 2 near-infrared colors
  • Negative control ExoBrite™ Calnexin Western Antibody available
Western detection of human CD9 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD9 Western Antibody, showing enrichment of CD9 in the EV prep.
Western detection of human CD63 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD63 Western Antibody, showing enrichment of CD63 in the EV prep.
Western detection of human CD81 in MCF-7 cell and EV lysate using ExoBrite™ 680/700 CD81 Western Antibody, showing enrichment of CD81 in the EV prep.
Western detection of human calnexin in MCF-7 cell and EV lysate using ExoBrite™ 770/800 calnexin Western Antibody, showing the lack of calnexin in the EV prep.
Product Conjugates Sizes Catalog Number
ExoBrite™ CD9 Western Antibody ExoBrite™ 680/700
ExoBrite™ 770/800
25 tests
100 tests
P003-680, P003-770
ExoBrite™ CD63 Western Antibody P004-680, P004-770
ExoBrite™ CD81 Western Antibody P006-680, P006-770
ExoBrite™ Calnexin Western Antibody ExoBrite™ 770/800 P007-770

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TOTAL RNA EXTRACTION

Efficient total RNA extraction from purified EVs

The ExoBrite™ EV Total RNA Isolation Kit was designed to address the challenges of EV RNA extraction by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNAs, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.

Advantages of ExoBrite™ EV Total RNA Isolation Kit:

  • Optimized for total RNA extraction from purified EVs
  • Recover ~10-20 ng of RNA from 1×1010 SEC-enriched EVs
  • Compatible with downstream applications such as qPCR or RNAseq
  • Simple column-based purification
  • No phenol/chloroform or ethanol precipitation steps