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PROTEIN GEL STAINS

One-Step Blue®, One-Step Lumitein™ & One-Step Lumitein™ UV

One-Step Protein Gel Stains
Low-cost, rapid, non-toxic protein gel stains
A safer and more convenient alternative to tedious Coomassie blue staining
Replacements for costly and time consuming SYPRO Ruby and Oriole stains

Our One-Step protein gel stains are low-cost, quick, convenient and easy-to-use for protein gel (SDS-PAGE) staining. Staining is quick: 5 to 60 min in a single step without fixation or washing. These stains have non-toxic formulations for safer handling and easy disposal. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. For more information download the Safety Report for One-Step Protein Gel Stains.

The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry.

  • 100% H2O-based formulation
  • NO stinky acetic acid
  • NO toxic methanol
  • NO microwaving
  • NO washing
  • NO fixing

One-Step Blue® Protein Gel Stain

One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.

  • One-step staining procedure, no fixation required
  • Non-toxic formulation is safe for easy disposal
  • Sensitive protein detection down to 10-20 ng
  • Visualize blue bands, or image with a near-infrared fluorescence imager

Fast and simple procedure

One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In a comparison experiment with Coomassie Brilliant Blue R-250 and Simply Blue™ SafeStain, One-Step Blue® demonstrated much clearer staining after 1 hour of destaining in water compared to other stains (Figure 2.).

Figure 1. One-Step Blue® is an alternative to tedious Coomassie staining, and can be imaged visually or using a near-infrared fluorescence imager.
Figure 2. Unstained protein ladder was loaded on a 4-12% Bis-Tris gel and stained for 1 hour with Coomassie Brilliant Blue R-250 (CBB), Simply Blue™ SafeStain (SB), and One-Step Blue® (OSB) following SDS-PAGE. Gels stained with Simply Blue™ SafeStain, and One-Step Blue® were destained in water for 1 hour. The gel stained with CBB was destained in 5% MeOH, and 7.5% acetic acid for 1 hour.

One-Step Lumitein™ & One-Step Lumitein™ UV

One-Step Lumitein™ and One-Step Lumitein™ UV are ready-to-use luminescent red protein gel stains that feature a convenient one-step fixation procedure with excellent sensitivity. Both gel stains are non-toxic and offer sensitive detection down to 1-10 ng in as little as 5 minutes. Staining is fully compatible with mass spectrometry and Edman-based sequencing.

One-Step Lumitein™ serves as an alternative to SYPRO® Ruby and may be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.

One-Step Lumitein™ UV is optimized for use on UV transilluminators and serves as a replacement for Oriole™ and Flamingo™ Protein Gel Stains. In addition, One-Step Lumitein™ UV is an aqueous-based solution that does not contain hazardous solvents that cause gel shrinkage, unlike Oriole™ Fluorescent Gel Stain (Figure 4.)

  • One-step staining procedure, no fixation required
  • Non-toxic formulation is safe for easy disposal
  • Sensitive protein detection down to 1-10 ng
  • One-Step Lumitein™ as a replacement for SYPRO® Ruby
  • One-Step Lumitein™ UV as a replacement for Oriole™ and Flamingo™ Protein Gel Stains

Figure 3. Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or laser gel scanner
Figure 4. One-Step Lumitein™ UV Gel Stain is an alternative to Oriole™ and Flamingo™ Gel Stains. Best stain for UV transilluminator. It does not contain hazardous solvents or shrink gels.

Ordering Information

ProductReplacement forCatalog numberUnit sizeProduct protocolSafety report
One-Step Blue®
Coomassie stain21003-1L1LPI-21003
One-Step Gel Stains Safety Report
One-Step Lumitein™SYPRO® Ruby Protein Gel Stain21004-1L1LPI-21004
21004-4L4L
One-Step Lumitein™ UVOriole™ and Flamingo™ Protein Gel Stains21005-1L1LPI-21005
21005-4L4L

One-Step Blue is a registered trademark of Biotium.

Lumitein and its related technologies are covered by US and international patents.
SYPRO is a registered trademark of Molecular Probes, Inc.
Oriole and Flamingo are trademarks of Bio-Rad Laboratories.
SimplyBlue is a trademark of Thermo Fisher Scientific.

One-Step Blue® Protein Gel Stain

From: $92 Sizes: 1 LCatalog #: 21003-1L

One-Step Lumitein™ Protein Gel Stain

From: $118 Sizes: 1 L, 4 LCatalog #: 21004-1L, - 21004-4LView allHide

One-Step Lumitein™ UV Protein Gel Stain

From: $160 Sizes: 1 L, 4 LCatalog #: 21005-1L, - 21005-4LView allHide