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Viability PCR Reagents

Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal PCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantified viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses and parasites.

As the inventor of both PMAxx™ and PMA v-PCR dyes, Biotium offers expertise, and the widest selection of v-PCR dyes, kits and accessory products.

To learn more about viability PCR, visit our technology page.

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    Glo-Plate™ Blue LED Illuminator

    Sizes: EachCatalog #: E90004

    The Glo-Plate™ Blue LED Illuminator is a multi-functional blue LED light box. It can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure®.

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    The Glo-Plate™ Blue LED Illuminator is a multi-functional blue LED light box. It can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure®.

     

    PMA Dye

    From: $132 Sizes: 1 mgCatalog #: 40013

    PMA (propidium monoazide) is a high affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other microorganisms.

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    PMA (propidium monoazide) is a high affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other microorganisms.

     

    PMA Dye, 20 mM in H2O

    From: $127 Sizes: 100 uLCatalog #: 40019

    PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

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    PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $74 Sizes: 16 mLCatalog #: 31038

    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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    16 mL
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    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $315 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $315 Sizes: 200 assaysCatalog #: 31037, - 31037-XView allHide

    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $315 Sizes: 200 assaysCatalog #: 31053

    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $315 Sizes: 200 assaysCatalog #: 31051, - 31051-XView allHide

    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $315 Sizes: 200 assaysCatalog #: 31034

    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $315 Sizes: 200 assaysCatalog #: 31033, - 31033-XView allHide

    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $315 Sizes: 200 assaysCatalog #: 31036

    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $315 Sizes: 200 assaysCatalog #: 31035

    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA-Lite™ LED Photolysis Device

    From: $1,943 Sizes: EachCatalog #: E90002

    PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

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    PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

     

    PMAxx™, 20 mM in H2O

    From: $127 Sizes: 100 uLCatalog #: 40069

    PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide).

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    PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide).

     

    Viability PCR Starter Kits

    From: $290 Sizes: 200 assaysCatalog #: 31075, 31075-X, 31076, - 31076-XView allHide

    Viability PCR starter kits contain the materials that you need for selective detection of viable cells using either PMA or PMAxx™ viability dye and real-time PCR. All of the kits contain Forget-Me-Not™ qPCR Master Mix, and your choice of PMA or PMAxx™ dye.

    Viability dye
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    200 assays
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    Viability PCR starter kits contain the materials that you need for selective detection of viable cells using either PMA or PMAxx™ viability dye and real-time PCR. All of the kits contain Forget-Me-Not™ qPCR Master Mix, and your choice of PMA or PMAxx™ dye.

     

    Ethidium Monoazide, Bromide (EMA)

    From: $151 Sizes: 5 mgCatalog #: 40015

    Ethidium monoazide bromide is a fluorescent nucleic acid binding dye. The dye, after photolysis, binds covalently to nucleic acids. It can be used for viability PCR.

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    Ethidium monoazide bromide is a fluorescent nucleic acid binding dye. The dye, after photolysis, binds covalently to nucleic acids. It can be used for viability PCR.