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Viability PCR Reagents

Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal PCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantified viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses and parasites.

As the inventor of both PMAxx™ and PMA v-PCR dyes, Biotium offers expertise, and the widest selection of v-PCR dyes, kits and accessory products.

To learn more about viability PCR, visit our technology page.

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    Product

    PMAxx™ Dye, 20 mM in H2O

    From: $143 Sizes: 100 uLCatalog #: 40069

    Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).

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    100 uL
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    Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).

     
    Product

    PMA (Propidium Monoazide)

    From: $143 Sizes: 100 uL, 1 mgCatalog #:
    40013
    , - 40019View allHide

    A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.

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    100 uL
    1 mg
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    A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.

     
    Product

    Viability PCR Starter Kits

    From: $318 Sizes: 200 assaysCatalog #:
    31075
    , 31075-X, 31076, - 31076-XView allHide

    Kits designed to make it simple to start performing viability PCR with your choice of viability dye (PMA or PMAxx™).

    Viability dye
    Bacteria type
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    200 assays
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    Kits designed to make it simple to start performing viability PCR with your choice of viability dye (PMA or PMAxx™).

     
    Product

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $83 Sizes: 16 mLCatalog #: 31038

    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

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    16 mL
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    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

     
    Product

    PMA-Lite™ LED Photolysis Device

    From: $2,197 Sizes: EachCatalog #: E90002

    A blue LED photolysis device designed for photoactivation of PMAxx™ and PMA in viability PCR.

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    Each
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    A blue LED photolysis device designed for photoactivation of PMAxx™ and PMA in viability PCR.

     
    Product

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $385 Sizes: 200 assaysCatalog #:
    31050
    , - 31050-XView allHide

    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

     
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    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $385 Sizes: 200 assaysCatalog #:
    31037
    , - 31037-XView allHide

    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

    Viability dye
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    Qty
    200 assays
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    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

     
    Product

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $385 Sizes: 200 assaysCatalog #: 31053

    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

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    200 assays
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    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

     
    Product

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $355 Sizes: 200 assaysCatalog #:
    31051
    , - 31051-XView allHide

    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

    Viability dye
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    Qty
    200 assays
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    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

     
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    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $355 Sizes: 200 assaysCatalog #: 31034

    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

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    Qty
    200 assays
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    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

     
    Product

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $385 Sizes: 200 assaysCatalog #:
    31033
    , - 31033-XView allHide

    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

    Viability dye
    Size
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    Qty
    200 assays
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    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

     
    Product

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $355 Sizes: 200 assaysCatalog #: 31035

    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

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    200 assays
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    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

     
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    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $355 Sizes: 200 assaysCatalog #: 31036

    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

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    200 assays
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    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

     
    Product

    Ethidium Monoazide Bromide (EMA)

    From: $180 Sizes: 5 mgCatalog #: 40015

    A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.

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    5 mg
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    A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.