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Viability PCR Reagents

Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal PCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantified viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses and parasites.

As the inventor of both PMAxx™ and PMA v-PCR dyes, Biotium offers expertise, and the widest selection of v-PCR dyes, kits and accessory products.

To learn more about viability PCR, visit our technology page.

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    Glo-Plate™ Blue LED Illuminator

    Sizes: EachCatalog #: E90004

    A multi-functional blue LED light box that can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure®.

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    A multi-functional blue LED light box that can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure®.

     

    PMA (Propidium Monoazide)

    From: $136 Sizes: 1 mgCatalog #: 40013

    PMA (propidium monoazide) is a photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other microorganisms.

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    PMA (propidium monoazide) is a photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other microorganisms.

     

    PMA Dye, 20 mM in H2O

    From: $131 Sizes: 100 uLCatalog #: 40019

    PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

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    PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $76 Sizes: 16 mLCatalog #: 31038

    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

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    16 mL
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    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $350 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

    Viability dye
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    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $350 Sizes: 200 assaysCatalog #: 31037, - 31037-XView allHide

    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $350 Sizes: 200 assaysCatalog #: 31053

    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

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    200 assays
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    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $324 Sizes: 200 assaysCatalog #: 31051, - 31051-XView allHide

    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $324 Sizes: 200 assaysCatalog #: 31034

    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

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    200 assays
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    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $350 Sizes: 200 assaysCatalog #: 31033, - 31033-XView allHide

    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $324 Sizes: 200 assaysCatalog #: 31036

    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

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    200 assays
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    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $324 Sizes: 200 assaysCatalog #: 31035

    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

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    200 assays
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    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

     

    PMA-Lite™ LED Photolysis Device

    From: $2,001 Sizes: EachCatalog #: E90002

    PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

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    PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

     

    PMAxx™ Dye, 20 mM in H2O

    From: $131 Sizes: 100 uLCatalog #: 40069

    PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide).

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    PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide).

     

    Viability PCR Starter Kits

    Sizes: 200 assaysCatalog #: 31075, 31075-X, 31076, - 31076-XView allHide

    Viability PCR starter kits contain the materials that you need for selective detection of viable cells using either PMA or PMAxx™ viability dye and real-time PCR. All of the kits contain Forget-Me-Not™ qPCR Master Mix, and your choice of PMA or PMAxx™ dye.

    Viability dye
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    200 assays
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    Viability PCR starter kits contain the materials that you need for selective detection of viable cells using either PMA or PMAxx™ viability dye and real-time PCR. All of the kits contain Forget-Me-Not™ qPCR Master Mix, and your choice of PMA or PMAxx™ dye.

     

    Ethidium Monoazide Bromide (EMA)

    Sizes: 5 mgCatalog #: 40015

    A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.

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    5 mg
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    A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.