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NUCVIEW® CASPASE-3 SUBSTRATES

The Only Caspase Substrates for Real-Time Apoptosis Detection

Real-time apoptosis detection
Substrate doesn't interfere with apoptosis progression.
For flow cytometry, microscopy, or live cell imaging.
Fast, simple, and fixable
Homogenous, no wash 15-30 minute assay.
Formaldehyde-fixable nuclear staining.
Three color options
Blue fluorescent NucView® 405
Green fluorescent NucView® 488
Orange fluorescent NucView® 530

NucView® caspase substrates

NucView® Caspase-3 Substrates are novel fluorogenic substrates developed by Biotium to detect apoptosis in intact cells in real-time. Biotium has invented a new concept in the design of fluorogenic enzyme substrates. The enzyme substrate is attached to a fluorogenic DNA dye. Before cleavage, the dye is non-fluorescent and unable to bind to DNA. Upon enzymatic cleavage of the substrate, the dye is released and becomes capable of binding to DNA to emit fluorescence. In the case of NucView® Caspase-3 Substrates, the caspase-3 substrate peptide DEVD is attached to a DNA-binding dye. The substrate enters the cell cytoplasm where it is cleaved by caspase-3 in apoptotic cells to release the fluorogenic DNA dye, which stains the nucleus. Unlike FLICA assays that use irreversible inhibitors to label active caspases, NucView® Caspase-3 Substrates do not interfere with caspase activity, allowing monitoring of caspase activity in real time.

Features

  • Simple, homogenous assay for endpoint assay or real-time detection in live cells
  • Non-toxic, allowing for multi-day experiments
  • Detect caspase-3 activity and visualize apopototic nuclear morphology
  • For flow cytometry, fluorescence microscopy, or live cell imaging systems
  • Available as stand-alone substrates or in kits with other apoptosis or necrosis probes
  • Available with blue, green, or red fluorescence
  • NucView® 488 validated in more than 100 cell types and 200 publications – See the list

Principle of NucView® substrate technology. The substrate is non-fluorescent and does not bind DNA. After enzyme cleavage, the high-affinity DNA dye is released to bind DNA and become fluorescent.

NucView® 488 Caspase-3 Enzyme Substrate in action. HT-1080 cells were treated with camptothecin and NucView® 488 Caspase-3 Substrate and imaged every 30 minutes for 24 hours on the IncuCyte® Zoom. Apoptotic cell nuclei fluoresce green as NucView® 488 dye is released by caspase-3 cleavage. Video courtesy of Essen Biosciences.

Apoptotic Jurkat cell stained with NucView® 488 (green) and CF®647 Annexin V (magenta).

Time course of caspase-3 detection with NucView® 405 Caspase-3 Substrate in Jurkat cells treated with staurosporine to induce apoptosis, analyzed in the Pacific Blue® channel of a BD LSRII flow cytometer (405 nm excitation, 450/50 nm emission filter).


MCF-7 cells were left untreated or treated with staurosporine overnight to induce apoptosis, then stained for 30 minutes at 37C with 40 nM MitoView™ Blue, 2 uM NucView® 530 Caspase-3 Substrate, and 100 ng/mL CF®488A Annexin V in cell culture medium. Healthy cells show mitochondrial staining with MitoView™ Blue (cyan), while apoptotic cells show NucView® 530 staining of nuclei (red) and CF®488A Annexin V staining of cell membranes (green).

ProductCatalog No.Features
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO10405Blue fluorescence for flow cytometry in the Pacific Blue® channel or microscopy with the 405 nm laser
NucView® 405 Caspase-3 Substrate, 1 mM in PBS10407NucView® 405 substrate in PBS, for DMSO-sensitive cell types
NucView® 488 Caspase-3 Substrate, 1 mM in DMSO10402Green fluorescent substrate validated in more than 100 cell types and 200 publications
NucView® 488 Caspase-3 Substrate, 1 mM in PBS10403NucView® 488 substrate in PBS, for DMSO-sensitive cell types
NucView® 530 Caspase-3 Substrate, 1 mM in DMSO10406Orange fluorescence for microscopy in the Cy®3 channel or flow cytometry in the R-PE channel
NucView® 530 Caspase-3 Substrate, 1 mM in PBS10408NucView® 530 substrate in PBS, for DMSO-sensitive cell types
NucView® 488 and MitoView™ 633 Apotosis Detection Kit30062NucView® 488 and far-red fluorescent MitoView™ 633 for the Cy®5 channel
NucView® 488 and RedDot™ 2 Apoptosis & Necrosis Kit30072NucView® 488 and far-red dead cell DNA dye RedDot™ 2 for the Cy®5 channel
Dual Apoptosis Assay with NucView® 488 and CF®594 Annexin V30067NucView® 488 and red fluorescent Annexin V apoptosis probe
Dual Apoptosis Assay with NucView® 488 and CF®640R Annexin V30073NucView® 488 and far-red fluorescent Annexin V apoptosis probe

Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

From: $341 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

NucView® 405 Caspase-3 Substrate, 1 mM in DMSO

From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10405, - 10405-TView allHide

NucView® 488 and MitoView™ 633 Apoptosis Assay Kit

From: $341 Sizes: 100 assaysCatalog #: 30062

NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit

From: $299 Sizes: 100 assaysCatalog #: 30072

NucView® 488 Caspase-3 Assay Kit for Live Cells

From: $92 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in DMSO

From: $63 Sizes: 10 uL, 100 uLCatalog #: 10402, - 10402-TView allHide

NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in PBS

From: $63 Sizes: 10 uL, 100 uLCatalog #: 10403, - 10403-TView allHide

NucView® 530 Caspase-3 Substrate, 1 mM in DMSO

From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10406, - 10406-TView allHide

 

Faq

NucView® Caspase 3 Enzyme Substrates

The substrates are very stable. Some users have reported performing time course assays with NucView® 488 Caspase-3 Substrate for 4-5 days.

Category: NucView® Caspase 3 Enzyme Substrates

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NucView® Caspase-3 Substrates can be added to the cells at the start of the experiment or at the end. A major advantage of NucView® Caspase-3 Substrates compared to other apoptosis assays is that it can be used to monitor capase-3 activity in real time.

Category: NucView® Caspase 3 Enzyme Substrates

← FAQs
Yes. NucView® Caspase-3 Substrates are compatible with flow cytometers and other instruments with the appropriate excitation and emission settings.

Category: NucView® Caspase 3 Enzyme Substrates

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