For flow cytometry, microscopy, or live cell imaging
Formaldehyde-fixable nuclear staining
Green fluorescent NucView 488
Orange fluorescent NucView 530
NucView™ 488 Caspase-3 Enzyme Substrate in action. HT-1080 cells were treated with camptothecin and NucView™ 488 Caspase-3 Substrate and imaged every 30 minutes for 24 hours on the IncuCyte® Zoom. Apoptotic cell nuclei fluoresce green as NucView™ 488 dye is released by caspase-3 cleavage. Video courtesy of Essen Biosciences.
NucView™ Caspase-3 Substrates are available as a stand-alone substrates, or in kits with other fluorescent apoptosis and necrosis probes for studying the temporal and spatial relations among the different events by fluorescence microscopy or flow cytometry. See NucView™ products…
NucView™ Caspase 3 Enzyme Substrates
Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.
One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.
The substrate is very stable. Some users have reported performing time course assays with NucView™ 488 Caspase-3 Substrate for 4-5 days.← FAQs
NucView™ Caspase-3 Substrates can be added to the cells at the start of the experiment or at the end. A major advantage of NucView™ Caspase-3 Substrates compared to other apoptosis assays is that it can be used to monitor capase-3 activity in real time.← FAQs