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Cytosolic Tracers

Biotium offers an array of fluorescent cytosolic tracers for various cell types and applications:

ViaFluor® SE Cell Proliferation Dyes are non-toxic and can be used to stain cells for long-term, fixable cell tracing for flow or microscopy. CFDA and Calcein AM are membrane-permeant esterase substrates with green fluorescence. CF® Dye-labeled molecules such as Dextran and BSA can be used to observe endocytosis. Polar tracers used to trace neurons and cell junctions include CF® Dye-labeled Biocytin, Hydrazide,  and Fluoro-Gold™.

See our full selection of cytosolic tracers on our Cellular Stains page.

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    ViaFluor® SE Cell Proliferation Kits

    From: $50 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #: 30050, 30068, 30086, 30068-T, - 30086-TView allHide

    Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

    Dye Color
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

     

    CF® Dye Hydrazide

    From: $168 Sizes: 1 mgCatalog #: 96063, 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 96024, - 96025View allHide

    Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

     

    Bovine Serum Albumin CF® Dye Conjugates

    Sizes: 5 x 1 mgCatalog #: 20289, 20290, 20291, - 20292View allHide

    Bright and photostable fluorescent BSA conjugates for applications in which well-defined molecular weight fluid phase tracers are needed. These applications include studying the rate of endocytosis and exocytosis and following vesicle trafficking.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Bright and photostable fluorescent BSA conjugates for applications in which well-defined molecular weight fluid phase tracers are needed. These applications include studying the rate of endocytosis and exocytosis and following vesicle trafficking.

     

    CF® Dye Dextran 10,000 MW, Anionic and Fixable

    From: $90 Sizes: 1 mgCatalog #: 80110, 80111, 80112, 80113, 80114, 80115, 80116, 80118, 80119, 80120, - 80121View allHide

    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10,000 MW, and contains a formaldehyde-fixable free-amine group.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10,000 MW, and contains a formaldehyde-fixable free-amine group.

     

    CF® Dye Dextran 150,000 MW, Anionic and Fixable

    From: $96 Sizes: 1 mgCatalog #: 80124, 80131, 80132, - 80133View allHide

    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

     

    CF® Dye Dextran 250,000 MW, Anionic and Fixable

    From: $96 Sizes: 1 mgCatalog #: 80125, 80134, 80135, - 80136View allHide

    This dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    This dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

     

    CF® Dye Dextran 3,500 MW, Anionic and Fixable

    From: $101 Sizes: 1 mgCatalog #: 80137

    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

     

    CF® Dye Dextran 40,000 MW, Anionic and Fixable

    From: $96 Sizes: 1 mgCatalog #: 80122, 80126, 80127, - 80128View allHide

    This dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    This dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

     

    CF® Dye Dextran 70,000 MW, Anionic and Fixable

    From: $96 Sizes: 1 mgCatalog #: 80117, 80123, 80129, - 80130View allHide

    This dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    This dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.

     

    CF®DI Hydrazide

    From: $168 Sizes: 1 mgCatalog #: 92164, 92165, 92166, 92184, - 92185View allHide

    CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

     

    5-(and 6)-Carboxy-2′,7′- dichlorofluorescein diacetate

    Sizes: 100 mgCatalog #: 51016

    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mg
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    5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

     

    5-(and 6)-CFDA

    Sizes: 100 mgCatalog #: 51014

    5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mg
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    5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).

     

    5-(and-6)-Carboxy-2′,7′- dichlorofluorescein

    Sizes: 200 mgCatalog #: 51015

    5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    200 mg
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    5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.

     

    5-(and-6)-Carboxyfluorescein

    Sizes: 100 mgCatalog #: 51013

    5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.

     

    5-Carboxy-2′,7′-dichlorofluorescein (single isomer)

    Sizes: 100 mgCatalog #: 51017

    This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mg
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    This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.

     

    5-Carboxy-2′,7′-dichlorosulfonefluorescein

    Sizes: 10 mgCatalog #: 51023

    The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 mg
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    The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.