Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

DNA/RNA Dyes & Nuclear Stains

For nuclear staining of live cells, we offer NucSpot® Live stains available in green or far-red, and RedDot™1, a far-red nuclear stain.

For nuclear staining of fixed cells, see NucSpot® 470, a green nuclear stain, and RedDot™2, a far-red nuclear dye.

Biotium offers a series of asymmetric cyanine dyes, equivalent to SYBR® Green and others: Thiazole Green (aka SYBR® Green I), Thiazole Orange, Thiazole Orange Homodimer (aka TOTO®-1), Thiazole Orange Iodide (aka TO-PRO®-1), Oxazole Yellow (aka YO-PRO®-1), Thiazole Red (aka TO-PRO®-3), and Thiazole Red Homodimer (aka TOTO®-3).

Ethidium Homodimer III (EthD-III) is a far-red dead-cell stain, also sold together with a live cell stain.

We also offer classical nuclear stains such as DAPI, Hoechst, Propidium Iodide (PI), 7-AAD, and Acridine Orange (AO).

REFINE BY

Showing 1–16 of 31 results

    NucSpot® Live Cell Nuclear Stains

    Sizes: Trial size (10 uL), 50 uLCatalog #: 40082-T, 40082, 40081-T, - 40081View allHide

    NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have have low toxicity for live cell imaging. Supplied as 1000X stock solutions in DMSO.

    Dye
    Size
    Catalog #
    price
    Qty
    Trial size (10 uL)
    50 uL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have have low toxicity for live cell imaging. Supplied as 1000X stock solutions in DMSO.

     

    NucSpot® 470 Nuclear Stain, 1000X in DMSO

    Sizes: 20 uL, 200 uLCatalog #: 40083-T, - 40083View allHide

    NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.

    Size
    Catalog #
    price
    Qty
    20 uL
    200 uL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.

     

    RedDot™1 Far-Red Nuclear Stain, 200X in H2O

    From: $47 Sizes: 25 uL (15-20 tests, trial size), 250 uL (150-200 tests), 1 mL (600-1000 tests)Catalog #: 40060, 40060-1, - 40060-TView allHide

    RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

    Size
    Catalog #
    price
    Qty
    25 uL (15-20 tests, trial size)
    250 uL (150-200 tests)
    1 mL (600-1000 tests)
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

     

    RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

    From: $68 Sizes: 25 uL (15-20 tests, trial size), 250 uL (150-200 tests), 1 mL (600-1000 tests)Catalog #: 40061, 40061-1, - 40061-TView allHide

    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

    Size
    Catalog #
    price
    Qty
    25 uL (15-20 tests, trial size)
    250 uL (150-200 tests)
    1 mL (600-1000 tests)
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

     

    DMAO, 2 mM in DMSO

    From: $221 Sizes: 1 mLCatalog #: 40012

    DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.

    Size
    Catalog #
    price
    Qty
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.

     

    Ethidium Homodimer I (EthD-1; EtDi)

    From: $173 Sizes: 1 mgCatalog #: 40010

    Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain.

     

    Ethidium Homodimer III (EthD-III)

    From: $242 Sizes: 1 mgCatalog #: 40050

    Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.

     

    Ethidium Homodimer III (EthD-III), 1 mM in DMSO

    From: $236 Sizes: 200 uLCatalog #: 40051

    Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.

    Size
    Catalog #
    price
    Qty
    200 uL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.

     

    Oxazole Yellow, 1mM in DMSO

    Sizes: 1 mLCatalog #: 40089

    Oxazole yellow, also know as YO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 491/509 nm when bound to nucleic acids. Oxazole yellow is also used to identify apoptotic cells. Supplied at 1 mM in DMSO.

    Size
    Catalog #
    price
    Qty
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Oxazole yellow, also know as YO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 491/509 nm when bound to nucleic acids. Oxazole yellow is also used to identify apoptotic cells. Supplied at 1 mM in DMSO.

     

    Thiazole Green, 10,000X in DMSO

    Sizes: 0.5 mL, 1 mLCatalog #: 40086-0.5mL, - 40086-1mLView allHide

    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

    Size
    Catalog #
    price
    Qty
    0.5 mL
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

     

    Thiazole Orange Homodimer, 1 mM in DMSO

    Sizes: 200 uLCatalog #: 40079

    Thiazole orange homodimer (TOhD), also know as TOTO-1, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~514/533 nm when bound to nucleic acids.

    Size
    Catalog #
    price
    Qty
    200 uL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Thiazole orange homodimer (TOhD), also know as TOTO-1, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~514/533 nm when bound to nucleic acids.

     

    Thiazole Orange, 10 mM in DMSO

    Sizes: 1 mLCatalog #: 40077

    Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.

    Size
    Catalog #
    price
    Qty
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.

     

    Thiazole Red Homodimer, 1mM in DMSO

    Sizes: 200 uLCatalog #: 40080

    Thiazole red homodimer (TRhD), also know as TOTO-3, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~642/660 nm when bound to nucleic acids.

    Size
    Catalog #
    price
    Qty
    200 uL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Thiazole red homodimer (TRhD), also know as TOTO-3, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~642/660 nm when bound to nucleic acids.

     

    Thiazole Red, 1mM in DMSO

    Sizes: 1 mLCatalog #: 40087

    Thiazole red, also known as TO-PRO-3, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima ~642/661 nm when bound to nucleic acids.

    Size
    Catalog #
    price
    Qty
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    Thiazole red, also known as TO-PRO-3, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima ~642/661 nm when bound to nucleic acids.

     

    TO iodide (515/531), 1 mM in DMSO

    Sizes: 1 mLCatalog #: 40088

    TO iodide, also know as TO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 515/531 nm when bound to nucleic acids.

    Size
    Catalog #
    price
    Qty
    1 mL
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    TO iodide, also know as TO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 515/531 nm when bound to nucleic acids.

     

    7-AAD

    From: $110 Sizes: 1 mgCatalog #: 40037

    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.

    Size
    Catalog #
    price
    Qty
    1 mg
    Clear selection
    Add to wishlist
    ADD TO CART
    (Please select quantity above )

    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.