DNA/RNA Dyes & Nuclear Stains
For nuclear staining of live cells, we offer NucSpot® Live stains available in green or far-red, and RedDot™1, a far-red nuclear stain.
For nuclear staining of fixed cells, see NucSpot® 470, a green nuclear stain, and RedDot™2, a far-red nuclear dye.
Biotium offers a series of asymmetric cyanine dyes, equivalent to SYBR® Green and others: Thiazole Green (aka SYBR® Green I), Thiazole Orange, Thiazole Orange Homodimer (aka TOTO®-1), Thiazole Orange Iodide (aka TO-PRO®-1), Oxazole Yellow (aka YO-PRO®-1), Thiazole Red (aka TO-PRO®-3), and Thiazole Red Homodimer (aka TOTO®-3).
Ethidium Homodimer III (EthD-III) is a far-red dead-cell stain, also sold together with a live cell stain.
We also offer classical nuclear stains such as DAPI, Hoechst, Propidium Iodide (PI), 7-AAD, and Acridine Orange (AO).
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NucSpot® Live Cell Nuclear Stains
NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have have low toxicity for live cell imaging. Supplied as 1000X stock solutions in DMSO.
NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have have low toxicity for live cell imaging. Supplied as 1000X stock solutions in DMSO.
NucSpot® 470 Nuclear Stain, 1000X in DMSO
NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.
NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.
RedDot™1 Far-Red Nuclear Stain, 200X in H2O
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
RedDot™2 Far-Red Nuclear Stain, 200X in DMSO
RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.
RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.
DMAO, 2 mM in DMSO
DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.
DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.
Ethidium Homodimer I (EthD-1; EtDi)
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain.
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain.
Ethidium Homodimer III (EthD-III)
Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.
Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.
Ethidium Homodimer III (EthD-III), 1 mM in DMSO
Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.
Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.
Oxazole Yellow, 1mM in DMSO
Oxazole yellow, also know as YO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 491/509 nm when bound to nucleic acids. Oxazole yellow is also used to identify apoptotic cells. Supplied at 1 mM in DMSO.
Oxazole yellow, also know as YO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 491/509 nm when bound to nucleic acids. Oxazole yellow is also used to identify apoptotic cells. Supplied at 1 mM in DMSO.
Thiazole Green, 10,000X in DMSO
Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
Thiazole Orange Homodimer, 1 mM in DMSO
Thiazole orange homodimer (TOhD), also know as TOTO-1, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~514/533 nm when bound to nucleic acids.
Thiazole orange homodimer (TOhD), also know as TOTO-1, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~514/533 nm when bound to nucleic acids.
Thiazole Orange, 10 mM in DMSO
Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.
Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.
Thiazole Red Homodimer, 1mM in DMSO
Thiazole red homodimer (TRhD), also know as TOTO-3, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~642/660 nm when bound to nucleic acids.
Thiazole red homodimer (TRhD), also know as TOTO-3, is a cell-impermeant, high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~642/660 nm when bound to nucleic acids.
Thiazole Red, 1mM in DMSO
Thiazole red, also known as TO-PRO-3, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima ~642/661 nm when bound to nucleic acids.
Thiazole red, also known as TO-PRO-3, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima ~642/661 nm when bound to nucleic acids.
TO iodide (515/531), 1 mM in DMSO
TO iodide, also know as TO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 515/531 nm when bound to nucleic acids.
TO iodide, also know as TO-PRO-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 515/531 nm when bound to nucleic acids.
7-AAD
7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.
7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.