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Primary Antibody Conjugates

Featuring validated Biotium Choice antibodies with CF® Dyes

Curated primary antibodies developed for optimal performance in flow cytometry and other applications
Growing collection of over 2000 monoclonal antibodies with label options for 6 CF® Dyes or biotin
Choose from validated antibodies labeled with bright
CF® Dyes, Astral™ tandem dyes, and other labels

BIOTIUM CHOICE ANTIBODIES – CURATED, VALIDATED, OPTIMIZED

Be Confident in Your Results with Biotium Choice

Our line of Biotium Choice monoclonal antibodies are carefully curated and extensively validated in-house for optimal performance in flow cytometry and other applications. The antibodies are available in a selection of CF® Dyes and other probes, with new antibody clones and conjugates on the way.

Biotium Choice Antibody Features

  • Widely published clones against common targets
  • Developed and optimized for flow cytometry and other applications
  • Available with bright CF® Dyes, Astral™ tandem dyes, and more
  • New antibody clones and conjugate options on the way

Identify a Biotium Choice Antibody

Look for this symbol on product pages and search results

Methanol-fixed MCF-7 cells were stained with CD9 Mouse Monoclonal Antibody (HI9a) followed by goat anti-mouse CF®568. The nuclei were stained with DAPI, and the cells were imaged in the Cy®3 and DAPI channels on a Zeiss LSM 700 confocal microscope using a 40X oil objective.
Flow cytometry of lymphocyte-gated PBMCs unstained (gray) or stained with CD4 Mouse Monoclonal Antibody (SK3), RPE-Astral™616 Conjugate (red).
Western detection of human CD81 in MCF-7 cell lysates using CD81 Mouse Monoclonal Antibody (5A6) followed by goat anti-mouse CF®680. M: 1.5 uL Peacock™ Prestained Protein Marker. Lane 1: 10 ug MCF-7 cell lysate. Lane 2: 1 ug MCF-7 cell lysate. The blots were imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel.

Full List of Biotium Choice Antibodies

Biotium Choice AntibodyCF®405S
(411/431 nm)
CF®405M
(416/452 nm)
CF®405L
(413/547 nm)
CF®488A
(490/516 nm)
CF®503R
(503/532 nm)
CF®514
(516/549 nm)
CF®568
(562/584 nm)
CF®575
(575/601 nm)
CF®583R
(585/609 nm)
CF®647Plus
(652/668 nm)
CF®660C
(667/685 nm)
CF®700
(696/721 nm)
R-PE
(496, 546, 565/576 nm)
RPE-Astral™616
(496, 546, 566/617 nm)
APC-Astral™813
(633, 638/813 nm)
APC
(651/660 nm)
Purified
(BSA free)
CD3e Monoclonal Mouse (SK7)P002-405SP002-405MP002-405LP002-488AP002-503RP002-514P002-568P002-575P002-647PP002-660CP002-700P002-RPEP002-R616P002-APC
CD4 Monoclonal Mouse (SK3)P001-405SP001-405MP001-405LP001-488AP001-503RP001-514P001-568P001-575P001-647PP001-660CP001-700P001-RPEP001-R616P001-A813P001-APC
CD8a Monoclonal Mouse (SK1)P009-405SP009-405MP009-405LP009-488AP009-503RP009-514P009-568P009-575P009-647PP009-660CP009-700P009-RPEP009-R616P009-A813P009-APC
CD9 Monoclonal Mouse (HI9a)P003S-RPEP003S-R616P003S-A813
CD9 Recombinant Monoclonal Mouse (2310.9)P015-405MP015-488AP015-568P015-583RP015-647PP015-700P015-RPEP015-R616P015-A813P015-APCP015
CD9 (Mouse) Recombinant Monoclonal Rat (24MS04.9)P016
CD16 Monoclonal Mouse (3G8)P011-405MP011-488AP011-568P011-647PP011-700P011-RPEP011-R616P011-A813
CD19 Monoclonal Mouse (SJ25C1)P010-405MP010-488AP010-575P010-647PP010-700P010-RPEP010-R616P010-A813
CD45 Monoclonal Mouse (2D1)P012-405MP012-488AP012-647PP012-RPEP012-R616P012-A813P012-APC
CD63 Monoclonal Mouse (H5C6)P004S-RPEP004-200uL
CD63 Recombinant Monoclonal Mouse (2304.63)P013-405MP013-488AP013-568P013-583RP013-647PP013-700P013-RPEP013-APCP013
CD81 Monoclonal Mouse (5A6)P005S-RPEP005S-R616
CD81 Recombinant Monoclonal Mouse (2304.81)P014-405MP014-488AP014-568P014-583RP014-647PP014-700P014-RPEP014-R616P014-APCP014
CD81 (Mouse/Rat) Recombinant Monoclonal Hamster (24MS04.81)P017

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EXPLORE OUR FULL PRIMARY ANTIBODY CATALOG

Discover Our Growing Collection of Over 2000 mAbs

  • Choose from antibodies validated for IHC, IF, flow, and western blot
  • Available in 6 bright and photostable CF® Dyes or biotin
  • Available BSA-free, ready to use with Mix-n-Stain™ Antibody Labeling Kits
  • Affordable 100 uL sizes available

Your Choice of Sizes and Formats

FormatConcentrationSize
CF® Dye conjugates (6 colors)0.1 mg/mL100 uL or 500 uL
Biotin conjugates0.1 mg/mL100 uL or 500 uL
Purified, with BSA0.2 mg/mL100 uL or 500 uL
Purified, BSA-free (Mix-n-Stain™ Ready)1 mg/mL50 uL

Browse our catalog or click below to use our Antibody Finder

Can’t find the right antibody conjugate? Request a custom conjugation below.

Formalin-fixed, paraffin-embedded prostate carcinoma stained with monoclonal anti-ODC-1 clone 485.
Formalin-fixed, paraffin-embedded prostate carcinoma stained with monoclonal anti-ODC-1 clone 485.
MCF7 cells stained with CF®555 anti-Cyclin B1 clone CCNB1/1098 (red), CF®488A phalloidin (green), and DAPI.
Flow cytometry analysis of Jurkat cells stained with CF®647 isotype (orange) or CF®647 anti-CD31 clone C31.7.
Western blot of HeLa cell lysate with CF®647 anti-nucleolin clone 364-5 imaged on a Typhoon® Trio scanner.

Primary Antibody Isotype Controls

Product PageHost speciesIsotypeClone
Mouse IgG1 KappaMouseIgG1, kappaIGG1/1331
Mouse IgG2a KappaMouseIgG2a, kappaMG2a-53
Mouse IgG2b KappaMouseIgG2b, kappaIGG/1333
Monoclonal Rabbit IgG Kappa RabbitIgG, kappaNCRBM/1520R

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BRIGHT FLUORESCENT CONJUGATES ACROSS THE SPECTRUM

CF® Dyes for Clearer Fluorescent Results

CF® Dyes are Biotium’s line of unique fluorescent probes that offer superior brightness and photostability compared to other commercially available fluorescent dyes. These performance advantages stem from unique chemical modifications aimed at optimizing biocompatibility and performance for the labeled conjugate. As a result, CF® Dyes offer exceptional signal-to-noise for a wide range of applications including western blotting, fluorescence microscopy, and flow cytometry. The dyes are available in a growing collection of 40 colors from blue to near-IR.

Exceptional Tandem Dye Performance with Astral Leap™

Astral Leap™ dyes are novel long Stokes shift tandem dyes that enable expanded multiplexing for flow cytometry, while offering exceptional signal-to-noise. Using breakthrough chemical strategies, our industry-leading chemists developed Astral Leap™ tandem dyes by optimizing both the Förster Resonance Energy Transfer (FRET) dye capabilities and conjugation to the fluorescent protein. RPE-Astral™616 and APC-Astral™813 are currently available with validated Biotium Choice primary antibodies, with more color options coming soon.

CF® Dyes are available in more than 40 bright and photostable fluorescent colors from blue to near-IR.

EverBrite™ Mounting Medium

From: $153 Sizes: 10 mLCatalog #:
23001
, - 23002View allHide

EverBrite™ Hardset Mounting Medium

From: $172 Sizes: 10 mLCatalog #:
23003
, - 23004View allHide

Drop-n-Stain EverBrite™ Mounting Medium

From: $39 Sizes: 2 mL, 10 mLCatalog #:
23008-T
, 23009-T, 23009, - 23008View allHide

CoverGrip™ Coverslip Sealant

From: $42 Sizes: 15 mL, 100 mLCatalog #:
23005
, - 23005-1View allHide

TrueBlack® Lipofuscin Autofluorescence Quencher

From: $161 Sizes: 1 mLCatalog #: 23007

TrueBlack® WB Blocking Buffer Kit

From: $89 Sizes: 10 membranes, 50 membranesCatalog #:
23013
, - 23013-TView allHide

TrueBlack® IF Background Suppressor System (Permeabilizing)

From: $52 Sizes: 20 assays, 200 assaysCatalog #:
23012
, - 23012-TView allHide

TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO

From: $61 Sizes: 50 uL, 500 uLCatalog #:
23014
, - 23014-TView allHide

NucSpot® Nuclear Stains

From: $90 Sizes: 20 uL, 100 uL, 200 uLCatalog #:
41040
, 41040-T, 41033, 41033-T, 41036, 41036-T, 41037, 41037-T, 41034, 41034-T, 41035, 41035-T, 41038, - 41038-TView allHide

Flow Cytometry Fixation/Permeabilization Kit

From: $34 Sizes: 50 testsCatalog #: 23006

Fixation Buffer

From: $117 Sizes: 100 mLCatalog #: 22015

Permeabilization Buffer

From: $117 Sizes: 100 mLCatalog #: 22016

Faq

Product shipping, storage, shelf life, & solubility

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.  


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Bioscience kits The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples. Antibodies and other conjugates The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). Chemicals, dyes, and gel stains Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage. If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples. Expiration date based on date of manufacture (DOM) If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance. Chemical products with special stability considerations: Esters Ester compounds include the following: • Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes • Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes • Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound. A note on CF® Dye succinimidyl ester stability Succinimidyl esters (SE) are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Many commercially available fluorescent dyes used for life science research are heavily sulfonated dyes which makes them particularly hygroscopic, worsening the hydrolysis problem. In addition, for several commercially available SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while the SE group in all of Biotium’s CF® Dyes is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF® Dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes. Maleimides, MTS and thiosulfate dyes Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions. Other reactive dyes Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information. Coelenterazines and D-luciferin Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution. Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution. Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced. D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.


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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions. Some products are shipped with blue ice packs as an extra precaution against high temperatures. The blue ice packs may be thawed upon arrival without affecting product performance. Products with recommended storage at ultra low temperature (-70°C) that also ship on dry ice should arrive frozen. If a product you received was shipped on dry ice and thawed during transit, please contact customer service at order@biotium.com. You can also download our Product Storage Statement here.


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CF® Dyes

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples. One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.  


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See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.

CF® Dye Alternatives for Akoya Tyramides

Akoya TyramideEx/Em (nm)Biotium TyramideEx/Em (nm)Biotium Cat. No.NotesCF® Dye Features
TSA Coumarin402/443CF®405S404/43192197Much brighter & more
photostable alternative
CF®405S Features
Opal™ Polaris 480450/500CF®430426/49896053Recommended
for abundant targets
CF®430 Features
Opal™520494/525CF®488A490/51592171CF®488A Features
TSA Fluorescein494/517Fluorescein492/51496018Direct replacementSee CF®488A for a brighter &
much more photostable alternative
TSA TMR
TSA Cyanine 3
550/570Cyanine 555555/56596020Direct replacement
for Cyanine 3
See CF®555 & CF®568 for brighter
& more photostable alternatives.
CF®555555/56596021Brighter & more
photostable alternatives
CF®555 Features
CF®568562/58392173CF®568 Features
Opal™570550/570CF®550R551/57796077CF®550R Features
CF®555555/565
96021CF®555 Features
TSA Plus Cyanine 3.5581/596CF®583R586/60996085Brighter & more
photostable alternatives
CF®583R Features
CF®594593/61492174CF®594 Features
Opal™620588/616CF®583R586/60996085CF®583R Features
TSA Cyanine 5648/667CF®640R642/66292175Brighter & more
photostable alternative
CF®640R Features
CF®647650/665 96022Brighter alternativeCF®647 Features
CF®660R663/68292195Less cross-talk with visible red dyes;
bright & extremely photostable
CF®660R Features
TSA Plus Cyanine 5.5
Opal™690
673/692
676/694
CF®680R680/70192196Bright & extremely
photostable
CF®680R Features
Opal™ Polaris 780750/770CF®754745/78696090Recommended
for abundant targets
Unique NIR dye for TSA
TSA BiotinN/ABiotin-XXN/A92176Direct replacement
TSA Plus DNPN/ADNPN/A96019Direct replacement
TSA Plus DIGN/AN/AN/AN/AN/A
Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. Opal is a trademark of Akoya Biosciences.  


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Dyes that carry multiple negative charges can introduce background. Usually, this is more of a concern with labeled antibodies that carry many dyes, as opposed to a small toxin like bungarotoxin. When staining tissues, the endogenous autofluorescence of the tissue itself is often the most significant source of background. Endogenous fluorescence background in tissue is usually highest in the blue wavelengths (DAPI channel) and lowest in the far-red (Cy®5 channel). Our CF®633 bungarotoxin (catalog no. 00009) is a far-red conjugate for the Cy®5 channel with a low negative charge that should have low background from either the dye or autofluorescence. We test fluorescent bungarotoxin on rat skeletal muscle sections. While the tissue shows autofluorescence, the bungarotoxin staining of motor endplates is usually much brighter than the background for all of the dye colors we’ve tested.  However, if you are staining human tissue (especially brain), lipofuscin autofluorescence may be bright in all channels. This usually shows up as bright, punctate dots around cell nuclei. While we would usually recommend our TrueBlack® lipofuscin quenchers for human brain tissue, they are not compatible with bungarotoxin staining. We have, however, found that EverBrite TrueBlack® Mounting Medium (cat. no. 23017) can be used to mount skeletal muscle sections stained with bungarotoxin. Cy Dye is a registered trademark of Cytiva.


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