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February 24, 2022

Protocols Library

By: Shalini Sharma

Antibody Staining Protocols

Considerations for Immunofluorescence

Immunofluorescence Staining for Microscopy

Cell Surface Staining for Flow Cytometry

Intracellular Staining for Flow Cytometry

Troubleshooting Tips for Immunofluorescence


Western Blotting

Fluorescent Western Blotting


DNA Labeling

DNA Probe Labeling by PCR


Cell Staining

Staining Cells with Hoechst or DAPI

Using Pluronic® F-127 to Load Cells with Hydrophobic Dyes or AM Esters


Antibody & Protein Labeling

Succinimidyl Ester Labeling of Amines

Maleimide Labeling of Thiols

Aminooxy Labeling of Glycoproteins

Biotium Product Protocols

Looking for a product manual? Go to the Protocols and Supporting Documents section on the right side of the product page (see the picture below) to download Product Information Sheets (PI).

Where to find Product Information Sheets:



You can find Product Information Sheets/Protocols (PI), SDS, safety reports, flyers and other documents on our product ordering pages under Protocols & Supporting Documents. Click the image to enlarge.