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Dyes For Labeling Carbonyls & Carbohydrates

Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) react with aldehydes or ketones to form a stable oxime bond under mild conditions without the use of a reducing agent. Aminooxy can be used to label abasic sites in damaged DNA or carbonyl groups resulting from protein oxidation. They also can be used to label carbohydrates or glycoproteins after oxidation of the carbohydrates to form carbonyl groups. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Aminooxy reagents are superior labeling reagents to hydrazides, because they form more stable linkages.

Hydrazides also react with aldehydes or ketones, but form unstable hydrazone linkages. Hydrazides are non-toxic and highly water soluble, making them useful as fluid phase markers and fixable microinjectable cytoplasmic tracers.

Fluorescent CF® Dye Aminooxy and CF® Dye Hydrazide are available with a wide selection of bright and photostable colors. For 2D DIGE platform, we offer CF®DI reactive dyes, which have matched molecular weight and charge. We also provide a wide selection of classic dyes and biotin in aminooxy and hydrazide forms.

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    CF® Dye Aminooxy

    From: $463 Sizes: 1 mgCatalog #:
    92050
    , 92051, 92052, 92053, 92054, 92055, 92056, 92057, 92058, - 92059View allHide

    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.

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    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.

     
    Product

    CF® Dye Hydrazide

    From: $179 Sizes: 1 mgCatalog #:
    96063
    , 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 96024, - 96025View allHide

    Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

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    Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

     
    Product

    CF®DI Aminooxy

    From: $214 Sizes: 1 mgCatalog #:
    92177
    , 92178, - 92179View allHide

    CF®DI Aminooxy derivatives are useful for fluorescently labeling aldehyde/ketone groups on biomolecules such as polysaccharids, glycoproteins or antibodies to generate size- and charge-matched conjugates.

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    CF®DI Aminooxy derivatives are useful for fluorescently labeling aldehyde/ketone groups on biomolecules such as polysaccharids, glycoproteins or antibodies to generate size- and charge-matched conjugates.

     
    Product

    CF®DI Hydrazide

    From: $179 Sizes: 1 mgCatalog #:
    92164
    , 92165, 92166, 92184, - 92185View allHide

    CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

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    CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

     
    Product

    Cyanine Dye Alkyne

    From: $179 Sizes: 1 mgCatalog #:
    92100
    , - 92101View allHide

    Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.

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    Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.

     
    Product

    Cyanine Dye Aminooxy

    From: $297 Sizes: 1 mgCatalog #:
    96008
    , - 96009View allHide

    Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

     

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    Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

     

     
    Product

    Aminooxy-5(6)-FAM

    From: $201 Sizes: 5 mgCatalog #: 90103

    Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

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    Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

     
    Product

    Aminooxy-5(6)-ROX

    From: $201 Sizes: 5 mgCatalog #: 90104

    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

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    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

     
    Product

    Aminooxy-5(6)-TAMRA

    From: $201 Sizes: 5 mgCatalog #: 90105

    Aminooxy-5(6)-TAMRA reacts with aldehydes or ketones to form a stable oxime linkage under mild conditions. It can also be used to label abasic sites in damaged DNA.

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    Aminooxy-5(6)-TAMRA reacts with aldehydes or ketones to form a stable oxime linkage under mild conditions. It can also be used to label abasic sites in damaged DNA.

     
    Product

    Aniline, 10X in Acetate Buffer

    From: $65 Sizes: 10 mLCatalog #: 91057

    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

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    10 mL
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    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

     
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    ANTS

    From: $68 Sizes: 500 mgCatalog #: 90010

    ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage (1).

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    500 mg
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    ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage (1).

     
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    APTS

    From: $135 Sizes: 10 mgCatalog #: 90028

    APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

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    10 mg
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    APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

     
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    ARP

    From: $213 Sizes: 10 mgCatalog #: 90073

    ARP

    ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

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    10 mg
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    ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.