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Dyes For Labeling Carbonyls & Carbohydrates

Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) react with aldehydes or ketones to form a stable oxime bond under mild conditions without the use of a reducing agent. Aminooxy can be used to label abasic sites in damaged DNA or carbonyl groups resulting from protein oxidation. They also can be used to label carbohydrates or glycoproteins after oxidation of the carbohydrates to form carbonyl groups. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Aminooxy reagents are superior labeling reagents to hydrazides, because they form more stable linkages.

Hydrazides also react with aldehydes or ketones, but form unstable hydrazone linkages. Hydrazides are non-toxic and highly water soluble, making them useful as fluid phase markers and fixable microinjectable cytoplasmic tracers.

Fluorescent CF® Dye Aminooxy and CF® Dye Hydrazide are available with a wide selection of bright and photostable colors. For 2D DIGE platform, we offer CF®DI reactive dyes, which have matched molecular weight and charge. We also provide a wide selection of classic dyes and biotin in aminooxy and hydrazide forms.

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    Aminooxy-5(6)-FAM

    From: $173 Sizes: 5 mgCatalog #: 90103

    Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable hydrazone compound under mild conditions. Similar to ARP (90073), aminooxy-5(6)-FAM can be used to label abasic sites in damaged DNA.

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    Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable hydrazone compound under mild conditions. Similar to ARP (90073), aminooxy-5(6)-FAM can be used to label abasic sites in damaged DNA.

     

    Aminooxy-5(6)-ROX

    From: $173 Sizes: 5 mgCatalog #: 90104

    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable hydrazone compound under mild conditions. Similar to ARP (#90073),aminooxy-5(6)-ROX can be used to label abasic sites in damaged DNA.

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    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable hydrazone compound under mild conditions. Similar to ARP (#90073),aminooxy-5(6)-ROX can be used to label abasic sites in damaged DNA.

     

    Aminooxy-5(6)-TAMRA

    From: $173 Sizes: 5 mgCatalog #: 90105

    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to form a stable hydrazone compound under mild condition. Similar to ARP (#90073),aminooxy-5(6)-TAMRA can be used to label abasic sites in damaged DNA.

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    Aminooxy-5(6)-ROX reacts with aldehydes or ketones to form a stable hydrazone compound under mild condition. Similar to ARP (#90073),aminooxy-5(6)-TAMRA can be used to label abasic sites in damaged DNA.

     

    Aniline, 10X in Acetate Buffer

    From: $55 Sizes: 10 mLCatalog #: 91057

    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

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    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

     

    ANTS

    From: $58 Sizes: 500 mgCatalog #: 90010

    ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage (1).

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    ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage (1).

     

    APTS

    From: $116 Sizes: 10 mgCatalog #: 90028

    APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

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    APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

     

    ARP

    From: $171 Sizes: 10 mgCatalog #: 90073

    ARP

    ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

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    ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

     

    CF® Dye Aminooxy

    From: $410 Sizes: 1 mgCatalog #: 92050, 92051, 92052, 92053, 92054, 92055, 92056, 92057, 92058, - 92059View allHide

    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

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    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

     

    CF® Dye Hydrazide

    From: $158 Sizes: 1 mgCatalog #: 96063, 96064, , 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 92192, 96024, - 96025View allHide

    Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

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    Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

     

    CFDI Hydrazide

    From: $158 Sizes: 1 mgCatalog #: 92164, 92165, 92166, 92184, - 92185View allHide

    CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform.

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    CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform.

     

    CF®DI Aminooxy

    From: $189 Sizes: 1 mgCatalog #: 92177, 92178, - 92179View allHide

    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. The CF®DI dyes are designed to match for charge and molecular weight. When coupled to the carbonyl group of the proteins, the molecular weight of the labeled proteins are closed to each other and the pl of the labeled proteins remains the same.

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    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. The CF®DI dyes are designed to match for charge and molecular weight. When coupled to the carbonyl group of the proteins, the molecular weight of the labeled proteins are closed to each other and the pl of the labeled proteins remains the same.

     

    Cyanine Dye Alkyne

    From: $158 Sizes: 1 mgCatalog #: 92100, - 92101View allHide

    Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

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    Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

     

    Cyanine Dye Aminooxy

    From: $263 Sizes: 1 mgCatalog #: 96008, - 96009View allHide

    Cyanine dye with an aminooxy reactive group could be used to label a target molecule with an aldehyde or ketone group. Aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

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    Cyanine dye with an aminooxy reactive group could be used to label a target molecule with an aldehyde or ketone group. Aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.