Dyes For Labeling Carbonyls & Carbohydrates
Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) react with aldehydes or ketones to form a stable oxime bond under mild conditions without the use of a reducing agent. Aminooxy can be used to label abasic sites in damaged DNA or carbonyl groups resulting from protein oxidation. They also can be used to label carbohydrates or glycoproteins after oxidation of the carbohydrates to form carbonyl groups. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Aminooxy reagents are superior labeling reagents to hydrazides, because they form more stable linkages.
Hydrazides also react with aldehydes or ketones, but form unstable hydrazone linkages. Hydrazides are non-toxic and highly water soluble, making them useful as fluid phase markers and fixable microinjectable cytoplasmic tracers.
Fluorescent CF® Dye Aminooxy and CF® Dye Hydrazide are available with a wide selection of bright and photostable colors. For 2D DIGE platform, we offer CF®DI reactive dyes, which have matched molecular weight and charge. We also provide a wide selection of classic dyes and biotin in aminooxy and hydrazide forms.