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CF® Dye Hydrazide

Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

Product Attributes

Cellular localization

Fluid phase tracer

Cell permeability

Membrane impermeant

Chemical reactivity (reacts with)

Aldehydes/ketones

Functional group

Hydrazide

Colors

Blue, Green, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Bright, extremely water-soluble and non-toxic CF® dye hydrazides are excellent fluorescent tracers for neurons or gap junction studies. The cell impermeant tracer dyes can be introduced into cells by microinjection. CF® dye hydrazides can also be used to fluorescently label aldehyde or ketone groups of carbohydrates or glycoproteins molecules after peroxidation with periodate.

  • Fluorescently labeled low molecular weight, polar, non-toxic molecules; useful as aldehyde-fixable cell tracers.
  • Membrane impermeant, can be easily loaded into cells by microinjection.
  • Can be used to fluorescently label aldehyde or ketone groups of polysaccharides, carbohydrates, or glycoproteins.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent imaging.

For carbohydrates labeling applications, we recommend using CF® dye aminooxy forms, which are more reactive toward these groups than hydrazide forms. For fluorescence two-dimensional differential gel electrophoresis (2D-DIGE), we recommend CFDI hydrazides.

Superior CF® Dyes

CF® dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF Dye Hydrazides

ProductEx/EmSizeSKUPurchase
CF®350 Hydrazide347/448 nm1 mg92151Purchase 92151
CF®405S Hydrazide404/431 nm1 mg92183Purchase 92183
CF®430 Hydrazide426/498 nm1 mg96063Purchase 96063
CF®440 Hydrazide440/515 nm1 mg96064Purchase 96064
CF®488A Hydrazide490/515 nm1 mg92152Purchase 92152
CF®555 Hydrazide555/565 nm1 mg92153Purchase 92153
CF®568 Hydrazide562/583 nm1 mg92154Purchase 92154
CF®594 Hydrazide593/614 nm1 mg92158Purchase 92158
CF®633 Hydrazide630/650 nm1 mg92156Purchase 92156
CF®640R Hydrazide642/662 nm1 mg92157Purchase 92157
CF®647 Hydrazide650/665 nm1 mg92136Purchase 92136
CF®660R Hydrazide663/682 nm1 mg96024Purchase 96024
CF®680R Hydrazide680/701 nm1 mg96025Purchase 96025

References

1. Cellular and Molecular Bioengineering (2019)12:121–130. DOI: 10.1007/s12195-018-0555-6
2. Journal of Controlled Release (2017) 263: 46-56. DOI: 10.1016/j.jconrel.2017.02.025
3. Int Forum Allergy Rhinol. (2017) 7:177– 184. DOI: 10.1002/alr.21865
4. Nature (2016) 538 (7623):96-98. DOI: 10.1038/nature19770

Download a list of CF® dye references.

 

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    CF®DI Hydrazide

    CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

    CF® Dye Aminooxy

    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.

    Cyanine Dye Aminooxy

    Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies. We offer Cyanine 555 and Cyanine 647, structurally identical to Cy®3 and Cy®5 respectively, from GE Healthcare.  

    Aniline, 10X in Acetate Buffer

    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

    CF® Dye Biocytin

    CF® dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.

    Ultrafiltration Vials

    Ultrafiltration devices for simple spin-column purification of proteins or other molecules by size separation with two molecular weight cut-off options: MWCO=10 kD (10K) and MWCO=3 kD (3K).