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CF® Dye Hydrazide

Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

Product Attributes

Cellular localization

Fluid phase tracer

Cell permeability

Membrane impermeant

Colors

Blue, Green, Red, Far-red, Near-infrared

Conjugation
Size
Catalog #
price
Qty
1 mg
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Product Description

Our CF® dye hydrazides are bright, extremely water-soluble and non-toxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies. The dyes can be introduced into cells by microinjection. Hydrazides also can be used for labeling aldehyde or ketone groups, such as carbohydrate molecules after peroxidation with periodate. However, for this application we recommend using CF® dye aminooxy forms, which are more reactive toward these groups than hydrazide forms. For 2D-DIGE platform, we recommend CFDI hydrazides.

CF Dye Hydrazide: Product & SKU Summary

ProductEx/EmSizeSKUPurchase
CF®350 Hydrazide347/448 nm1 mg92151Purchase 92151
CF®405S Hydrazide404/431 nm1 mg92183Purchase 92183
CF®430 Hydrazide426/498 nm1 mg96063Purchase 96063
CF®440 Hydrazide440/515 nm1 mg96064Purchase 96064
CF®488A Hydrazide490/515 nm1 mg92152Purchase 92152
CF®555 Hydrazide555/565 nm1 mg92153Purchase 92153
CF®568 Hydrazide562/583 nm1 mg92154Purchase 92154
CF®594 Hydrazide593/614 nm1 mg92158Purchase 92158
CF®633 Hydrazide630/650 nm1 mg92156Purchase 92156
CF®640R Hydrazide642/662 nm1 mg92157Purchase 92157
CF®647 Hydrazide650/665 nm1 mg92136Purchase 92136
CF®660R Hydrazide663/682 nm1 mg96024Purchase 96024
CF®680R Hydrazide680/701 nm1 mg96025Purchase 96025
CF®770 Hydrazide770/797 nm1 mg92192Purchase 92192

References

J Proteomics Computational Biol 2(1): 12 (2016)

Download a list of CF® dye references.

 

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    CFDI Hydrazide

    CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform.

    CF® Dye Aminooxy

    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

    Cyanine Dye Aminooxy

    Cyanine dye with an aminooxy reactive group could be used to label a target molecule with an aldehyde or ketone group. Aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

    Aniline, 10X in Acetate Buffer

    Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

    CF® Dye Biocytin

    CF® dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.

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