Menu
biotium logo
Antibody Finder
Menu
September 26, 2013

Can I fix NucView® Caspase-3 Substrates for subsequent immunostaining?

By: Rhonda Sorensen

Yes. We recommend a gentle fix in 2-4% paraformaldehyde for 10-15 minutes. Over-fixing can cause the signal to decrease. NucView® 488 staining can withstand permeabilization with 0.1% Triton X-100. Methanol fixation is not recommended.