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Dead cell stains

Dead cell stains are membrane-impermeant dyes that are excluded by viable cells, but stain necrotic or late apoptotic cells with damaged plasma membranes. Many of our dead cell stains are fluorogenic nucleic acid dyes that only fluoresce when bound to DNA or RNA inside dead cells, eliminating the need to wash before detection. See our full selection of nuclear stains on our Cellular Stains Technology Page.

Most DNA binding dead-cell stains are not fixable, meaning that the dyes will diffuse from stained cells to unstained cells after fixation, resulting in lower signal and higher background. Our Live-or-Dye™ Fixable Viability Stains are membrane-impermeant reactive dyes that covalently label dead cells. While these stains require washing, the staining can withstand fixation and permeabilization for subsequent IF staining. They also are useful for excluding dead cells from flow cytometry analysis of cell surface staining. See our complete selection of Apoptosis & Viability Stains.

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    Live-or-Dye NucFix™ Red Staining Kit

    From: $62 Sizes: 50 assays, 200 assaysCatalog #: 32010, - 32010-TView allHide

    A chemically-reactive, dead cell nuclear stain for mammalian cells, yeast, or gram-negative bacteria.

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    A chemically-reactive, dead cell nuclear stain for mammalian cells, yeast, or gram-negative bacteria.

     

    Live-or-Dye™ Fixable Viability Staining Kits

    From: $65 Sizes: 50 assays, 200 assaysCatalog #: 32002-T, 32003-T, 32009-T, 32004-T, 32012-T, 32005-T, 32006-T, 32007-T, 32013-T, 32008-T, 32002, 32011-T, 32003, 32009, 32004, 32012, 32005, 32006, 32007, 32013, 32008, - 32011View allHide

    Live-or-Dye™ Fixable Viability Staining Kits stain dead cells during flow cytometry to allow dead cells with permeable plasma membranes to be excluded from analysis.

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    Live-or-Dye™ Fixable Viability Staining Kits stain dead cells during flow cytometry to allow dead cells with permeable plasma membranes to be excluded from analysis.

     

    NucSpot® 470 Nuclear Stain, 1000X in DMSO

    Sizes: 20 uL, 200 uLCatalog #: 40083-T, - 40083View allHide

    NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.

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    NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.

     

    RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

    From: $75 Sizes: 25 uL (15-20 tests, trial size), 250 uL (150-200 tests), 1 mL (600-1000 tests)Catalog #: 40061, 40061-1, - 40061-TView allHide

    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

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    25 uL (15-20 tests, trial size)
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    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

     

    Ethidium Homodimer III (EthD-III)

    From: $249 Sizes: 1 mgCatalog #: 40050

    A red fluorescent dead cell stain for bacteria and mammalian cells

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    A red fluorescent dead cell stain for bacteria and mammalian cells

     

    Ethidium Homodimer III (EthD-III), 1 mM in DMSO

    From: $243 Sizes: 200 uLCatalog #: 40051

    A red fluorescent dead cell stain for bacteria and mammalian cells

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    A red fluorescent dead cell stain for bacteria and mammalian cells

     

    Oxazole Yellow, 1 mM in DMSO

    Sizes: 1 mLCatalog #: 40089

    Oxazole yellow, also know as YO-PRO-1, is a green-fluorescent, cell-impermeant nucleic acid stain with excitation/emission maxima of 491/509 nm when bound to nucleic acids. Oxazole yellow can be used as a early marker of apoptosis.

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    Oxazole yellow, also know as YO-PRO-1, is a green-fluorescent, cell-impermeant nucleic acid stain with excitation/emission maxima of 491/509 nm when bound to nucleic acids. Oxazole yellow can be used as a early marker of apoptosis.

     

    Thiazole Red Homodimer, 1mM in DMSO

    Sizes: 200 uLCatalog #: 40080

    Thiazole red homodimer (TRhD), also know as TOTO®-3, is a cell-impermeant, high-affinity, far-red fluorogenic nucleic acid stain.

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    Thiazole red homodimer (TRhD), also know as TOTO®-3, is a cell-impermeant, high-affinity, far-red fluorogenic nucleic acid stain.

     

    Thiazole Red, 1mM in DMSO

    Sizes: 1 mLCatalog #: 40087

    Thiazole red, also known as TO-PRO®-3, is a cell-impermeant, high-affinity far-red nucleic acid stain.

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    Thiazole red, also known as TO-PRO®-3, is a cell-impermeant, high-affinity far-red nucleic acid stain.

     

    TO iodide (515/531), 1 mM in DMSO

    Sizes: 1 mLCatalog #: 40088

    TO iodide, also know as TO-PRO®-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits green fluorescence when bound to nucleic acids.

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    TO iodide, also know as TO-PRO®-1, is a cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain that is essentially nonfluorescent in the absence of nucleic acids but exhibits green fluorescence when bound to nucleic acids.

     

    Oxazole Blue, 1 mM in DMSO

    Sizes: 1 mLCatalog #: 40091

    Oxazole blue, also known as PO-PRO™-1, is a blue-fluorescent dead cell stain. This dye is a cell-impermeant nucleic acid stain with excitation/emission maxima of 435/455 nm when bound to nucleic acids.

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    Oxazole blue, also known as PO-PRO™-1, is a blue-fluorescent dead cell stain. This dye is a cell-impermeant nucleic acid stain with excitation/emission maxima of 435/455 nm when bound to nucleic acids.

     

    7-AAD

    Sizes: 1 mL (1 mg/mL), 1 mgCatalog #: 40084, - 40037View allHide

    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.

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    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.

     

    NucSpot® Far-Red, 1000X in DMSO

    Sizes: 50 uL, 0.5 mLCatalog #: 40085, - 40085-TView allHide

    NucSpot® Far-Red was designed to be an improved alternative to 7-AAD for selective detection of dead cells by flow cytometry.

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    NucSpot® Far-Red was designed to be an improved alternative to 7-AAD for selective detection of dead cells by flow cytometry.

     

    Propidium Iodide (PI)

    Sizes: 10 mL (1 mg/mL in Water), 2 mL (50 ug/mL in Buffer), 100 mgCatalog #: 40016, 40017, - 40048View allHide

    Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

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    10 mL (1 mg/mL in Water)
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    Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.