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Apoptosis Assays

Biotium offers a wide range of fluorescent probes for studying apoptotic cells:

The NucView™ Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF™ Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.

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    NucView® 530 Caspase-3 Substrate, 1 mM in PBS

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10408, - 10408-TView allHide

    NucView® 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10407-T, - 10407View allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    MitoView™ Dyes

    From: $40 Sizes: Trial size (50 ug), 20 x 50 ugCatalog #: 70070, 70054, 70055, 70070-T, 70054-T, 70055-T, 70068, - 70068-TView allHide

    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

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    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

     

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10406, - 10406-TView allHide

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10405, - 10405-TView allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit

    From: $299 Sizes: 100 assaysCatalog #: 30072

    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

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    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

     

    Ac-DEVD-AMC

    From: $210 Sizes: 5 mgCatalog #: 10202

    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

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    5 mg
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    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

     

    5X Annexin V Binding Buffer

    From: $27 Sizes: 15 mLCatalog #: 99902

    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

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    15 mL
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    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

     

    Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30066

    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

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    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    Apoptosis and Necrosis Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30065

    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

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    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

     

    NucView® 488 and MitoView™ 633 Apoptosis Assay Kit

    From: $341 Sizes: 100 assaysCatalog #: 30062

    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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    100 assays
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    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

     

    CF®488A Annexin V and PI Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30061

    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

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    100 assays
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    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

     

    CF®488A Annexin V and 7-AAD Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30060

    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

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    100 assays
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    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

     

    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $92 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

    NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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    NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

     

    MCB Glutathione Detection Kit

    From: $323 Sizes: 100 assaysCatalog #: 30019

    MCB Glutathione Detection Kit utilizes a thiol-reactive dye monochlorobimane (MCB), which is essentially nonfluorescent until it reacts with a thiol to form a blue fluorescent product.

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    100 assays
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    MCB Glutathione Detection Kit utilizes a thiol-reactive dye monochlorobimane (MCB), which is essentially nonfluorescent until it reacts with a thiol to form a blue fluorescent product.

     

    Apoptotic, Necrotic, and Healthy Cells Quantification Kit

    From: $194 Sizes: 50 assaysCatalog #: 30018

    The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and all cells (blue) within the same cell population by flow cytometry or fluorescence microscopy.

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    50 assays
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    The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and all cells (blue) within the same cell population by flow cytometry or fluorescence microscopy.