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Apoptosis Assays

Biotium offers a wide range of fluorescent probes for studying apoptotic cells:

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.

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    5X Annexin V Binding Buffer

    From: $27 Sizes: 15 mLCatalog #: 99902

    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

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    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

     

    Ac-DEVD-AMC

    From: $210 Sizes: 5 mgCatalog #: 10202

    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

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    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

     

    Ac-DEVD-CHO Caspase-3 Inhibitor

    From: $68 Sizes: 1 mg, 5 mgCatalog #: 10404, - 10404-1View allHide

    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

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    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

     

    Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

    From: $60 Sizes: 5 ugCatalog #: 29012R-5ug, 29009R-5ug, 29083R-5ug, 29005R-5ug, 29004R-5ug, 29010R-5ug, 29011R-5ug, 29008R-5ug, 29014R-5ug, 29003R-5ug, - 29069R-5ugView allHide

    Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments.

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    5 ug
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    Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments.

     

    Annexin V Conjugates

    From: $89 Sizes: 100 uL, 500 uL, 0.5 mLCatalog #: 29001, 29002, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29057-500uL, - 29083View allHide

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

     

    Annexin V Near IR CF® Dye Conjugates

    From: $263 Sizes: 25 ugCatalog #: 29070, 29006, 29007, 29046, 29047, 29078, - 29082View allHide

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available labeled with our superior near-IR fluorescent dyes.

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    25 ug
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    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available labeled with our superior near-IR fluorescent dyes.

     

    Apoptosis and Necrosis Quantification Kit

    From: $179 Sizes: 50 assaysCatalog #: 30017

    The Apoptosis & Necrosis Quantification Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

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    The Apoptosis & Necrosis Quantification Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    Apoptosis and Necrosis Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30065

    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

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    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

     

    Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30066

    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

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    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    Apoptotic, Necrotic, and Healthy Cells Quantification Kit

    From: $194 Sizes: 50 assaysCatalog #: 30018

    The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and all cells (blue) within the same cell population by flow cytometry or fluorescence microscopy.

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    The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and all cells (blue) within the same cell population by flow cytometry or fluorescence microscopy.

     

    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $139 Sizes: 25 assays, 100 assaysCatalog #: 30008-1, - 30008-2View allHide

    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.

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    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.

     

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

    From: $46 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30009-1, 30009-2, - 30009-3View allHide

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.

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    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.

     

    Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit

    From: $139 Sizes: 25 assays, 100 assaysCatalog #: 30011-1, - 30011-2View allHide

    Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.

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    Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.

     

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

    From: $46 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30012-1, 30012-2, - 30012-3View allHide

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

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    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

     

    CF®488A Annexin V and 7-AAD Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30060

    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

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    100 assays
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    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

     

    CF®488A Annexin V and PI Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30061

    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

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    100 assays
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    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.