Yes, CellBrite® Cytoplasmic Membrane Dye staining can be done in buffer instead of culture medium. If you are staining adherent cells, we recommend using a buffer like HBSS with calcium/magnesium, which helps maintain cell adherence and morphology.
CellBrite® and MemBrite® Stains were originally developed for staining mammalian cells in culture, but some of the stains also have been validated for other organisms and applications. If you are staining a non-mammalian cell type or organism that grows in water or other medium, please see our Tech Tip: Researching Applications for Membrane Dyes. For dyes to stain yeast or bacteria membranes, see Cellular Stains in Different Organisms.
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