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Nucleic Acid Gel Stains

GelRed® & GelGreen® | EMBER500™ RNA Stain Gel-Bright™ Laser Diode Gel Illuminator

PCR & DNA Amplification

EvaGreen® Dye | Cheetah™ Taq Forget-Me-Not™ qPCR Master Mixes

RNA Research Solutions

EMBER500™ RNA Stain | AccuBlue® RNA Quant | RNAstorm™ RNA Extraction

Immunofluorescence Microscopy

EverBrite™ Mounting Medium Super-Resolution Microscopy Tyramide Signal Amplification

DNA & RNA Quantitation Kits

AccuGreen™ DNA for Qubit® | AccuClear® DNA AccuBlue® RNA

Cell Viability & Apoptosis

Cell Quantitation | Live & Dead Stains NucView® Caspase-3 Substrates

Cellular Stains

Nuclear Stains | MitoView™ Dyes Membrane/Cell Surface Stains

Extracellular Vesicle Research

ExoBrite™ EV Membrane Stains ExoBrite™ Antibody Conjugates

CF® Dyes

Reactive Dyes | Bioconjugates Near-IR CF® Dyes

Antibody & Protein Labeling Kits

Protein Labeling Kits | Small Ligand Labeling Mix-n-Stain™ Antibody Labeling Kits

Primary & Secondary Antibody Conjugates

Biotium Choice Antibodies | Secondary Antibodies Search with Antibody Finder

TrueBlack® Background Reducers

IF Background Suppressor | WB Blocking Buffer Autofluorescence Quencher

Flow Cytometry Reagents

ViaFluor® Cell Proliferation | Live-or-Dye™ Viability | Dyes for Spectral Flow

Luciferase Assays

Firefly Assay | Renilla Assay | Steady-Luc™

Protein Detection & Analysis

One-Step Protein Gel Stains | Western Blotting GloMelt™ Protein Thermal Stability Assay

RNA & DNA Extraction Kits

RNAstorm™ 2.0 FFPE | DNAstorm™ 2.0 FFPE RNAstorm™ Fresh Cell & Tissue

Viability PCR

PMA & PMAxx™ v-PCR Dyes | v-PCR Starter Kits Strain-Specific Kits

Microbiology

Bacterial Stains | Yeast Stains

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CF® Dye Selection Tool Cellular Stains Selection Guides Membrane & Cell Surface Stains Comparison Secondary Antibody Product Listings Antibody Finder Brochure Library

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Molecular Biology
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Free Precast GelRed® Agarose Gel Sample with Order
Bundle Deal for EV Stains & Antibodies
Exclusive Discount for Newsletter Subscribers
Free Sample for Newsletter Subscribers
New Lab Start-Up Program
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Overview of Antibody & Protein Labeling Kits
CF® Dye Protein Labeling Kits
Mix-n-Stain™ Antibody Labeling Kits
Mix-n-Stain™ Small Molecule Labeling Kits
VivoBrite™ Antibody Labeling Kits
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Overview of Apoptosis & Viability Assays
Proliferation & Viability Assays
Apoptosis Assays
Dead Cell Stains
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Overview of TrueBlack® Background Reducers
Blocking Buffers & Background Quenchers
Drugs, Inhibitors, & Chemicals
Filters & Filtration
Fixation, Permeabilization & Other Buffers
Laboratory Consumables
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Flourescence Spectra Viewer
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Request a Certificate of Analysis (CoA)

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Primary Antibodies for Cancer Research
NucView® Caspase-3 Substrates
Near-Infrared Dyes for In Vivo Imaging
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Luciferase Substrates
NucView® Caspase-3 Substrates
Peroxidase, Galactosidase, Phosphatase, & Other Substrates
Protease Substrates
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Overview of Flow Cytometry Reagents
Mix-n-Stain™ Antibody Labeling Kits
Apoptosis Assays
Dead Cell Stains
Proliferation & Viability Assays
Cell Cycle Analysis
Flow Cytometry Accessory Products
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Overview of CF® Dyes & Other Bioconjugates
Annexin V Conjugates
Alpha Bungarotoxin Conjugates
Cholera Toxin & Transferrin Conjugates
Lectin Conjugates
Dextrans & Other Fluid Phase Tracers
Biotin & Streptavidin Conjugates
Phalloidin Conjugates
Labeled Nucleotides
View all in Fluorescent Proteins, Nucleotides & Other Conjugates

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Near-IR CF® Dye Annexin V Conjugates
Near-IR Cytoplasmic Membrane Stains
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal Imaging
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Overview of Luciferase Assays
Firefly Luciferase Assay Kit 2.0
Renilla Luciferase Assay Kit 2.0
Firefly & Renilla Luciferase Single Tube Assay Kit
Steady-Luc™ Firefly HTS Assay Kit
Steady-Luc™ Firefly HTS Assay Kit (Lyophilized)
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Overview of PCR & DNA Amplification
qPCR Dyes
qPCR Master Mixes
Other qPCR Reagents
Viability PCR Reagents
EvaEZ™ Fluorometric Polymerase Activity Assay Kit
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Antibody Finder
Biotium Choice Antibodies
Primary Antibodies for Cancer Research
Primary Antibodies for Organelles
Primary Antibodies for Exosomes/EVs
Primary Antibodies for Microbiology
Primary Antibodies for Neuroscience
Anti-Human Immunoglobulin
Isotype Controls
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Overview of Reactive Dyes & Biotin
Amine-Reactive Dyes
Azide, Alkyne, BCN, Tetrazine & TCO
Biotinylation Reagents
Dyes For Labeling Carbonyls & Carbohydrates
Dyes with an Amine Group
Free Dyes
Thiol-Reactive Dyes
Tyramides and Tyramide Signal Amplification Kits
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Nitric Oxide Synthase Inhibitors
Nitric Oxide (NO) Detection
Nitric Oxide (NO) Donors
Reactive Oxygen Species Indicators
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All Secondary Antibody Product Listings
CF® Dye-Labeled Secondary Antibodies
CF® Dye Single Label Conjugates for STORM
Anti-Epitope Tag & Anti-Biotin Antibodies
Anti-Human Immunoglobulin
Biotinylated Secondary Antibodies
Alkaline Phosphatase Conjugates
HRP Conjugates
R-PE & APC Labeled Antibodies
Biotin & Streptavidin Conjugates
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Overview of Probes & Reagents for Neuroscience
Amyloid & Neurodegeneration Stains
Nerve Terminal Dyes
Neuronal Tracers
Toxins & Receptor Probes
Primary Antibodies for Neuroscience
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Overview of Near-IR WB Antibodies, Buffers, and Normalization Dyes
Overview of Protein Gel Stains
TrueBlack® WB Blocking Buffer Kit
Blocking Buffers & Background Reducers
Protein Gel Stains
Protein Markers
HRP & Alkaline Phosphatase Conjugates
Chromogenic Substrates
View all in Western Blot & Protein Gels

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CF® Dye Selection Tool
Cellular Stains Selection Guides
Membrane & Cell Surface Stains Comparison
Secondary Antibody Product Listing
Antibody Finder
Brochure Library

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PCR & DNA Amplification
Nucleic Acid Gel Stains & Accessories
Nucleic Acid Quantitation & Extraction
Nucleic Acid Binding Dyes

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Overview of DNA Gel Stains & Accessories
Nucleic Acid Gel Stains
DNA Ladders
Electrophoresis Accessories
Gel-Bright™ Laser Diode Gel Illuminator
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Overview of DNA & RNA Quantitation
Overview of DNA & RNA Extraction
DNA Quantitation Kits
RNA Quantitation Kits
DNA/RNA Extraction Kits
View all in Nucleic Acid Quantitation & Extraction

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DNA/RNA Binding Dyes
Reactive DNA/RNA Binding Dyes
Nuclear Counterstains
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Antibody & Protein Labeling Kits
Reactive CF® Dyes, Other Reactive Dyes
& Biotinylation Reagents
Primary Antibodies
Secondary Antibodies, Anti-Tag Antibodies,
& Streptavidin
Fluorescent Proteins, Nucleotides & Other Conjugates

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Cell & Organelle Stains
Apoptosis & Viability Assays
Calcium & Other Ion Indicators
Reagents for Nitric Oxide (NO)
& Reactive Oxygen Species (ROS)

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Overview of Cell & Organelle Stains
Nuclear Stains & DNA/RNA Dyes
Cell Surface & Membrane Dyes
Dyes for Mitochondria & Other Organelles
Cytosolic Tracers
Cytoskeletal Stains
Exosome/EV stains
Receptor/Ion Channel Toxins & Probes
Primary Antibodies for Organelles
View all in Cell & Organelle Stains

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Overview of Fluorescent Ion Indicators
Calcium Indicators & Chelators
Chloride, pH, Zinc & Other Indicators
View all in Calcium & Other Ion Indicators

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Western Blot & Protein Gels
Protein Quantitation & Stability Assays

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Overview of Protein Detection & Analysis
AccuOrange™ Protein Quantitation Kit
GloMelt™ Thermal Shift Protein Stability Kit
View all in Protein Quantitation and Stability Assays

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Enzyme Substrates
Luciferase Reporter Gene Assays

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Microbiology
Neuroscience
Cancer Research

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Overview of Microbiology Stains & Kits
Microbiology Primary Antibodies
Bacterial Viability
Fluorescent Gram Stains
Other Bacterial Stains
Yeast Stains
Yeast Viability
Viability PCR Reagents
PMA-Lite™ LED Photolysis Device
View all in Microbiology

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Fluorescence Microscopy
Flow Cytometry
In Vivo Imaging
Equipment
Buffers, Accessories, & Background Quenchers

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Overview of Immunofluorescence Microscopy Reagents
Nuclear Counterstains
Mounting Media & Microscopy Consumables
Blocking Buffers & Background Quenchers
Tyramides & Tyramide Signal Amplification Kits
Super-Resolution Imaging
View all in Fluorescence Microscopy

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Gel-Bright™ Laser Diode Gel Illuminator
PMA-Lite™ 2.0 LED Photolysis Device
View all in Equipment

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Nucleic Acid Gel Stains
PCR & DNA Amplification
RNA Research Solutions
Immunofluorescence Microscopy
DNA & RNA Quantitation Kits
Cell Viability & Apoptosis
Cellular Stains
Extracellular Vesicle Research
CF® Dyes
Antibody & Protein Labeling Kits
Primary & Secondary Antibody Conjugates
TrueBlack® Background Reducers
Flow Cytometry Reagents
Luciferase Assays
Protein Detection & Analysis
RNA & DNA Extraction Kits
Viability PCR
Microbiology
Products For Education
Earth-Friendly Products

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GelRed® & GelGreen®
EMBER500™ RNA Stain
Gel-Bright™ Laser Diode Gel Illuminator

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EvaGreen® Dye
Cheetah™ Taq
Forget-Me-Not™ qPCR Master Mixes

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EMBER500™ RNA Stain
AccuBlue® RNA Quant
RNAstorm™ RNA Extraction

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EverBrite™ Mounting Medium
Super-Resolution Microscopy
Tyramide Signal Amplification

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AccuGreen™ DNA for Qubit®
AccuClear® DNA
AccuBlue® RNA

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Cell Quantitation
Live & Dead Stains
NucView® Caspase-3 Substrates

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Nuclear Stains
MitoView™ Dyes
Membrane/Cell Surface Stains

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ExoBrite™ EV Membrane Stains
ExoBrite™ Antibody Conjugates

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Reactive Dyes
Bioconjugates
Near-IR CF® Dyes

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Protein Labeling Kits
Small Ligand Labeling
Mix-n-Stain™ Antibody Labeling Kits

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Biotium Choice Antibodies
Secondary Antibodies
Search with Antibody Finder

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IF Background Suppressor
WB Blocking Buffer
Autofluorescence Quencher

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ViaFluor® Cell Proliferation
Live-or-Dye™ Viability
Dyes for Spectral Flow

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Firefly Assay
Renilla Assay
Steady-Luc™

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One-Step Protein Gel Stains
Western Blotting
GloMelt™ Protein Thermal Stability Assay

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RNAstorm™ 2.0 FFPE
DNAstorm™ 2.0 FFPE
RNAstorm™ Fresh Cell & Tissue

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PMA & PMAxx™ v-PCR Dyes
v-PCR Starter Kits
Strain-Specific Kits

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Bacterial Stains
Yeast Stains

FAQs

FAQ CATEGORIES

Apoptosis, Necrosis, and Cell Viability Kits CellBrite® & MemBrite® Membrane & Cell Surface Stains CF® Dyes DNA & RNA Quantitation Kits DNAzure® Visible DNA Gel Stain EvaGreen® Dye and Master Mixes EverBrite™ Mounting Medium & CoverGrip™ Coverslip Sealant Exosome & EV Staining GelRed® and GelGreen® Nucleic Acid Gel Stains GelRed® and GelGreen® Troubleshooting Ion Indicators & Chelators Luciferase Assays Microbiology: Dyes for bacteria and yeast Mix-n-Stain™ Antibody Labeling Kits NucView® Caspase-3 Enzyme Substrates Organelle & Cytoskeleton Stains Other DNA/RNA Gel Stains PMA and PMAxx™ for viability PCR Product shipping, storage, shelf life, & solubility Protein Detection & Analysis Resazurin Cell Viability Assay RNAstorm™ and DNAstorm™ FFPE extraction kits Secondary Antibodies TrueBlack® Background Reducers Tyramide Signal Amplification (CF® Dye & Other Tyramides)

Ion Indicators & Chelators

Are the calcium sensors compatible with immunofluorecence?

BAPTA-based calcium indicators and chelators have been shown to be fixable in situ by EDC (EDAC)¹, #59002 which would be compatible with subsequent immunofluorescence and IHC.

¹Cell Calcium. 1997. 21(3), 175; DOI:10.1016/S0143-4160(97)90042-7.

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