The Mix-n-Stain™ kits do not specifically label the Fc region of the antibody, they will label accessible reactive residues irrespective of the location on the antibody. The image of the labeled dye on Fc region of antibody is merely representative. We design our Mix-n-Stain™ labeling kits to give the optimal DOL for each dye to minimize any effect on the binding affinity of the antibody. Based on this, it is likely that the majority of the labeling is on the Fc region of the antibody, which does not directly bind antigen. There are some cases where antibody labeling can disrupt antibody-antigen binding, usually for monoclonal antibodies, but this is rare based on our experience.
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