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April 19, 2022

How can I evaluate the integrity of the DNA I obtained?

By: Jacqueline Steenhuis

Due to the wide size distribution of DNA isolated from FFPE tissue samples, we recommend using pulsed-field gel electrophoresis (PFGE). Methods based on capillary electrophoresis such as the Agilent Bioanalyzer can also be used but may not properly resolve high molecular weight fragments (greater than 10kb) in better-quality samples.