The mechanism of action of TrueBlack® and TrueBlack® Plus Lipofuscin Autofluorescence Quenchers is similar to the traditionally used dye Sudan Black B. Lipofuscin consists of autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of cells as a consequence of aging. The TrueBlack® lipofuscin quenchers are hydrophobic in nature and associate with the lipid rich lipofuscin to quench autofluorescence.
TrueBlack® and TrueBlack® Plus Lipofuscin Quenchers have similar properties as Sudan Black B and, therefore, primarily stain lipofuscin. Like Sudan Black B, they may also stain some myeloid cells, as well as lipid droplets, but we have not confirmed this.
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