As with all fluorescence based probes, bleaching will occur over time. How long you can view NucView® staining under the microscope depends on several factors including the initial signal strength and the intensity of the excitation source. Some users have reported monitoring NucView® for 4-5 days. You can see a time-lapse video of NucView® staining over 24 hours here:
https://biotium.com/technology/nucview-caspase-3-substrates/
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