How should the tissue be prepared?

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Use a microtome to obtain 5-10 µm sections from FFPE samples. Sections thinner than 5 µm may be used if they can be reliably cut. Sections thicker than 10 µm are not recommended because they may not be fully digested. Also, no more than 5 sections (10 µm each) should be used for each extraction. Using too much tissue can lead to incomplete digestion and reduced yields.

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