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Fixation, Permeabilization & Other Buffers

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    Flow Cytometry Fixation/Permeabilization Kit

    From: $30 Sizes: 50 testsCatalog #: 23006

    A kit containing fixation and permeabilization buffers used in staining intracellular antigens for flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 tests
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    A kit containing fixation and permeabilization buffers used in staining intracellular antigens for flow cytometry.

     

    Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative

    From: $139 Sizes: 5 x 20 mLCatalog #: 22023

    A ready-to-use fixation solution for cells or tissues. Electron microscopy-grade, methanol-free paraformaldehyde, packaged under argon.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 x 20 mL
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    A ready-to-use fixation solution for cells or tissues. Electron microscopy-grade, methanol-free paraformaldehyde, packaged under argon.

     

    Fixation Buffer

    From: $96 Sizes: 100 mLCatalog #: 22015

    Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mL
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    Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

     

    Permeabilization and Blocking Buffer (5X)

    From: $96 Sizes: 100 mLCatalog #: 22017

    A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mL
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    A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

     

    Permeabilization Buffer

    From: $96 Sizes: 100 mLCatalog #: 22016

    Ready-to-use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 mL
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    Ready-to-use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

     

    Tween® 20

    From: $62 Sizes: 50 mLCatalog #: 22002

    A polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 mL
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    A polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays.

     

    AntiFix™ Universal Antigen Retrieval Buffer, 10X

    From: $80 Sizes: 50 mL, 500 mLCatalog #: 22030-50mL, - 22030-500mLView allHide

    AntiFix™ Universal Antigen Retrieval Buffer provides excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    500 mL
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    AntiFix™ Universal Antigen Retrieval Buffer provides excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization.

     

    Ready-to-Use Tyramide Amplification Buffer, 1X

    From: $102 Sizes: 25 mL, 100 mLCatalog #: 22027-25mL, - 22027-100mLView allHide

    For simple, optimized tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 mL
    100 mL
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    For simple, optimized tyramide amplification of immunostaining in cells or tissue sections. No need to add hydrogen peroxide before use.

     

    Tyramide Amplification Buffer Plus

    From: $110 Sizes: 250 reactions, 1000 reactionsCatalog #: 22029, - 22029-TView allHide

    An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    250 reactions
    1000 reactions
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    An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

     

    5X Annexin V Binding Buffer

    From: $30 Sizes: 15 mLCatalog #: 99902

    Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    15 mL
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    Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.

     

    5X Firefly Luciferase Lysis Buffer

    From: $21 Sizes: 15 mLCatalog #: 99923

    Additional concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kits when performing large sample volume assays.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    15 mL
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    Additional concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kits when performing large sample volume assays.

     

    5X Passive Lysis Buffer

    From: $27 Sizes: 30 mLCatalog #: 99912

    Additional concentrated lysis buffer for use with our Renilla Luciferase Assay Kits in large volume assays.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    30 mL
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    Additional concentrated lysis buffer for use with our Renilla Luciferase Assay Kits in large volume assays.

     

    Calcium Calibration Buffer Kit

    From: $164 Sizes: 1 kitCatalog #: 59100

    This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 kit
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    This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

     

    CHAPS, Ultra Pure

    From: $88 Sizes: 10 g, 100 gCatalog #: 22024-10g, - 22024-100gView allHide

    Zwitterionic non-denaturing detergent for solubilizing membrane proteins for protein gels and assays.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 g
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    Zwitterionic non-denaturing detergent for solubilizing membrane proteins for protein gels and assays.

     

    Pluronic® F-127

    From: $21 Sizes: 1 gCatalog #: 59000

    Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 g
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    Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

     

    Pluronic® F-127 20% Solution in DMSO

    From: $17 Sizes: 1 mLCatalog #: 59004

    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mL
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    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.