Labeled Nucleotides

An orange-red fluorescent 3′-end chain terminator in Sanger sequencing.

CF®640R-UTP can be enzymatically incorporated into RNA probes for use in many biological applications.

Labeled with our superior CF® dyes, dUTP conjugates can be used for TUNEL assay or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

The CF® dye TUNEL Assay Apoptosis Detection Kit label apoptotic cells in fixed tissue sections or cellular samples for fluorescence microscopy or flow cytometry.

Fluorescent dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5.

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.

5-Aminoallyl-UTP can be enzymatically incorporated into RNA for subsequent labeling with an amine-reactive fluorescent dye, biotin, or other hapten.

5-Aminoallyl-UTP can be enzymatically incorporated into RNA for subsequent labeling with an amine-reactive fluorescent dye, biotin, or other hapten via conventional peptide coupling methods.

5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells, and also is a good substrate for reverse transcriptase.

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-11-UTP can be enzymatically incorporated into RNA. The number 11 is the number of atoms in the linker between biotin and UTP.