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Reactive DNA/RNA Binding Dyes

Amine reactive succinimidyl ester (SE) of nucleic acid dyes can be conjugated to proteins or other molecules with primary amine groups. The conjugates should be essentially nonfluorescent until they bind DNA or RNA, resulting in fluorescence. We also offer reactive methylene blue, a colorimetric DNA/RNA stain.

PMA, PMAxx™, and EMA are nucleic acid binding dyes that covalently react with DNA and RNA after photoactivation. They are useful for PCR-based quantitation of microbial viability (learn more about viability PCR).

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    Product

    AO Succinimidyl Ester

    From: $315 Sizes: 5 mgCatalog #: 40074

    The amine reactive succinimidyl ester of AO (acridine orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates with green fluoroscence are able to complex with nucleic acids, resulting in green fluorescence nucleic acid conjugate adduct.

     
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    EB Succinimidyl Ester

    From: $315 Sizes: 5 mgCatalog #: 40072

    The amine reactive succinimidyl ester of EB (ethidium bromide) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in red fluorescence.

     
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    MB Succinimidyl Ester

    From: $315 Sizes: 5 mgCatalog #: 40075

    The amine reactive succinimidyl ester of MB (methylene blue) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

     
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    TO Succinimidyl Ester

    From: $315 Sizes: 5 mgCatalog #: 40073

    The amine reactive succinimidyl ester of TO (thiazole orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in green fluorescence.

     
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    PMAxx™ Dye, 20 mM in H2O

    From: $152 Sizes: 100 uLCatalog #: 40069

    Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).

     
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    PMA (Propidium Monoazide)

    From: $152 Sizes: 100 uL, 1 mgCatalog #:
    40013
    , - 40019View allHide

    A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.

     
    Product

    Ethidium Monoazide Bromide (EMA)

    From: $191 Sizes: 5 mgCatalog #: 40015

    A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.