Literature Digest: Validated Stains for Spheroids, Organoids, and Other 3D Cultures

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3D cell cultures—such as organoids and spheroids—are advanced, self-organizing tissue models that closely mimic the structure and function of real organs. Typically derived from stem cells or immortalized cell lines, these cutting-edge models offer researchers a powerful platform to replicate organ complexity or highlight specific biological processes in both ex vivo and in vivo settings.

As adoption of 3D culture systems accelerates across a wide range of research fields, so does the demand for high-performance, validated cellular stains that can deliver clear, reliable results.

In this digest, we spotlight six key publications that showcase how Biotium’s unique cellular stains are successfully applied in labeling cells within spheroids, organoids, and other 3D culture systems—providing you with actionable insights and proven approaches to enhance your own 3D culture studies.